Job ID = 14522148 SRX = SRX9906053 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 12183029 spots for SRR13494050/SRR13494050.sra Written 12183029 spots for SRR13494050/SRR13494050.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:09 12183029 reads; of these: 12183029 (100.00%) were unpaired; of these: 999375 (8.20%) aligned 0 times 10144301 (83.27%) aligned exactly 1 time 1039353 (8.53%) aligned >1 times 91.80% overall alignment rate Time searching: 00:03:09 Overall time: 00:03:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3905536 / 11183654 = 0.3492 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:21:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:21:36: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:21:36: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:21:44: 1000000 INFO @ Sat, 15 Jan 2022 22:21:52: 2000000 INFO @ Sat, 15 Jan 2022 22:22:00: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:22:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:22:06: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:22:06: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:22:08: 4000000 INFO @ Sat, 15 Jan 2022 22:22:15: 1000000 INFO @ Sat, 15 Jan 2022 22:22:17: 5000000 INFO @ Sat, 15 Jan 2022 22:22:24: 2000000 INFO @ Sat, 15 Jan 2022 22:22:26: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:22:33: 3000000 INFO @ Sat, 15 Jan 2022 22:22:35: 7000000 INFO @ Sat, 15 Jan 2022 22:22:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:22:36: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:22:36: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:22:37: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:22:37: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:22:37: #1 total tags in treatment: 7278118 INFO @ Sat, 15 Jan 2022 22:22:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:22:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:22:37: #1 tags after filtering in treatment: 7278118 INFO @ Sat, 15 Jan 2022 22:22:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:22:37: #1 finished! INFO @ Sat, 15 Jan 2022 22:22:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:22:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:22:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:22:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:22:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:22:42: 4000000 INFO @ Sat, 15 Jan 2022 22:22:46: 1000000 INFO @ Sat, 15 Jan 2022 22:22:50: 5000000 INFO @ Sat, 15 Jan 2022 22:22:56: 2000000 INFO @ Sat, 15 Jan 2022 22:22:59: 6000000 INFO @ Sat, 15 Jan 2022 22:23:07: 3000000 INFO @ Sat, 15 Jan 2022 22:23:08: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:23:10: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:23:10: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:23:10: #1 total tags in treatment: 7278118 INFO @ Sat, 15 Jan 2022 22:23:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:23:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:23:10: #1 tags after filtering in treatment: 7278118 INFO @ Sat, 15 Jan 2022 22:23:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:23:10: #1 finished! INFO @ Sat, 15 Jan 2022 22:23:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:23:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:23:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:23:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:23:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:23:17: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:23:27: 5000000 INFO @ Sat, 15 Jan 2022 22:23:37: 6000000 INFO @ Sat, 15 Jan 2022 22:23:47: 7000000 INFO @ Sat, 15 Jan 2022 22:23:49: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:23:49: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:23:49: #1 total tags in treatment: 7278118 INFO @ Sat, 15 Jan 2022 22:23:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:23:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:23:50: #1 tags after filtering in treatment: 7278118 INFO @ Sat, 15 Jan 2022 22:23:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:23:50: #1 finished! INFO @ Sat, 15 Jan 2022 22:23:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:23:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:23:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:23:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:23:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906053/SRX9906053.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling