Job ID = 14521384 SRX = SRX9807982 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11011589 spots for SRR13388785/SRR13388785.sra Written 11011589 spots for SRR13388785/SRR13388785.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:14 11011589 reads; of these: 11011589 (100.00%) were paired; of these: 1941495 (17.63%) aligned concordantly 0 times 8126161 (73.80%) aligned concordantly exactly 1 time 943933 (8.57%) aligned concordantly >1 times ---- 1941495 pairs aligned concordantly 0 times; of these: 443594 (22.85%) aligned discordantly 1 time ---- 1497901 pairs aligned 0 times concordantly or discordantly; of these: 2995802 mates make up the pairs; of these: 2093692 (69.89%) aligned 0 times 644765 (21.52%) aligned exactly 1 time 257345 (8.59%) aligned >1 times 90.49% overall alignment rate Time searching: 00:08:14 Overall time: 00:08:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 408565 / 9507368 = 0.0430 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:10:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:10:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:10:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:11:00: 1000000 INFO @ Sat, 15 Jan 2022 21:11:05: 2000000 INFO @ Sat, 15 Jan 2022 21:11:10: 3000000 INFO @ Sat, 15 Jan 2022 21:11:14: 4000000 INFO @ Sat, 15 Jan 2022 21:11:19: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:11:23: 6000000 INFO @ Sat, 15 Jan 2022 21:11:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:11:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:11:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:11:28: 7000000 INFO @ Sat, 15 Jan 2022 21:11:31: 1000000 INFO @ Sat, 15 Jan 2022 21:11:33: 8000000 INFO @ Sat, 15 Jan 2022 21:11:36: 2000000 INFO @ Sat, 15 Jan 2022 21:11:39: 9000000 INFO @ Sat, 15 Jan 2022 21:11:41: 3000000 INFO @ Sat, 15 Jan 2022 21:11:44: 10000000 INFO @ Sat, 15 Jan 2022 21:11:47: 4000000 INFO @ Sat, 15 Jan 2022 21:11:49: 11000000 INFO @ Sat, 15 Jan 2022 21:11:52: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:11:54: 12000000 INFO @ Sat, 15 Jan 2022 21:11:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:11:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:11:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:11:57: 6000000 INFO @ Sat, 15 Jan 2022 21:12:00: 13000000 INFO @ Sat, 15 Jan 2022 21:12:02: 1000000 INFO @ Sat, 15 Jan 2022 21:12:03: 7000000 INFO @ Sat, 15 Jan 2022 21:12:06: 14000000 INFO @ Sat, 15 Jan 2022 21:12:08: 2000000 INFO @ Sat, 15 Jan 2022 21:12:09: 8000000 INFO @ Sat, 15 Jan 2022 21:12:12: 15000000 INFO @ Sat, 15 Jan 2022 21:12:14: 3000000 INFO @ Sat, 15 Jan 2022 21:12:15: 9000000 INFO @ Sat, 15 Jan 2022 21:12:18: 16000000 INFO @ Sat, 15 Jan 2022 21:12:20: 4000000 INFO @ Sat, 15 Jan 2022 21:12:21: 10000000 INFO @ Sat, 15 Jan 2022 21:12:24: 17000000 INFO @ Sat, 15 Jan 2022 21:12:26: 5000000 INFO @ Sat, 15 Jan 2022 21:12:28: 11000000 INFO @ Sat, 15 Jan 2022 21:12:31: 18000000 INFO @ Sat, 15 Jan 2022 21:12:33: 6000000 INFO @ Sat, 15 Jan 2022 21:12:34: 12000000 INFO @ Sat, 15 Jan 2022 21:12:37: 19000000 INFO @ Sat, 15 Jan 2022 21:12:38: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:12:38: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:12:38: #1 total tags in treatment: 8669953 INFO @ Sat, 15 Jan 2022 21:12:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:12:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:12:38: #1 tags after filtering in treatment: 6386464 INFO @ Sat, 15 Jan 2022 21:12:38: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 21:12:38: #1 finished! INFO @ Sat, 15 Jan 2022 21:12:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:12:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:12:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:12:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:12:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:12:39: 7000000 INFO @ Sat, 15 Jan 2022 21:12:40: 13000000 INFO @ Sat, 15 Jan 2022 21:12:45: 8000000 INFO @ Sat, 15 Jan 2022 21:12:46: 14000000 INFO @ Sat, 15 Jan 2022 21:12:51: 9000000 INFO @ Sat, 15 Jan 2022 21:12:52: 15000000 INFO @ Sat, 15 Jan 2022 21:12:57: 10000000 INFO @ Sat, 15 Jan 2022 21:12:58: 16000000 INFO @ Sat, 15 Jan 2022 21:13:03: 11000000 INFO @ Sat, 15 Jan 2022 21:13:04: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:13:08: 12000000 INFO @ Sat, 15 Jan 2022 21:13:09: 18000000 INFO @ Sat, 15 Jan 2022 21:13:14: 13000000 INFO @ Sat, 15 Jan 2022 21:13:15: 19000000 INFO @ Sat, 15 Jan 2022 21:13:16: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:13:16: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:13:16: #1 total tags in treatment: 8669953 INFO @ Sat, 15 Jan 2022 21:13:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:13:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:13:16: #1 tags after filtering in treatment: 6386464 INFO @ Sat, 15 Jan 2022 21:13:16: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 21:13:16: #1 finished! INFO @ Sat, 15 Jan 2022 21:13:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:13:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:13:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:13:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:13:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:13:20: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:13:25: 15000000 INFO @ Sat, 15 Jan 2022 21:13:31: 16000000 INFO @ Sat, 15 Jan 2022 21:13:36: 17000000 INFO @ Sat, 15 Jan 2022 21:13:42: 18000000 INFO @ Sat, 15 Jan 2022 21:13:47: 19000000 INFO @ Sat, 15 Jan 2022 21:13:48: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:13:48: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:13:48: #1 total tags in treatment: 8669953 INFO @ Sat, 15 Jan 2022 21:13:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:13:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:13:48: #1 tags after filtering in treatment: 6386464 INFO @ Sat, 15 Jan 2022 21:13:48: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 21:13:48: #1 finished! INFO @ Sat, 15 Jan 2022 21:13:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:13:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:13:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:13:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:13:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9807982/SRX9807982.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling