Job ID = 14522123 SRX = SRX9906048 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 18173684 spots for SRR13494055/SRR13494055.sra Written 18173684 spots for SRR13494055/SRR13494055.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:37 18173684 reads; of these: 18173684 (100.00%) were unpaired; of these: 1761658 (9.69%) aligned 0 times 14328611 (78.84%) aligned exactly 1 time 2083415 (11.46%) aligned >1 times 90.31% overall alignment rate Time searching: 00:02:37 Overall time: 00:02:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6706004 / 16412026 = 0.4086 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:18:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:18:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:18:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:18:57: 1000000 INFO @ Sat, 15 Jan 2022 22:19:04: 2000000 INFO @ Sat, 15 Jan 2022 22:19:10: 3000000 INFO @ Sat, 15 Jan 2022 22:19:17: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:19:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:19:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:19:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:19:23: 5000000 INFO @ Sat, 15 Jan 2022 22:19:27: 1000000 INFO @ Sat, 15 Jan 2022 22:19:29: 6000000 INFO @ Sat, 15 Jan 2022 22:19:33: 2000000 INFO @ Sat, 15 Jan 2022 22:19:36: 7000000 INFO @ Sat, 15 Jan 2022 22:19:39: 3000000 INFO @ Sat, 15 Jan 2022 22:19:43: 8000000 INFO @ Sat, 15 Jan 2022 22:19:45: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:19:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:19:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:19:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:19:51: 9000000 INFO @ Sat, 15 Jan 2022 22:19:51: 5000000 INFO @ Sat, 15 Jan 2022 22:19:55: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:19:55: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:19:55: #1 total tags in treatment: 9706022 INFO @ Sat, 15 Jan 2022 22:19:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:19:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:19:55: #1 tags after filtering in treatment: 9706022 INFO @ Sat, 15 Jan 2022 22:19:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:19:55: #1 finished! INFO @ Sat, 15 Jan 2022 22:19:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:19:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:19:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:19:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:19:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:19:57: 1000000 INFO @ Sat, 15 Jan 2022 22:19:57: 6000000 INFO @ Sat, 15 Jan 2022 22:20:03: 2000000 INFO @ Sat, 15 Jan 2022 22:20:03: 7000000 INFO @ Sat, 15 Jan 2022 22:20:09: 3000000 INFO @ Sat, 15 Jan 2022 22:20:09: 8000000 INFO @ Sat, 15 Jan 2022 22:20:14: 4000000 INFO @ Sat, 15 Jan 2022 22:20:15: 9000000 INFO @ Sat, 15 Jan 2022 22:20:19: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:20:19: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:20:19: #1 total tags in treatment: 9706022 INFO @ Sat, 15 Jan 2022 22:20:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:20:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:20:19: #1 tags after filtering in treatment: 9706022 INFO @ Sat, 15 Jan 2022 22:20:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:20:19: #1 finished! INFO @ Sat, 15 Jan 2022 22:20:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:20:19: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:20:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:20:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:20:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:20:20: 5000000 INFO @ Sat, 15 Jan 2022 22:20:26: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:20:32: 7000000 INFO @ Sat, 15 Jan 2022 22:20:37: 8000000 INFO @ Sat, 15 Jan 2022 22:20:43: 9000000 INFO @ Sat, 15 Jan 2022 22:20:47: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:20:47: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:20:47: #1 total tags in treatment: 9706022 INFO @ Sat, 15 Jan 2022 22:20:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:20:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:20:47: #1 tags after filtering in treatment: 9706022 INFO @ Sat, 15 Jan 2022 22:20:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:20:47: #1 finished! INFO @ Sat, 15 Jan 2022 22:20:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:20:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:20:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:20:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:20:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906048/SRX9906048.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling