Job ID = 14522122 SRX = SRX9906047 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 17749386 spots for SRR13494056/SRR13494056.sra Written 17749386 spots for SRR13494056/SRR13494056.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:19 17749386 reads; of these: 17749386 (100.00%) were unpaired; of these: 1214453 (6.84%) aligned 0 times 14554226 (82.00%) aligned exactly 1 time 1980707 (11.16%) aligned >1 times 93.16% overall alignment rate Time searching: 00:02:19 Overall time: 00:02:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6397627 / 16534933 = 0.3869 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:18:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:18:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:18:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:18:33: 1000000 INFO @ Sat, 15 Jan 2022 22:18:38: 2000000 INFO @ Sat, 15 Jan 2022 22:18:43: 3000000 INFO @ Sat, 15 Jan 2022 22:18:48: 4000000 INFO @ Sat, 15 Jan 2022 22:18:53: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:18:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:18:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:18:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:18:58: 6000000 INFO @ Sat, 15 Jan 2022 22:19:04: 1000000 INFO @ Sat, 15 Jan 2022 22:19:05: 7000000 INFO @ Sat, 15 Jan 2022 22:19:10: 2000000 INFO @ Sat, 15 Jan 2022 22:19:11: 8000000 INFO @ Sat, 15 Jan 2022 22:19:16: 3000000 INFO @ Sat, 15 Jan 2022 22:19:17: 9000000 INFO @ Sat, 15 Jan 2022 22:19:23: 4000000 INFO @ Sat, 15 Jan 2022 22:19:23: 10000000 INFO @ Sat, 15 Jan 2022 22:19:24: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:19:24: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:19:24: #1 total tags in treatment: 10137306 INFO @ Sat, 15 Jan 2022 22:19:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:19:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:19:24: #1 tags after filtering in treatment: 10137306 INFO @ Sat, 15 Jan 2022 22:19:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:19:24: #1 finished! INFO @ Sat, 15 Jan 2022 22:19:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:19:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:19:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:19:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:19:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:19:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:19:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:19:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:19:28: 5000000 INFO @ Sat, 15 Jan 2022 22:19:34: 1000000 INFO @ Sat, 15 Jan 2022 22:19:35: 6000000 INFO @ Sat, 15 Jan 2022 22:19:41: 2000000 INFO @ Sat, 15 Jan 2022 22:19:42: 7000000 INFO @ Sat, 15 Jan 2022 22:19:48: 3000000 INFO @ Sat, 15 Jan 2022 22:19:48: 8000000 INFO @ Sat, 15 Jan 2022 22:19:54: 9000000 INFO @ Sat, 15 Jan 2022 22:19:54: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:20:01: 10000000 INFO @ Sat, 15 Jan 2022 22:20:01: 5000000 INFO @ Sat, 15 Jan 2022 22:20:01: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:20:01: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:20:01: #1 total tags in treatment: 10137306 INFO @ Sat, 15 Jan 2022 22:20:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:20:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:20:02: #1 tags after filtering in treatment: 10137306 INFO @ Sat, 15 Jan 2022 22:20:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:20:02: #1 finished! INFO @ Sat, 15 Jan 2022 22:20:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:20:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:20:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:20:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:20:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:20:08: 6000000 INFO @ Sat, 15 Jan 2022 22:20:14: 7000000 INFO @ Sat, 15 Jan 2022 22:20:20: 8000000 INFO @ Sat, 15 Jan 2022 22:20:26: 9000000 INFO @ Sat, 15 Jan 2022 22:20:33: 10000000 INFO @ Sat, 15 Jan 2022 22:20:33: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:20:33: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:20:33: #1 total tags in treatment: 10137306 INFO @ Sat, 15 Jan 2022 22:20:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:20:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:20:34: #1 tags after filtering in treatment: 10137306 INFO @ Sat, 15 Jan 2022 22:20:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:20:34: #1 finished! INFO @ Sat, 15 Jan 2022 22:20:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:20:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:20:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:20:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:20:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9906047/SRX9906047.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling