Job ID = 14519692 SRX = SRX9786330 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15576365 spots for SRR13362162/SRR13362162.sra Written 15576365 spots for SRR13362162/SRR13362162.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:41 15576365 reads; of these: 15576365 (100.00%) were paired; of these: 6348410 (40.76%) aligned concordantly 0 times 8497454 (54.55%) aligned concordantly exactly 1 time 730501 (4.69%) aligned concordantly >1 times ---- 6348410 pairs aligned concordantly 0 times; of these: 241377 (3.80%) aligned discordantly 1 time ---- 6107033 pairs aligned 0 times concordantly or discordantly; of these: 12214066 mates make up the pairs; of these: 6887084 (56.39%) aligned 0 times 4802297 (39.32%) aligned exactly 1 time 524685 (4.30%) aligned >1 times 77.89% overall alignment rate Time searching: 00:08:41 Overall time: 00:08:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 164172 / 9468275 = 0.0173 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:42:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:42:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:42:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:42:14: 1000000 INFO @ Sat, 15 Jan 2022 17:42:19: 2000000 INFO @ Sat, 15 Jan 2022 17:42:25: 3000000 INFO @ Sat, 15 Jan 2022 17:42:30: 4000000 INFO @ Sat, 15 Jan 2022 17:42:36: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:42:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:42:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:42:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:42:42: 6000000 INFO @ Sat, 15 Jan 2022 17:42:44: 1000000 INFO @ Sat, 15 Jan 2022 17:42:48: 7000000 INFO @ Sat, 15 Jan 2022 17:42:50: 2000000 INFO @ Sat, 15 Jan 2022 17:42:54: 8000000 INFO @ Sat, 15 Jan 2022 17:42:56: 3000000 INFO @ Sat, 15 Jan 2022 17:43:00: 9000000 INFO @ Sat, 15 Jan 2022 17:43:02: 4000000 INFO @ Sat, 15 Jan 2022 17:43:06: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:43:08: 5000000 INFO @ Sat, 15 Jan 2022 17:43:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:43:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:43:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:43:12: 11000000 INFO @ Sat, 15 Jan 2022 17:43:14: 1000000 INFO @ Sat, 15 Jan 2022 17:43:14: 6000000 INFO @ Sat, 15 Jan 2022 17:43:18: 12000000 INFO @ Sat, 15 Jan 2022 17:43:20: 2000000 INFO @ Sat, 15 Jan 2022 17:43:20: 7000000 INFO @ Sat, 15 Jan 2022 17:43:24: 13000000 INFO @ Sat, 15 Jan 2022 17:43:26: 3000000 INFO @ Sat, 15 Jan 2022 17:43:26: 8000000 INFO @ Sat, 15 Jan 2022 17:43:30: 14000000 INFO @ Sat, 15 Jan 2022 17:43:31: 4000000 INFO @ Sat, 15 Jan 2022 17:43:32: 9000000 INFO @ Sat, 15 Jan 2022 17:43:36: 15000000 INFO @ Sat, 15 Jan 2022 17:43:37: 5000000 INFO @ Sat, 15 Jan 2022 17:43:39: 10000000 INFO @ Sat, 15 Jan 2022 17:43:42: 6000000 INFO @ Sat, 15 Jan 2022 17:43:42: 16000000 INFO @ Sat, 15 Jan 2022 17:43:45: 11000000 INFO @ Sat, 15 Jan 2022 17:43:48: 7000000 INFO @ Sat, 15 Jan 2022 17:43:48: 17000000 INFO @ Sat, 15 Jan 2022 17:43:51: 12000000 INFO @ Sat, 15 Jan 2022 17:43:53: 8000000 INFO @ Sat, 15 Jan 2022 17:43:55: 18000000 INFO @ Sat, 15 Jan 2022 17:43:57: 13000000 INFO @ Sat, 15 Jan 2022 17:43:59: 9000000 INFO @ Sat, 15 Jan 2022 17:44:01: 19000000 INFO @ Sat, 15 Jan 2022 17:44:03: 14000000 INFO @ Sat, 15 Jan 2022 17:44:04: 10000000 INFO @ Sat, 15 Jan 2022 17:44:07: 20000000 INFO @ Sat, 15 Jan 2022 17:44:09: 15000000 INFO @ Sat, 15 Jan 2022 17:44:10: 11000000 INFO @ Sat, 15 Jan 2022 17:44:13: 21000000 INFO @ Sat, 15 Jan 2022 17:44:15: 16000000 INFO @ Sat, 15 Jan 2022 17:44:15: 12000000 INFO @ Sat, 15 Jan 2022 17:44:19: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:44:21: 13000000 INFO @ Sat, 15 Jan 2022 17:44:21: 17000000 INFO @ Sat, 15 Jan 2022 17:44:25: 23000000 INFO @ Sat, 15 Jan 2022 17:44:26: 14000000 INFO @ Sat, 15 Jan 2022 17:44:27: 18000000 INFO @ Sat, 15 Jan 2022 17:44:31: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:44:31: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:44:31: #1 total tags in treatment: 9064865 INFO @ Sat, 15 Jan 2022 17:44:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:44:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:44:31: #1 tags after filtering in treatment: 7069784 INFO @ Sat, 15 Jan 2022 17:44:31: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 15 Jan 2022 17:44:31: #1 finished! INFO @ Sat, 15 Jan 2022 17:44:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:44:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:44:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:44:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:44:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:44:32: 15000000 INFO @ Sat, 15 Jan 2022 17:44:33: 19000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:44:37: 16000000 INFO @ Sat, 15 Jan 2022 17:44:39: 20000000 INFO @ Sat, 15 Jan 2022 17:44:43: 17000000 INFO @ Sat, 15 Jan 2022 17:44:45: 21000000 INFO @ Sat, 15 Jan 2022 17:44:49: 18000000 INFO @ Sat, 15 Jan 2022 17:44:51: 22000000 INFO @ Sat, 15 Jan 2022 17:44:54: 19000000 INFO @ Sat, 15 Jan 2022 17:44:58: 23000000 INFO @ Sat, 15 Jan 2022 17:45:00: 20000000 INFO @ Sat, 15 Jan 2022 17:45:03: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:45:03: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:45:03: #1 total tags in treatment: 9064865 INFO @ Sat, 15 Jan 2022 17:45:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:45:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:45:04: #1 tags after filtering in treatment: 7069784 INFO @ Sat, 15 Jan 2022 17:45:04: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 15 Jan 2022 17:45:04: #1 finished! INFO @ Sat, 15 Jan 2022 17:45:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:45:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:45:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:45:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:45:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:45:05: 21000000 INFO @ Sat, 15 Jan 2022 17:45:10: 22000000 INFO @ Sat, 15 Jan 2022 17:45:15: 23000000 INFO @ Sat, 15 Jan 2022 17:45:20: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:45:20: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:45:20: #1 total tags in treatment: 9064865 INFO @ Sat, 15 Jan 2022 17:45:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:45:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:45:20: #1 tags after filtering in treatment: 7069784 INFO @ Sat, 15 Jan 2022 17:45:20: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 15 Jan 2022 17:45:20: #1 finished! INFO @ Sat, 15 Jan 2022 17:45:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:45:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:45:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:45:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:45:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786330/SRX9786330.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling