Job ID = 14519695 SRX = SRX9786333 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 12593208 spots for SRR13362165/SRR13362165.sra Written 12593208 spots for SRR13362165/SRR13362165.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:03 12593208 reads; of these: 12593208 (100.00%) were paired; of these: 5497885 (43.66%) aligned concordantly 0 times 6520526 (51.78%) aligned concordantly exactly 1 time 574797 (4.56%) aligned concordantly >1 times ---- 5497885 pairs aligned concordantly 0 times; of these: 186301 (3.39%) aligned discordantly 1 time ---- 5311584 pairs aligned 0 times concordantly or discordantly; of these: 10623168 mates make up the pairs; of these: 5993386 (56.42%) aligned 0 times 4172241 (39.27%) aligned exactly 1 time 457541 (4.31%) aligned >1 times 76.20% overall alignment rate Time searching: 00:10:03 Overall time: 00:10:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 105672 / 7280786 = 0.0145 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:45:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:45:55: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:45:55: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:46:00: 1000000 INFO @ Sat, 15 Jan 2022 17:46:05: 2000000 INFO @ Sat, 15 Jan 2022 17:46:11: 3000000 INFO @ Sat, 15 Jan 2022 17:46:16: 4000000 INFO @ Sat, 15 Jan 2022 17:46:21: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:46:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:46:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:46:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:46:27: 6000000 INFO @ Sat, 15 Jan 2022 17:46:32: 7000000 INFO @ Sat, 15 Jan 2022 17:46:33: 1000000 INFO @ Sat, 15 Jan 2022 17:46:38: 8000000 INFO @ Sat, 15 Jan 2022 17:46:39: 2000000 INFO @ Sat, 15 Jan 2022 17:46:44: 9000000 INFO @ Sat, 15 Jan 2022 17:46:45: 3000000 INFO @ Sat, 15 Jan 2022 17:46:50: 10000000 INFO @ Sat, 15 Jan 2022 17:46:52: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:46:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:46:55: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:46:55: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:46:55: 11000000 INFO @ Sat, 15 Jan 2022 17:46:58: 5000000 INFO @ Sat, 15 Jan 2022 17:47:01: 12000000 INFO @ Sat, 15 Jan 2022 17:47:02: 1000000 INFO @ Sat, 15 Jan 2022 17:47:04: 6000000 INFO @ Sat, 15 Jan 2022 17:47:07: 13000000 INFO @ Sat, 15 Jan 2022 17:47:08: 2000000 INFO @ Sat, 15 Jan 2022 17:47:11: 7000000 INFO @ Sat, 15 Jan 2022 17:47:14: 14000000 INFO @ Sat, 15 Jan 2022 17:47:14: 3000000 INFO @ Sat, 15 Jan 2022 17:47:17: 8000000 INFO @ Sat, 15 Jan 2022 17:47:20: 15000000 INFO @ Sat, 15 Jan 2022 17:47:21: 4000000 INFO @ Sat, 15 Jan 2022 17:47:23: 9000000 INFO @ Sat, 15 Jan 2022 17:47:26: 16000000 INFO @ Sat, 15 Jan 2022 17:47:27: 5000000 INFO @ Sat, 15 Jan 2022 17:47:30: 10000000 INFO @ Sat, 15 Jan 2022 17:47:32: 17000000 INFO @ Sat, 15 Jan 2022 17:47:34: 6000000 INFO @ Sat, 15 Jan 2022 17:47:36: 11000000 INFO @ Sat, 15 Jan 2022 17:47:38: 18000000 INFO @ Sat, 15 Jan 2022 17:47:40: 7000000 INFO @ Sat, 15 Jan 2022 17:47:42: 12000000 INFO @ Sat, 15 Jan 2022 17:47:44: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:47:44: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:47:44: #1 total tags in treatment: 6990410 INFO @ Sat, 15 Jan 2022 17:47:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:47:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:47:45: #1 tags after filtering in treatment: 5748798 INFO @ Sat, 15 Jan 2022 17:47:45: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 17:47:45: #1 finished! INFO @ Sat, 15 Jan 2022 17:47:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:47:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:47:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:47:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:47:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:47:47: 8000000 INFO @ Sat, 15 Jan 2022 17:47:48: 13000000 INFO @ Sat, 15 Jan 2022 17:47:53: 9000000 INFO @ Sat, 15 Jan 2022 17:47:54: 14000000 INFO @ Sat, 15 Jan 2022 17:47:59: 10000000 INFO @ Sat, 15 Jan 2022 17:47:59: 15000000 INFO @ Sat, 15 Jan 2022 17:48:05: 16000000 INFO @ Sat, 15 Jan 2022 17:48:05: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:48:11: 17000000 INFO @ Sat, 15 Jan 2022 17:48:12: 12000000 INFO @ Sat, 15 Jan 2022 17:48:17: 18000000 INFO @ Sat, 15 Jan 2022 17:48:18: 13000000 INFO @ Sat, 15 Jan 2022 17:48:22: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:48:22: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:48:22: #1 total tags in treatment: 6990410 INFO @ Sat, 15 Jan 2022 17:48:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:48:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:48:22: #1 tags after filtering in treatment: 5748798 INFO @ Sat, 15 Jan 2022 17:48:22: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 17:48:22: #1 finished! INFO @ Sat, 15 Jan 2022 17:48:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:48:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:48:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:48:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:48:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:48:24: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:48:31: 15000000 INFO @ Sat, 15 Jan 2022 17:48:37: 16000000 INFO @ Sat, 15 Jan 2022 17:48:43: 17000000 INFO @ Sat, 15 Jan 2022 17:48:50: 18000000 INFO @ Sat, 15 Jan 2022 17:48:56: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:48:56: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:48:56: #1 total tags in treatment: 6990410 INFO @ Sat, 15 Jan 2022 17:48:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:48:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:48:56: #1 tags after filtering in treatment: 5748798 INFO @ Sat, 15 Jan 2022 17:48:56: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Jan 2022 17:48:56: #1 finished! INFO @ Sat, 15 Jan 2022 17:48:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:48:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:48:57: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:48:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:48:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786333/SRX9786333.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling