Job ID = 14521386 SRX = SRX9807984 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10011946 spots for SRR13388787/SRR13388787.sra Written 10011946 spots for SRR13388787/SRR13388787.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:29 10011946 reads; of these: 10011946 (100.00%) were paired; of these: 1665098 (16.63%) aligned concordantly 0 times 7426917 (74.18%) aligned concordantly exactly 1 time 919931 (9.19%) aligned concordantly >1 times ---- 1665098 pairs aligned concordantly 0 times; of these: 397120 (23.85%) aligned discordantly 1 time ---- 1267978 pairs aligned 0 times concordantly or discordantly; of these: 2535956 mates make up the pairs; of these: 1786336 (70.44%) aligned 0 times 522174 (20.59%) aligned exactly 1 time 227446 (8.97%) aligned >1 times 91.08% overall alignment rate Time searching: 00:05:29 Overall time: 00:05:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 337311 / 8738286 = 0.0386 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:06:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:06:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:06:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:06:14: 1000000 INFO @ Sat, 15 Jan 2022 21:06:18: 2000000 INFO @ Sat, 15 Jan 2022 21:06:23: 3000000 INFO @ Sat, 15 Jan 2022 21:06:27: 4000000 INFO @ Sat, 15 Jan 2022 21:06:32: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:06:37: 6000000 INFO @ Sat, 15 Jan 2022 21:06:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:06:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:06:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:06:42: 7000000 INFO @ Sat, 15 Jan 2022 21:06:43: 1000000 INFO @ Sat, 15 Jan 2022 21:06:48: 8000000 INFO @ Sat, 15 Jan 2022 21:06:48: 2000000 INFO @ Sat, 15 Jan 2022 21:06:52: 3000000 INFO @ Sat, 15 Jan 2022 21:06:52: 9000000 INFO @ Sat, 15 Jan 2022 21:06:56: 4000000 INFO @ Sat, 15 Jan 2022 21:06:57: 10000000 INFO @ Sat, 15 Jan 2022 21:07:01: 5000000 INFO @ Sat, 15 Jan 2022 21:07:02: 11000000 INFO @ Sat, 15 Jan 2022 21:07:05: 6000000 INFO @ Sat, 15 Jan 2022 21:07:07: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:07:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:07:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:07:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:07:10: 7000000 INFO @ Sat, 15 Jan 2022 21:07:11: 13000000 INFO @ Sat, 15 Jan 2022 21:07:13: 1000000 INFO @ Sat, 15 Jan 2022 21:07:14: 8000000 INFO @ Sat, 15 Jan 2022 21:07:16: 14000000 INFO @ Sat, 15 Jan 2022 21:07:18: 2000000 INFO @ Sat, 15 Jan 2022 21:07:19: 9000000 INFO @ Sat, 15 Jan 2022 21:07:21: 15000000 INFO @ Sat, 15 Jan 2022 21:07:22: 3000000 INFO @ Sat, 15 Jan 2022 21:07:23: 10000000 INFO @ Sat, 15 Jan 2022 21:07:26: 16000000 INFO @ Sat, 15 Jan 2022 21:07:27: 4000000 INFO @ Sat, 15 Jan 2022 21:07:28: 11000000 INFO @ Sat, 15 Jan 2022 21:07:31: 17000000 INFO @ Sat, 15 Jan 2022 21:07:31: 5000000 INFO @ Sat, 15 Jan 2022 21:07:32: 12000000 INFO @ Sat, 15 Jan 2022 21:07:33: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:07:33: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:07:33: #1 total tags in treatment: 8016417 INFO @ Sat, 15 Jan 2022 21:07:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:07:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:07:34: #1 tags after filtering in treatment: 6028323 INFO @ Sat, 15 Jan 2022 21:07:34: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 21:07:34: #1 finished! INFO @ Sat, 15 Jan 2022 21:07:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:07:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:07:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:07:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:07:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:07:36: 6000000 INFO @ Sat, 15 Jan 2022 21:07:37: 13000000 INFO @ Sat, 15 Jan 2022 21:07:40: 7000000 INFO @ Sat, 15 Jan 2022 21:07:41: 14000000 INFO @ Sat, 15 Jan 2022 21:07:45: 8000000 INFO @ Sat, 15 Jan 2022 21:07:46: 15000000 INFO @ Sat, 15 Jan 2022 21:07:49: 9000000 INFO @ Sat, 15 Jan 2022 21:07:50: 16000000 INFO @ Sat, 15 Jan 2022 21:07:54: 10000000 INFO @ Sat, 15 Jan 2022 21:07:55: 17000000 INFO @ Sat, 15 Jan 2022 21:07:57: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:07:57: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:07:57: #1 total tags in treatment: 8016417 INFO @ Sat, 15 Jan 2022 21:07:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:07:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:07:57: #1 tags after filtering in treatment: 6028323 INFO @ Sat, 15 Jan 2022 21:07:57: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 21:07:57: #1 finished! INFO @ Sat, 15 Jan 2022 21:07:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:07:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:07:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:07:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:07:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:07:58: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:08:03: 12000000 INFO @ Sat, 15 Jan 2022 21:08:07: 13000000 INFO @ Sat, 15 Jan 2022 21:08:11: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:08:16: 15000000 INFO @ Sat, 15 Jan 2022 21:08:20: 16000000 INFO @ Sat, 15 Jan 2022 21:08:24: 17000000 INFO @ Sat, 15 Jan 2022 21:08:27: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:08:27: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:08:27: #1 total tags in treatment: 8016417 INFO @ Sat, 15 Jan 2022 21:08:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:08:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:08:27: #1 tags after filtering in treatment: 6028323 INFO @ Sat, 15 Jan 2022 21:08:27: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 21:08:27: #1 finished! INFO @ Sat, 15 Jan 2022 21:08:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:08:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:08:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:08:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:08:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807984/SRX9807984.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling