Job ID = 14519694 SRX = SRX9786332 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15613777 spots for SRR13362164/SRR13362164.sra Written 15613777 spots for SRR13362164/SRR13362164.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:29 15613777 reads; of these: 15613777 (100.00%) were paired; of these: 6733622 (43.13%) aligned concordantly 0 times 8202856 (52.54%) aligned concordantly exactly 1 time 677299 (4.34%) aligned concordantly >1 times ---- 6733622 pairs aligned concordantly 0 times; of these: 218657 (3.25%) aligned discordantly 1 time ---- 6514965 pairs aligned 0 times concordantly or discordantly; of these: 13029930 mates make up the pairs; of these: 7347950 (56.39%) aligned 0 times 5149942 (39.52%) aligned exactly 1 time 532038 (4.08%) aligned >1 times 76.47% overall alignment rate Time searching: 00:11:29 Overall time: 00:11:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 138730 / 9097756 = 0.0152 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:48:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:48:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:48:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:48:35: 1000000 INFO @ Sat, 15 Jan 2022 17:48:41: 2000000 INFO @ Sat, 15 Jan 2022 17:48:48: 3000000 INFO @ Sat, 15 Jan 2022 17:48:54: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:48:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:48:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:48:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:49:01: 5000000 INFO @ Sat, 15 Jan 2022 17:49:05: 1000000 INFO @ Sat, 15 Jan 2022 17:49:08: 6000000 INFO @ Sat, 15 Jan 2022 17:49:12: 2000000 INFO @ Sat, 15 Jan 2022 17:49:15: 7000000 INFO @ Sat, 15 Jan 2022 17:49:19: 3000000 INFO @ Sat, 15 Jan 2022 17:49:22: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:49:26: 4000000 INFO @ Sat, 15 Jan 2022 17:49:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:49:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:49:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:49:29: 9000000 INFO @ Sat, 15 Jan 2022 17:49:33: 5000000 INFO @ Sat, 15 Jan 2022 17:49:35: 1000000 INFO @ Sat, 15 Jan 2022 17:49:37: 10000000 INFO @ Sat, 15 Jan 2022 17:49:41: 6000000 INFO @ Sat, 15 Jan 2022 17:49:43: 2000000 INFO @ Sat, 15 Jan 2022 17:49:45: 11000000 INFO @ Sat, 15 Jan 2022 17:49:49: 7000000 INFO @ Sat, 15 Jan 2022 17:49:51: 3000000 INFO @ Sat, 15 Jan 2022 17:49:52: 12000000 INFO @ Sat, 15 Jan 2022 17:49:56: 8000000 INFO @ Sat, 15 Jan 2022 17:49:58: 4000000 INFO @ Sat, 15 Jan 2022 17:50:00: 13000000 INFO @ Sat, 15 Jan 2022 17:50:04: 9000000 INFO @ Sat, 15 Jan 2022 17:50:06: 5000000 INFO @ Sat, 15 Jan 2022 17:50:08: 14000000 INFO @ Sat, 15 Jan 2022 17:50:12: 10000000 INFO @ Sat, 15 Jan 2022 17:50:14: 6000000 INFO @ Sat, 15 Jan 2022 17:50:15: 15000000 INFO @ Sat, 15 Jan 2022 17:50:20: 11000000 INFO @ Sat, 15 Jan 2022 17:50:23: 16000000 INFO @ Sat, 15 Jan 2022 17:50:23: 7000000 INFO @ Sat, 15 Jan 2022 17:50:29: 12000000 INFO @ Sat, 15 Jan 2022 17:50:30: 17000000 INFO @ Sat, 15 Jan 2022 17:50:31: 8000000 INFO @ Sat, 15 Jan 2022 17:50:37: 13000000 INFO @ Sat, 15 Jan 2022 17:50:37: 18000000 INFO @ Sat, 15 Jan 2022 17:50:40: 9000000 INFO @ Sat, 15 Jan 2022 17:50:45: 19000000 INFO @ Sat, 15 Jan 2022 17:50:46: 14000000 INFO @ Sat, 15 Jan 2022 17:50:48: 10000000 INFO @ Sat, 15 Jan 2022 17:50:52: 20000000 INFO @ Sat, 15 Jan 2022 17:50:54: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:50:56: 11000000 INFO @ Sat, 15 Jan 2022 17:50:59: 21000000 INFO @ Sat, 15 Jan 2022 17:51:03: 16000000 INFO @ Sat, 15 Jan 2022 17:51:05: 12000000 INFO @ Sat, 15 Jan 2022 17:51:07: 22000000 INFO @ Sat, 15 Jan 2022 17:51:11: 17000000 INFO @ Sat, 15 Jan 2022 17:51:13: 13000000 INFO @ Sat, 15 Jan 2022 17:51:14: 23000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:51:19: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:51:19: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:51:19: #1 total tags in treatment: 8742191 INFO @ Sat, 15 Jan 2022 17:51:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:51:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:51:19: #1 tags after filtering in treatment: 6874417 INFO @ Sat, 15 Jan 2022 17:51:19: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 17:51:19: #1 finished! INFO @ Sat, 15 Jan 2022 17:51:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:51:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:51:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:51:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:51:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:51:20: 18000000 INFO @ Sat, 15 Jan 2022 17:51:21: 14000000 INFO @ Sat, 15 Jan 2022 17:51:27: 19000000 INFO @ Sat, 15 Jan 2022 17:51:29: 15000000 INFO @ Sat, 15 Jan 2022 17:51:34: 20000000 INFO @ Sat, 15 Jan 2022 17:51:37: 16000000 INFO @ Sat, 15 Jan 2022 17:51:41: 21000000 INFO @ Sat, 15 Jan 2022 17:51:45: 17000000 INFO @ Sat, 15 Jan 2022 17:51:48: 22000000 INFO @ Sat, 15 Jan 2022 17:51:53: 18000000 INFO @ Sat, 15 Jan 2022 17:51:54: 23000000 INFO @ Sat, 15 Jan 2022 17:51:58: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:51:58: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:51:58: #1 total tags in treatment: 8742191 INFO @ Sat, 15 Jan 2022 17:51:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:51:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:51:59: #1 tags after filtering in treatment: 6874417 INFO @ Sat, 15 Jan 2022 17:51:59: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 17:51:59: #1 finished! INFO @ Sat, 15 Jan 2022 17:51:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:51:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:51:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:51:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:51:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:52:00: 19000000 INFO @ Sat, 15 Jan 2022 17:52:07: 20000000 INFO @ Sat, 15 Jan 2022 17:52:13: 21000000 INFO @ Sat, 15 Jan 2022 17:52:19: 22000000 INFO @ Sat, 15 Jan 2022 17:52:25: 23000000 INFO @ Sat, 15 Jan 2022 17:52:29: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:52:29: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:52:29: #1 total tags in treatment: 8742191 INFO @ Sat, 15 Jan 2022 17:52:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:52:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:52:29: #1 tags after filtering in treatment: 6874417 INFO @ Sat, 15 Jan 2022 17:52:29: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 17:52:29: #1 finished! INFO @ Sat, 15 Jan 2022 17:52:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:52:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:52:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:52:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:52:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786332/SRX9786332.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling