Job ID = 14521385 SRX = SRX9807983 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10512567 spots for SRR13388786/SRR13388786.sra Written 10512567 spots for SRR13388786/SRR13388786.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:14 10512567 reads; of these: 10512567 (100.00%) were paired; of these: 1908049 (18.15%) aligned concordantly 0 times 7690813 (73.16%) aligned concordantly exactly 1 time 913705 (8.69%) aligned concordantly >1 times ---- 1908049 pairs aligned concordantly 0 times; of these: 495724 (25.98%) aligned discordantly 1 time ---- 1412325 pairs aligned 0 times concordantly or discordantly; of these: 2824650 mates make up the pairs; of these: 2015575 (71.36%) aligned 0 times 549031 (19.44%) aligned exactly 1 time 260044 (9.21%) aligned >1 times 90.41% overall alignment rate Time searching: 00:08:14 Overall time: 00:08:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 340734 / 9093499 = 0.0375 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:11:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:11:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:11:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:11:11: 1000000 INFO @ Sat, 15 Jan 2022 21:11:17: 2000000 INFO @ Sat, 15 Jan 2022 21:11:24: 3000000 INFO @ Sat, 15 Jan 2022 21:11:30: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:11:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:11:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:11:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:11:37: 5000000 INFO @ Sat, 15 Jan 2022 21:11:41: 1000000 INFO @ Sat, 15 Jan 2022 21:11:43: 6000000 INFO @ Sat, 15 Jan 2022 21:11:48: 2000000 INFO @ Sat, 15 Jan 2022 21:11:50: 7000000 INFO @ Sat, 15 Jan 2022 21:11:55: 3000000 INFO @ Sat, 15 Jan 2022 21:11:57: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:12:02: 4000000 INFO @ Sat, 15 Jan 2022 21:12:04: 9000000 INFO @ Sat, 15 Jan 2022 21:12:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:12:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:12:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:12:09: 5000000 INFO @ Sat, 15 Jan 2022 21:12:10: 10000000 INFO @ Sat, 15 Jan 2022 21:12:11: 1000000 INFO @ Sat, 15 Jan 2022 21:12:15: 6000000 INFO @ Sat, 15 Jan 2022 21:12:17: 11000000 INFO @ Sat, 15 Jan 2022 21:12:19: 2000000 INFO @ Sat, 15 Jan 2022 21:12:23: 7000000 INFO @ Sat, 15 Jan 2022 21:12:24: 12000000 INFO @ Sat, 15 Jan 2022 21:12:27: 3000000 INFO @ Sat, 15 Jan 2022 21:12:30: 8000000 INFO @ Sat, 15 Jan 2022 21:12:31: 13000000 INFO @ Sat, 15 Jan 2022 21:12:34: 4000000 INFO @ Sat, 15 Jan 2022 21:12:37: 9000000 INFO @ Sat, 15 Jan 2022 21:12:37: 14000000 INFO @ Sat, 15 Jan 2022 21:12:41: 5000000 INFO @ Sat, 15 Jan 2022 21:12:44: 10000000 INFO @ Sat, 15 Jan 2022 21:12:44: 15000000 INFO @ Sat, 15 Jan 2022 21:12:48: 6000000 INFO @ Sat, 15 Jan 2022 21:12:51: 11000000 INFO @ Sat, 15 Jan 2022 21:12:51: 16000000 INFO @ Sat, 15 Jan 2022 21:12:55: 7000000 INFO @ Sat, 15 Jan 2022 21:12:57: 12000000 INFO @ Sat, 15 Jan 2022 21:12:58: 17000000 INFO @ Sat, 15 Jan 2022 21:13:02: 8000000 INFO @ Sat, 15 Jan 2022 21:13:04: 13000000 INFO @ Sat, 15 Jan 2022 21:13:06: 18000000 INFO @ Sat, 15 Jan 2022 21:13:08: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:13:08: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:13:08: #1 total tags in treatment: 8272115 INFO @ Sat, 15 Jan 2022 21:13:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:13:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:13:08: #1 tags after filtering in treatment: 6238324 INFO @ Sat, 15 Jan 2022 21:13:08: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 21:13:08: #1 finished! INFO @ Sat, 15 Jan 2022 21:13:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:13:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:13:08: 9000000 INFO @ Sat, 15 Jan 2022 21:13:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:13:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:13:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:13:10: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:13:15: 10000000 INFO @ Sat, 15 Jan 2022 21:13:17: 15000000 INFO @ Sat, 15 Jan 2022 21:13:22: 11000000 INFO @ Sat, 15 Jan 2022 21:13:24: 16000000 INFO @ Sat, 15 Jan 2022 21:13:29: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:13:30: 17000000 INFO @ Sat, 15 Jan 2022 21:13:35: 13000000 INFO @ Sat, 15 Jan 2022 21:13:37: 18000000 INFO @ Sat, 15 Jan 2022 21:13:39: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:13:39: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:13:39: #1 total tags in treatment: 8272115 INFO @ Sat, 15 Jan 2022 21:13:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:13:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:13:40: #1 tags after filtering in treatment: 6238324 INFO @ Sat, 15 Jan 2022 21:13:40: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 21:13:40: #1 finished! INFO @ Sat, 15 Jan 2022 21:13:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:13:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:13:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:13:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:13:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:13:42: 14000000 INFO @ Sat, 15 Jan 2022 21:13:48: 15000000 INFO @ Sat, 15 Jan 2022 21:13:55: 16000000 INFO @ Sat, 15 Jan 2022 21:14:01: 17000000 INFO @ Sat, 15 Jan 2022 21:14:08: 18000000 INFO @ Sat, 15 Jan 2022 21:14:10: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:14:10: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:14:10: #1 total tags in treatment: 8272115 INFO @ Sat, 15 Jan 2022 21:14:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:14:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:14:10: #1 tags after filtering in treatment: 6238324 INFO @ Sat, 15 Jan 2022 21:14:10: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 21:14:10: #1 finished! INFO @ Sat, 15 Jan 2022 21:14:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:14:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:14:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:14:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:14:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807983/SRX9807983.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling