Job ID = 14519696 SRX = SRX9786334 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 20696801 spots for SRR13362166/SRR13362166.sra Written 20696801 spots for SRR13362166/SRR13362166.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:53 20696801 reads; of these: 20696801 (100.00%) were paired; of these: 8847660 (42.75%) aligned concordantly 0 times 10830496 (52.33%) aligned concordantly exactly 1 time 1018645 (4.92%) aligned concordantly >1 times ---- 8847660 pairs aligned concordantly 0 times; of these: 251228 (2.84%) aligned discordantly 1 time ---- 8596432 pairs aligned 0 times concordantly or discordantly; of these: 17192864 mates make up the pairs; of these: 9562178 (55.62%) aligned 0 times 6848658 (39.83%) aligned exactly 1 time 782028 (4.55%) aligned >1 times 76.90% overall alignment rate Time searching: 00:10:53 Overall time: 00:10:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 178221 / 12098938 = 0.0147 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:49:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:49:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:49:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:49:10: 1000000 INFO @ Sat, 15 Jan 2022 17:49:14: 2000000 INFO @ Sat, 15 Jan 2022 17:49:19: 3000000 INFO @ Sat, 15 Jan 2022 17:49:23: 4000000 INFO @ Sat, 15 Jan 2022 17:49:27: 5000000 INFO @ Sat, 15 Jan 2022 17:49:32: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:49:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:49:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:49:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:49:36: 7000000 INFO @ Sat, 15 Jan 2022 17:49:40: 1000000 INFO @ Sat, 15 Jan 2022 17:49:41: 8000000 INFO @ Sat, 15 Jan 2022 17:49:45: 9000000 INFO @ Sat, 15 Jan 2022 17:49:46: 2000000 INFO @ Sat, 15 Jan 2022 17:49:50: 10000000 INFO @ Sat, 15 Jan 2022 17:49:51: 3000000 INFO @ Sat, 15 Jan 2022 17:49:54: 11000000 INFO @ Sat, 15 Jan 2022 17:49:56: 4000000 INFO @ Sat, 15 Jan 2022 17:49:59: 12000000 INFO @ Sat, 15 Jan 2022 17:50:01: 5000000 INFO @ Sat, 15 Jan 2022 17:50:03: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:50:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:50:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:50:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:50:06: 6000000 INFO @ Sat, 15 Jan 2022 17:50:08: 14000000 INFO @ Sat, 15 Jan 2022 17:50:10: 1000000 INFO @ Sat, 15 Jan 2022 17:50:11: 7000000 INFO @ Sat, 15 Jan 2022 17:50:13: 15000000 INFO @ Sat, 15 Jan 2022 17:50:16: 2000000 INFO @ Sat, 15 Jan 2022 17:50:16: 8000000 INFO @ Sat, 15 Jan 2022 17:50:17: 16000000 INFO @ Sat, 15 Jan 2022 17:50:21: 3000000 INFO @ Sat, 15 Jan 2022 17:50:22: 9000000 INFO @ Sat, 15 Jan 2022 17:50:22: 17000000 INFO @ Sat, 15 Jan 2022 17:50:26: 4000000 INFO @ Sat, 15 Jan 2022 17:50:26: 18000000 INFO @ Sat, 15 Jan 2022 17:50:27: 10000000 INFO @ Sat, 15 Jan 2022 17:50:31: 19000000 INFO @ Sat, 15 Jan 2022 17:50:31: 5000000 INFO @ Sat, 15 Jan 2022 17:50:32: 11000000 INFO @ Sat, 15 Jan 2022 17:50:36: 20000000 INFO @ Sat, 15 Jan 2022 17:50:36: 6000000 INFO @ Sat, 15 Jan 2022 17:50:37: 12000000 INFO @ Sat, 15 Jan 2022 17:50:40: 21000000 INFO @ Sat, 15 Jan 2022 17:50:41: 7000000 INFO @ Sat, 15 Jan 2022 17:50:42: 13000000 INFO @ Sat, 15 Jan 2022 17:50:45: 22000000 INFO @ Sat, 15 Jan 2022 17:50:46: 8000000 INFO @ Sat, 15 Jan 2022 17:50:48: 14000000 INFO @ Sat, 15 Jan 2022 17:50:49: 23000000 INFO @ Sat, 15 Jan 2022 17:50:52: 9000000 INFO @ Sat, 15 Jan 2022 17:50:53: 15000000 INFO @ Sat, 15 Jan 2022 17:50:54: 24000000 INFO @ Sat, 15 Jan 2022 17:50:57: 10000000 INFO @ Sat, 15 Jan 2022 17:50:58: 16000000 INFO @ Sat, 15 Jan 2022 17:50:58: 25000000 INFO @ Sat, 15 Jan 2022 17:51:02: 11000000 INFO @ Sat, 15 Jan 2022 17:51:03: 26000000 INFO @ Sat, 15 Jan 2022 17:51:03: 17000000 INFO @ Sat, 15 Jan 2022 17:51:07: 12000000 INFO @ Sat, 15 Jan 2022 17:51:08: 27000000 INFO @ Sat, 15 Jan 2022 17:51:08: 18000000 INFO @ Sat, 15 Jan 2022 17:51:12: 13000000 INFO @ Sat, 15 Jan 2022 17:51:12: 28000000 INFO @ Sat, 15 Jan 2022 17:51:14: 19000000 INFO @ Sat, 15 Jan 2022 17:51:17: 29000000 INFO @ Sat, 15 Jan 2022 17:51:17: 14000000 INFO @ Sat, 15 Jan 2022 17:51:19: 20000000 INFO @ Sat, 15 Jan 2022 17:51:21: 30000000 INFO @ Sat, 15 Jan 2022 17:51:22: 15000000 INFO @ Sat, 15 Jan 2022 17:51:24: 21000000 INFO @ Sat, 15 Jan 2022 17:51:26: 31000000 INFO @ Sat, 15 Jan 2022 17:51:28: 16000000 INFO @ Sat, 15 Jan 2022 17:51:28: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:51:28: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:51:28: #1 total tags in treatment: 11671776 INFO @ Sat, 15 Jan 2022 17:51:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:51:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:51:28: #1 tags after filtering in treatment: 8621316 INFO @ Sat, 15 Jan 2022 17:51:28: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 17:51:28: #1 finished! INFO @ Sat, 15 Jan 2022 17:51:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:51:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:51:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:51:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:51:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 17:51:29: 22000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:51:33: 17000000 INFO @ Sat, 15 Jan 2022 17:51:34: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:51:39: 18000000 INFO @ Sat, 15 Jan 2022 17:51:39: 24000000 INFO @ Sat, 15 Jan 2022 17:51:44: 19000000 INFO @ Sat, 15 Jan 2022 17:51:44: 25000000 INFO @ Sat, 15 Jan 2022 17:51:49: 20000000 INFO @ Sat, 15 Jan 2022 17:51:50: 26000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:51:54: 21000000 INFO @ Sat, 15 Jan 2022 17:51:55: 27000000 INFO @ Sat, 15 Jan 2022 17:51:59: 22000000 INFO @ Sat, 15 Jan 2022 17:52:00: 28000000 INFO @ Sat, 15 Jan 2022 17:52:04: 23000000 INFO @ Sat, 15 Jan 2022 17:52:05: 29000000 INFO @ Sat, 15 Jan 2022 17:52:09: 24000000 INFO @ Sat, 15 Jan 2022 17:52:10: 30000000 INFO @ Sat, 15 Jan 2022 17:52:14: 25000000 INFO @ Sat, 15 Jan 2022 17:52:15: 31000000 INFO @ Sat, 15 Jan 2022 17:52:18: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:52:18: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:52:18: #1 total tags in treatment: 11671776 INFO @ Sat, 15 Jan 2022 17:52:18: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:52:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:52:18: #1 tags after filtering in treatment: 8621316 INFO @ Sat, 15 Jan 2022 17:52:18: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 17:52:18: #1 finished! INFO @ Sat, 15 Jan 2022 17:52:18: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:52:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:52:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:52:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:52:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:52:20: 26000000 INFO @ Sat, 15 Jan 2022 17:52:25: 27000000 INFO @ Sat, 15 Jan 2022 17:52:30: 28000000 INFO @ Sat, 15 Jan 2022 17:52:35: 29000000 INFO @ Sat, 15 Jan 2022 17:52:40: 30000000 INFO @ Sat, 15 Jan 2022 17:52:45: 31000000 INFO @ Sat, 15 Jan 2022 17:52:48: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:52:48: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:52:48: #1 total tags in treatment: 11671776 INFO @ Sat, 15 Jan 2022 17:52:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:52:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:52:48: #1 tags after filtering in treatment: 8621316 INFO @ Sat, 15 Jan 2022 17:52:48: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 17:52:48: #1 finished! INFO @ Sat, 15 Jan 2022 17:52:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:52:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:52:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:52:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:52:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786334/SRX9786334.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling