Job ID = 14522146 SRX = SRX9906051 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 16437282 spots for SRR13494052/SRR13494052.sra Written 16437282 spots for SRR13494052/SRR13494052.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:29 16437282 reads; of these: 16437282 (100.00%) were unpaired; of these: 1798047 (10.94%) aligned 0 times 13136606 (79.92%) aligned exactly 1 time 1502629 (9.14%) aligned >1 times 89.06% overall alignment rate Time searching: 00:03:29 Overall time: 00:03:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5970101 / 14639235 = 0.4078 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:22:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:22:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:22:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:22:27: 1000000 INFO @ Sat, 15 Jan 2022 22:22:34: 2000000 INFO @ Sat, 15 Jan 2022 22:22:41: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:22:48: 4000000 INFO @ Sat, 15 Jan 2022 22:22:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:22:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:22:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:22:56: 5000000 INFO @ Sat, 15 Jan 2022 22:22:57: 1000000 INFO @ Sat, 15 Jan 2022 22:23:02: 6000000 INFO @ Sat, 15 Jan 2022 22:23:05: 2000000 INFO @ Sat, 15 Jan 2022 22:23:10: 7000000 INFO @ Sat, 15 Jan 2022 22:23:12: 3000000 INFO @ Sat, 15 Jan 2022 22:23:17: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:23:19: 4000000 INFO @ Sat, 15 Jan 2022 22:23:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:23:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:23:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:23:21: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:23:21: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:23:21: #1 total tags in treatment: 8669134 INFO @ Sat, 15 Jan 2022 22:23:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:23:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:23:21: #1 tags after filtering in treatment: 8669134 INFO @ Sat, 15 Jan 2022 22:23:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:23:21: #1 finished! INFO @ Sat, 15 Jan 2022 22:23:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:23:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:23:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:23:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:23:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:23:26: 5000000 INFO @ Sat, 15 Jan 2022 22:23:28: 1000000 INFO @ Sat, 15 Jan 2022 22:23:33: 6000000 INFO @ Sat, 15 Jan 2022 22:23:34: 2000000 INFO @ Sat, 15 Jan 2022 22:23:40: 7000000 INFO @ Sat, 15 Jan 2022 22:23:41: 3000000 INFO @ Sat, 15 Jan 2022 22:23:47: 8000000 INFO @ Sat, 15 Jan 2022 22:23:49: 4000000 INFO @ Sat, 15 Jan 2022 22:23:52: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:23:52: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:23:52: #1 total tags in treatment: 8669134 INFO @ Sat, 15 Jan 2022 22:23:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:23:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:23:52: #1 tags after filtering in treatment: 8669134 INFO @ Sat, 15 Jan 2022 22:23:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:23:52: #1 finished! INFO @ Sat, 15 Jan 2022 22:23:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:23:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:23:52: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:23:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:23:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:23:55: 5000000 INFO @ Sat, 15 Jan 2022 22:24:02: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:24:08: 7000000 INFO @ Sat, 15 Jan 2022 22:24:15: 8000000 INFO @ Sat, 15 Jan 2022 22:24:19: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:24:19: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:24:19: #1 total tags in treatment: 8669134 INFO @ Sat, 15 Jan 2022 22:24:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:24:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:24:19: #1 tags after filtering in treatment: 8669134 INFO @ Sat, 15 Jan 2022 22:24:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:24:19: #1 finished! INFO @ Sat, 15 Jan 2022 22:24:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:24:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:24:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:24:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:24:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906051/SRX9906051.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling