Job ID = 14519671 SRX = SRX9786329 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 13735295 spots for SRR13362161/SRR13362161.sra Written 13735295 spots for SRR13362161/SRR13362161.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:09 13735295 reads; of these: 13735295 (100.00%) were paired; of these: 5564845 (40.51%) aligned concordantly 0 times 7533882 (54.85%) aligned concordantly exactly 1 time 636568 (4.63%) aligned concordantly >1 times ---- 5564845 pairs aligned concordantly 0 times; of these: 228426 (4.10%) aligned discordantly 1 time ---- 5336419 pairs aligned 0 times concordantly or discordantly; of these: 10672838 mates make up the pairs; of these: 6116741 (57.31%) aligned 0 times 4107521 (38.49%) aligned exactly 1 time 448576 (4.20%) aligned >1 times 77.73% overall alignment rate Time searching: 00:10:09 Overall time: 00:10:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 195932 / 8397908 = 0.0233 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:38:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:38:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:38:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:38:13: 1000000 INFO @ Sat, 15 Jan 2022 17:38:18: 2000000 INFO @ Sat, 15 Jan 2022 17:38:23: 3000000 INFO @ Sat, 15 Jan 2022 17:38:29: 4000000 INFO @ Sat, 15 Jan 2022 17:38:34: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:38:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:38:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:38:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:38:40: 6000000 INFO @ Sat, 15 Jan 2022 17:38:43: 1000000 INFO @ Sat, 15 Jan 2022 17:38:46: 7000000 INFO @ Sat, 15 Jan 2022 17:38:50: 2000000 INFO @ Sat, 15 Jan 2022 17:38:52: 8000000 INFO @ Sat, 15 Jan 2022 17:38:56: 3000000 INFO @ Sat, 15 Jan 2022 17:38:58: 9000000 INFO @ Sat, 15 Jan 2022 17:39:02: 4000000 INFO @ Sat, 15 Jan 2022 17:39:05: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:39:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:39:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:39:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:39:08: 5000000 INFO @ Sat, 15 Jan 2022 17:39:11: 11000000 INFO @ Sat, 15 Jan 2022 17:39:14: 1000000 INFO @ Sat, 15 Jan 2022 17:39:14: 6000000 INFO @ Sat, 15 Jan 2022 17:39:17: 12000000 INFO @ Sat, 15 Jan 2022 17:39:20: 2000000 INFO @ Sat, 15 Jan 2022 17:39:21: 7000000 INFO @ Sat, 15 Jan 2022 17:39:24: 13000000 INFO @ Sat, 15 Jan 2022 17:39:26: 3000000 INFO @ Sat, 15 Jan 2022 17:39:27: 8000000 INFO @ Sat, 15 Jan 2022 17:39:30: 14000000 INFO @ Sat, 15 Jan 2022 17:39:32: 4000000 INFO @ Sat, 15 Jan 2022 17:39:33: 9000000 INFO @ Sat, 15 Jan 2022 17:39:36: 15000000 INFO @ Sat, 15 Jan 2022 17:39:39: 5000000 INFO @ Sat, 15 Jan 2022 17:39:40: 10000000 INFO @ Sat, 15 Jan 2022 17:39:43: 16000000 INFO @ Sat, 15 Jan 2022 17:39:45: 6000000 INFO @ Sat, 15 Jan 2022 17:39:46: 11000000 INFO @ Sat, 15 Jan 2022 17:39:49: 17000000 INFO @ Sat, 15 Jan 2022 17:39:52: 7000000 INFO @ Sat, 15 Jan 2022 17:39:53: 12000000 INFO @ Sat, 15 Jan 2022 17:39:55: 18000000 INFO @ Sat, 15 Jan 2022 17:39:59: 8000000 INFO @ Sat, 15 Jan 2022 17:39:59: 13000000 INFO @ Sat, 15 Jan 2022 17:40:02: 19000000 INFO @ Sat, 15 Jan 2022 17:40:05: 9000000 INFO @ Sat, 15 Jan 2022 17:40:05: 14000000 INFO @ Sat, 15 Jan 2022 17:40:08: 20000000 INFO @ Sat, 15 Jan 2022 17:40:12: 15000000 INFO @ Sat, 15 Jan 2022 17:40:12: 10000000 INFO @ Sat, 15 Jan 2022 17:40:14: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:40:14: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:40:14: #1 total tags in treatment: 7975987 INFO @ Sat, 15 Jan 2022 17:40:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:40:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:40:15: #1 tags after filtering in treatment: 6362555 INFO @ Sat, 15 Jan 2022 17:40:15: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 17:40:15: #1 finished! INFO @ Sat, 15 Jan 2022 17:40:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:40:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:40:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:40:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:40:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:40:18: 16000000 INFO @ Sat, 15 Jan 2022 17:40:19: 11000000 INFO @ Sat, 15 Jan 2022 17:40:24: 17000000 INFO @ Sat, 15 Jan 2022 17:40:25: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:40:30: 18000000 INFO @ Sat, 15 Jan 2022 17:40:31: 13000000 INFO @ Sat, 15 Jan 2022 17:40:36: 19000000 INFO @ Sat, 15 Jan 2022 17:40:38: 14000000 INFO @ Sat, 15 Jan 2022 17:40:42: 20000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:40:44: 15000000 INFO @ Sat, 15 Jan 2022 17:40:48: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:40:48: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:40:48: #1 total tags in treatment: 7975987 INFO @ Sat, 15 Jan 2022 17:40:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:40:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:40:48: #1 tags after filtering in treatment: 6362555 INFO @ Sat, 15 Jan 2022 17:40:48: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 17:40:48: #1 finished! INFO @ Sat, 15 Jan 2022 17:40:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:40:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:40:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:40:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:40:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:40:49: 16000000 INFO @ Sat, 15 Jan 2022 17:40:55: 17000000 INFO @ Sat, 15 Jan 2022 17:41:00: 18000000 INFO @ Sat, 15 Jan 2022 17:41:06: 19000000 INFO @ Sat, 15 Jan 2022 17:41:11: 20000000 INFO @ Sat, 15 Jan 2022 17:41:17: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:41:17: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:41:17: #1 total tags in treatment: 7975987 INFO @ Sat, 15 Jan 2022 17:41:17: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:41:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:41:17: #1 tags after filtering in treatment: 6362555 INFO @ Sat, 15 Jan 2022 17:41:17: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 17:41:17: #1 finished! INFO @ Sat, 15 Jan 2022 17:41:17: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:41:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:41:17: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:41:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:41:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786329/SRX9786329.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling