Job ID = 14519693 SRX = SRX9786331 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16439262 spots for SRR13362163/SRR13362163.sra Written 16439262 spots for SRR13362163/SRR13362163.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:56 16439262 reads; of these: 16439262 (100.00%) were paired; of these: 6526286 (39.70%) aligned concordantly 0 times 9042671 (55.01%) aligned concordantly exactly 1 time 870305 (5.29%) aligned concordantly >1 times ---- 6526286 pairs aligned concordantly 0 times; of these: 200917 (3.08%) aligned discordantly 1 time ---- 6325369 pairs aligned 0 times concordantly or discordantly; of these: 12650738 mates make up the pairs; of these: 7131454 (56.37%) aligned 0 times 4940542 (39.05%) aligned exactly 1 time 578742 (4.57%) aligned >1 times 78.31% overall alignment rate Time searching: 00:08:57 Overall time: 00:08:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 235769 / 10112814 = 0.0233 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:42:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:42:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:42:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:43:03: 1000000 INFO @ Sat, 15 Jan 2022 17:43:08: 2000000 INFO @ Sat, 15 Jan 2022 17:43:12: 3000000 INFO @ Sat, 15 Jan 2022 17:43:17: 4000000 INFO @ Sat, 15 Jan 2022 17:43:21: 5000000 INFO @ Sat, 15 Jan 2022 17:43:26: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:43:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:43:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:43:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:43:31: 7000000 INFO @ Sat, 15 Jan 2022 17:43:33: 1000000 INFO @ Sat, 15 Jan 2022 17:43:36: 8000000 INFO @ Sat, 15 Jan 2022 17:43:38: 2000000 INFO @ Sat, 15 Jan 2022 17:43:40: 9000000 INFO @ Sat, 15 Jan 2022 17:43:43: 3000000 INFO @ Sat, 15 Jan 2022 17:43:45: 10000000 INFO @ Sat, 15 Jan 2022 17:43:48: 4000000 INFO @ Sat, 15 Jan 2022 17:43:50: 11000000 INFO @ Sat, 15 Jan 2022 17:43:53: 5000000 INFO @ Sat, 15 Jan 2022 17:43:55: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:43:58: 6000000 INFO @ Sat, 15 Jan 2022 17:43:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:43:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:43:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:44:00: 13000000 INFO @ Sat, 15 Jan 2022 17:44:03: 7000000 INFO @ Sat, 15 Jan 2022 17:44:04: 1000000 INFO @ Sat, 15 Jan 2022 17:44:05: 14000000 INFO @ Sat, 15 Jan 2022 17:44:08: 8000000 INFO @ Sat, 15 Jan 2022 17:44:10: 2000000 INFO @ Sat, 15 Jan 2022 17:44:10: 15000000 INFO @ Sat, 15 Jan 2022 17:44:13: 9000000 INFO @ Sat, 15 Jan 2022 17:44:15: 16000000 INFO @ Sat, 15 Jan 2022 17:44:16: 3000000 INFO @ Sat, 15 Jan 2022 17:44:18: 10000000 INFO @ Sat, 15 Jan 2022 17:44:20: 17000000 INFO @ Sat, 15 Jan 2022 17:44:22: 4000000 INFO @ Sat, 15 Jan 2022 17:44:23: 11000000 INFO @ Sat, 15 Jan 2022 17:44:25: 18000000 INFO @ Sat, 15 Jan 2022 17:44:27: 5000000 INFO @ Sat, 15 Jan 2022 17:44:28: 12000000 INFO @ Sat, 15 Jan 2022 17:44:30: 19000000 INFO @ Sat, 15 Jan 2022 17:44:33: 13000000 INFO @ Sat, 15 Jan 2022 17:44:33: 6000000 INFO @ Sat, 15 Jan 2022 17:44:35: 20000000 INFO @ Sat, 15 Jan 2022 17:44:38: 14000000 INFO @ Sat, 15 Jan 2022 17:44:39: 7000000 INFO @ Sat, 15 Jan 2022 17:44:40: 21000000 INFO @ Sat, 15 Jan 2022 17:44:43: 15000000 INFO @ Sat, 15 Jan 2022 17:44:45: 8000000 INFO @ Sat, 15 Jan 2022 17:44:45: 22000000 INFO @ Sat, 15 Jan 2022 17:44:48: 16000000 INFO @ Sat, 15 Jan 2022 17:44:50: 23000000 INFO @ Sat, 15 Jan 2022 17:44:51: 9000000 INFO @ Sat, 15 Jan 2022 17:44:53: 17000000 INFO @ Sat, 15 Jan 2022 17:44:55: 24000000 INFO @ Sat, 15 Jan 2022 17:44:57: 10000000 INFO @ Sat, 15 Jan 2022 17:44:58: 18000000 INFO @ Sat, 15 Jan 2022 17:45:00: 25000000 INFO @ Sat, 15 Jan 2022 17:45:01: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:45:01: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:45:01: #1 total tags in treatment: 9678568 INFO @ Sat, 15 Jan 2022 17:45:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:45:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:45:02: #1 tags after filtering in treatment: 7435379 INFO @ Sat, 15 Jan 2022 17:45:02: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 17:45:02: #1 finished! INFO @ Sat, 15 Jan 2022 17:45:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:45:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:45:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:45:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:45:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:45:03: 19000000 INFO @ Sat, 15 Jan 2022 17:45:03: 11000000 INFO @ Sat, 15 Jan 2022 17:45:08: 20000000 INFO @ Sat, 15 Jan 2022 17:45:09: 12000000 INFO @ Sat, 15 Jan 2022 17:45:13: 21000000 INFO @ Sat, 15 Jan 2022 17:45:14: 13000000 INFO @ Sat, 15 Jan 2022 17:45:17: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:45:20: 14000000 INFO @ Sat, 15 Jan 2022 17:45:22: 23000000 INFO @ Sat, 15 Jan 2022 17:45:26: 15000000 INFO @ Sat, 15 Jan 2022 17:45:27: 24000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:45:32: 16000000 INFO @ Sat, 15 Jan 2022 17:45:32: 25000000 INFO @ Sat, 15 Jan 2022 17:45:34: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:45:34: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:45:34: #1 total tags in treatment: 9678568 INFO @ Sat, 15 Jan 2022 17:45:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:45:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:45:34: #1 tags after filtering in treatment: 7435379 INFO @ Sat, 15 Jan 2022 17:45:34: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 17:45:34: #1 finished! INFO @ Sat, 15 Jan 2022 17:45:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:45:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:45:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:45:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:45:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:45:38: 17000000 INFO @ Sat, 15 Jan 2022 17:45:43: 18000000 INFO @ Sat, 15 Jan 2022 17:45:49: 19000000 INFO @ Sat, 15 Jan 2022 17:45:54: 20000000 INFO @ Sat, 15 Jan 2022 17:46:00: 21000000 INFO @ Sat, 15 Jan 2022 17:46:06: 22000000 INFO @ Sat, 15 Jan 2022 17:46:11: 23000000 INFO @ Sat, 15 Jan 2022 17:46:17: 24000000 INFO @ Sat, 15 Jan 2022 17:46:22: 25000000 INFO @ Sat, 15 Jan 2022 17:46:24: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:46:24: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:46:24: #1 total tags in treatment: 9678568 INFO @ Sat, 15 Jan 2022 17:46:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:46:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:46:24: #1 tags after filtering in treatment: 7435379 INFO @ Sat, 15 Jan 2022 17:46:24: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 17:46:24: #1 finished! INFO @ Sat, 15 Jan 2022 17:46:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:46:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:46:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:46:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:46:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786331/SRX9786331.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling