Job ID = 14522145 SRX = SRX9906050 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 14662167 spots for SRR13494053/SRR13494053.sra Written 14662167 spots for SRR13494053/SRR13494053.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:57 14662167 reads; of these: 14662167 (100.00%) were unpaired; of these: 991455 (6.76%) aligned 0 times 12259188 (83.61%) aligned exactly 1 time 1411524 (9.63%) aligned >1 times 93.24% overall alignment rate Time searching: 00:01:57 Overall time: 00:01:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5168718 / 13670712 = 0.3781 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:19:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:19:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:19:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:19:30: 1000000 INFO @ Sat, 15 Jan 2022 22:19:35: 2000000 INFO @ Sat, 15 Jan 2022 22:19:40: 3000000 INFO @ Sat, 15 Jan 2022 22:19:45: 4000000 INFO @ Sat, 15 Jan 2022 22:19:50: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:19:55: 6000000 INFO @ Sat, 15 Jan 2022 22:19:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:19:55: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:19:55: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:20:00: 7000000 INFO @ Sat, 15 Jan 2022 22:20:01: 1000000 INFO @ Sat, 15 Jan 2022 22:20:05: 8000000 INFO @ Sat, 15 Jan 2022 22:20:06: 2000000 INFO @ Sat, 15 Jan 2022 22:20:08: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:20:08: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:20:08: #1 total tags in treatment: 8501994 INFO @ Sat, 15 Jan 2022 22:20:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:20:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:20:08: #1 tags after filtering in treatment: 8501994 INFO @ Sat, 15 Jan 2022 22:20:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:20:08: #1 finished! INFO @ Sat, 15 Jan 2022 22:20:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:20:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:20:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:20:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:20:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:20:12: 3000000 INFO @ Sat, 15 Jan 2022 22:20:17: 4000000 INFO @ Sat, 15 Jan 2022 22:20:22: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:20:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:20:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:20:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:20:27: 6000000 INFO @ Sat, 15 Jan 2022 22:20:31: 1000000 INFO @ Sat, 15 Jan 2022 22:20:33: 7000000 INFO @ Sat, 15 Jan 2022 22:20:36: 2000000 INFO @ Sat, 15 Jan 2022 22:20:38: 8000000 INFO @ Sat, 15 Jan 2022 22:20:41: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:20:41: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:20:41: #1 total tags in treatment: 8501994 INFO @ Sat, 15 Jan 2022 22:20:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:20:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:20:41: #1 tags after filtering in treatment: 8501994 INFO @ Sat, 15 Jan 2022 22:20:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:20:41: #1 finished! INFO @ Sat, 15 Jan 2022 22:20:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:20:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:20:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:20:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:20:41: Process for pairing-model is terminated! INFO @ Sat, 15 Jan 2022 22:20:42: 3000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:20:47: 4000000 INFO @ Sat, 15 Jan 2022 22:20:52: 5000000 INFO @ Sat, 15 Jan 2022 22:20:57: 6000000 INFO @ Sat, 15 Jan 2022 22:21:02: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:21:07: 8000000 INFO @ Sat, 15 Jan 2022 22:21:10: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:21:10: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:21:10: #1 total tags in treatment: 8501994 INFO @ Sat, 15 Jan 2022 22:21:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:21:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:21:10: #1 tags after filtering in treatment: 8501994 INFO @ Sat, 15 Jan 2022 22:21:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:21:10: #1 finished! INFO @ Sat, 15 Jan 2022 22:21:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:21:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:21:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:21:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:21:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906050/SRX9906050.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。