Job ID = 14519697 SRX = SRX9786335 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 19049067 spots for SRR13362167/SRR13362167.sra Written 19049067 spots for SRR13362167/SRR13362167.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:01 19049067 reads; of these: 19049067 (100.00%) were paired; of these: 8579958 (45.04%) aligned concordantly 0 times 9456034 (49.64%) aligned concordantly exactly 1 time 1013075 (5.32%) aligned concordantly >1 times ---- 8579958 pairs aligned concordantly 0 times; of these: 135154 (1.58%) aligned discordantly 1 time ---- 8444804 pairs aligned 0 times concordantly or discordantly; of these: 16889608 mates make up the pairs; of these: 9268506 (54.88%) aligned 0 times 6787526 (40.19%) aligned exactly 1 time 833576 (4.94%) aligned >1 times 75.67% overall alignment rate Time searching: 00:10:01 Overall time: 00:10:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 155785 / 10602826 = 0.0147 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:47:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:47:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:47:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:47:17: 1000000 INFO @ Sat, 15 Jan 2022 17:47:24: 2000000 INFO @ Sat, 15 Jan 2022 17:47:31: 3000000 INFO @ Sat, 15 Jan 2022 17:47:38: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:47:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:47:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:47:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:47:45: 5000000 INFO @ Sat, 15 Jan 2022 17:47:47: 1000000 INFO @ Sat, 15 Jan 2022 17:47:52: 6000000 INFO @ Sat, 15 Jan 2022 17:47:55: 2000000 INFO @ Sat, 15 Jan 2022 17:47:59: 7000000 INFO @ Sat, 15 Jan 2022 17:48:02: 3000000 INFO @ Sat, 15 Jan 2022 17:48:07: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:48:09: 4000000 INFO @ Sat, 15 Jan 2022 17:48:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:48:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:48:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:48:14: 9000000 INFO @ Sat, 15 Jan 2022 17:48:16: 1000000 INFO @ Sat, 15 Jan 2022 17:48:17: 5000000 INFO @ Sat, 15 Jan 2022 17:48:21: 10000000 INFO @ Sat, 15 Jan 2022 17:48:23: 2000000 INFO @ Sat, 15 Jan 2022 17:48:24: 6000000 INFO @ Sat, 15 Jan 2022 17:48:29: 11000000 INFO @ Sat, 15 Jan 2022 17:48:29: 3000000 INFO @ Sat, 15 Jan 2022 17:48:31: 7000000 INFO @ Sat, 15 Jan 2022 17:48:36: 4000000 INFO @ Sat, 15 Jan 2022 17:48:36: 12000000 INFO @ Sat, 15 Jan 2022 17:48:39: 8000000 INFO @ Sat, 15 Jan 2022 17:48:42: 5000000 INFO @ Sat, 15 Jan 2022 17:48:43: 13000000 INFO @ Sat, 15 Jan 2022 17:48:46: 9000000 INFO @ Sat, 15 Jan 2022 17:48:49: 6000000 INFO @ Sat, 15 Jan 2022 17:48:50: 14000000 INFO @ Sat, 15 Jan 2022 17:48:54: 10000000 INFO @ Sat, 15 Jan 2022 17:48:56: 7000000 INFO @ Sat, 15 Jan 2022 17:48:58: 15000000 INFO @ Sat, 15 Jan 2022 17:49:01: 11000000 INFO @ Sat, 15 Jan 2022 17:49:04: 8000000 INFO @ Sat, 15 Jan 2022 17:49:05: 16000000 INFO @ Sat, 15 Jan 2022 17:49:08: 12000000 INFO @ Sat, 15 Jan 2022 17:49:11: 9000000 INFO @ Sat, 15 Jan 2022 17:49:13: 17000000 INFO @ Sat, 15 Jan 2022 17:49:15: 13000000 INFO @ Sat, 15 Jan 2022 17:49:19: 10000000 INFO @ Sat, 15 Jan 2022 17:49:20: 18000000 INFO @ Sat, 15 Jan 2022 17:49:23: 14000000 INFO @ Sat, 15 Jan 2022 17:49:26: 11000000 INFO @ Sat, 15 Jan 2022 17:49:28: 19000000 INFO @ Sat, 15 Jan 2022 17:49:30: 15000000 INFO @ Sat, 15 Jan 2022 17:49:33: 12000000 INFO @ Sat, 15 Jan 2022 17:49:35: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:49:37: 16000000 INFO @ Sat, 15 Jan 2022 17:49:40: 13000000 INFO @ Sat, 15 Jan 2022 17:49:43: 21000000 INFO @ Sat, 15 Jan 2022 17:49:45: 17000000 INFO @ Sat, 15 Jan 2022 17:49:48: 14000000 INFO @ Sat, 15 Jan 2022 17:49:50: 22000000 INFO @ Sat, 15 Jan 2022 17:49:52: 18000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:49:55: 15000000 INFO @ Sat, 15 Jan 2022 17:49:57: 23000000 INFO @ Sat, 15 Jan 2022 17:49:59: 19000000 INFO @ Sat, 15 Jan 2022 17:50:03: 16000000 INFO @ Sat, 15 Jan 2022 17:50:05: 24000000 INFO @ Sat, 15 Jan 2022 17:50:07: 20000000 INFO @ Sat, 15 Jan 2022 17:50:10: 17000000 INFO @ Sat, 15 Jan 2022 17:50:13: 25000000 INFO @ Sat, 15 Jan 2022 17:50:14: 21000000 INFO @ Sat, 15 Jan 2022 17:50:17: 18000000 INFO @ Sat, 15 Jan 2022 17:50:20: 26000000 INFO @ Sat, 15 Jan 2022 17:50:21: 22000000 INFO @ Sat, 15 Jan 2022 17:50:24: 19000000 INFO @ Sat, 15 Jan 2022 17:50:28: 27000000 INFO @ Sat, 15 Jan 2022 17:50:28: 23000000 INFO @ Sat, 15 Jan 2022 17:50:31: 20000000 INFO @ Sat, 15 Jan 2022 17:50:35: 28000000 INFO @ Sat, 15 Jan 2022 17:50:36: 24000000 INFO @ Sat, 15 Jan 2022 17:50:38: 21000000 INFO @ Sat, 15 Jan 2022 17:50:39: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:50:39: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:50:39: #1 total tags in treatment: 10313825 INFO @ Sat, 15 Jan 2022 17:50:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:50:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:50:39: #1 tags after filtering in treatment: 7830663 INFO @ Sat, 15 Jan 2022 17:50:39: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 17:50:39: #1 finished! INFO @ Sat, 15 Jan 2022 17:50:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:50:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:50:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:50:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:50:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:50:43: 25000000 INFO @ Sat, 15 Jan 2022 17:50:44: 22000000 INFO @ Sat, 15 Jan 2022 17:50:50: 26000000 INFO @ Sat, 15 Jan 2022 17:50:51: 23000000 INFO @ Sat, 15 Jan 2022 17:50:58: 27000000 INFO @ Sat, 15 Jan 2022 17:50:58: 24000000 INFO @ Sat, 15 Jan 2022 17:51:05: 25000000 INFO @ Sat, 15 Jan 2022 17:51:05: 28000000 INFO @ Sat, 15 Jan 2022 17:51:09: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:51:09: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:51:09: #1 total tags in treatment: 10313825 INFO @ Sat, 15 Jan 2022 17:51:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:51:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:51:09: #1 tags after filtering in treatment: 7830663 INFO @ Sat, 15 Jan 2022 17:51:09: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 17:51:09: #1 finished! INFO @ Sat, 15 Jan 2022 17:51:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:51:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:51:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:51:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:51:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:51:11: 26000000 INFO @ Sat, 15 Jan 2022 17:51:17: 27000000 INFO @ Sat, 15 Jan 2022 17:51:23: 28000000 INFO @ Sat, 15 Jan 2022 17:51:26: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:51:26: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:51:26: #1 total tags in treatment: 10313825 INFO @ Sat, 15 Jan 2022 17:51:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:51:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:51:26: #1 tags after filtering in treatment: 7830663 INFO @ Sat, 15 Jan 2022 17:51:26: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 17:51:26: #1 finished! INFO @ Sat, 15 Jan 2022 17:51:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:51:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:51:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:51:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:51:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786335/SRX9786335.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling