Job ID = 14519670 SRX = SRX9786328 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 14841657 spots for SRR13362160/SRR13362160.sra Written 14841657 spots for SRR13362160/SRR13362160.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:40 14841657 reads; of these: 14841657 (100.00%) were paired; of these: 6343706 (42.74%) aligned concordantly 0 times 7836293 (52.80%) aligned concordantly exactly 1 time 661658 (4.46%) aligned concordantly >1 times ---- 6343706 pairs aligned concordantly 0 times; of these: 309227 (4.87%) aligned discordantly 1 time ---- 6034479 pairs aligned 0 times concordantly or discordantly; of these: 12068958 mates make up the pairs; of these: 6972905 (57.78%) aligned 0 times 4588308 (38.02%) aligned exactly 1 time 507745 (4.21%) aligned >1 times 76.51% overall alignment rate Time searching: 00:07:40 Overall time: 00:07:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 220843 / 8806052 = 0.0251 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:33:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:33:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:33:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:34:02: 1000000 INFO @ Sat, 15 Jan 2022 17:34:07: 2000000 INFO @ Sat, 15 Jan 2022 17:34:11: 3000000 INFO @ Sat, 15 Jan 2022 17:34:16: 4000000 INFO @ Sat, 15 Jan 2022 17:34:20: 5000000 INFO @ Sat, 15 Jan 2022 17:34:24: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:34:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:34:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:34:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:34:29: 7000000 INFO @ Sat, 15 Jan 2022 17:34:33: 1000000 INFO @ Sat, 15 Jan 2022 17:34:33: 8000000 INFO @ Sat, 15 Jan 2022 17:34:37: 2000000 INFO @ Sat, 15 Jan 2022 17:34:38: 9000000 INFO @ Sat, 15 Jan 2022 17:34:42: 3000000 INFO @ Sat, 15 Jan 2022 17:34:43: 10000000 INFO @ Sat, 15 Jan 2022 17:34:46: 4000000 INFO @ Sat, 15 Jan 2022 17:34:47: 11000000 INFO @ Sat, 15 Jan 2022 17:34:51: 5000000 INFO @ Sat, 15 Jan 2022 17:34:52: 12000000 INFO @ Sat, 15 Jan 2022 17:34:56: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:34:57: 13000000 INFO @ Sat, 15 Jan 2022 17:34:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:34:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:34:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:35:00: 7000000 INFO @ Sat, 15 Jan 2022 17:35:01: 14000000 INFO @ Sat, 15 Jan 2022 17:35:03: 1000000 INFO @ Sat, 15 Jan 2022 17:35:05: 8000000 INFO @ Sat, 15 Jan 2022 17:35:06: 15000000 INFO @ Sat, 15 Jan 2022 17:35:08: 2000000 INFO @ Sat, 15 Jan 2022 17:35:10: 9000000 INFO @ Sat, 15 Jan 2022 17:35:11: 16000000 INFO @ Sat, 15 Jan 2022 17:35:12: 3000000 INFO @ Sat, 15 Jan 2022 17:35:15: 10000000 INFO @ Sat, 15 Jan 2022 17:35:15: 17000000 INFO @ Sat, 15 Jan 2022 17:35:17: 4000000 INFO @ Sat, 15 Jan 2022 17:35:20: 11000000 INFO @ Sat, 15 Jan 2022 17:35:20: 18000000 INFO @ Sat, 15 Jan 2022 17:35:22: 5000000 INFO @ Sat, 15 Jan 2022 17:35:25: 12000000 INFO @ Sat, 15 Jan 2022 17:35:25: 19000000 INFO @ Sat, 15 Jan 2022 17:35:27: 6000000 INFO @ Sat, 15 Jan 2022 17:35:29: 13000000 INFO @ Sat, 15 Jan 2022 17:35:30: 20000000 INFO @ Sat, 15 Jan 2022 17:35:31: 7000000 INFO @ Sat, 15 Jan 2022 17:35:34: 14000000 INFO @ Sat, 15 Jan 2022 17:35:34: 21000000 INFO @ Sat, 15 Jan 2022 17:35:36: 8000000 INFO @ Sat, 15 Jan 2022 17:35:39: 15000000 INFO @ Sat, 15 Jan 2022 17:35:39: 22000000 INFO @ Sat, 15 Jan 2022 17:35:40: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:35:40: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:35:40: #1 total tags in treatment: 8279361 INFO @ Sat, 15 Jan 2022 17:35:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:35:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:35:40: #1 tags after filtering in treatment: 6524113 INFO @ Sat, 15 Jan 2022 17:35:40: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 17:35:40: #1 finished! INFO @ Sat, 15 Jan 2022 17:35:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:35:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:35:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:35:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:35:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:35:41: 9000000 INFO @ Sat, 15 Jan 2022 17:35:44: 16000000 INFO @ Sat, 15 Jan 2022 17:35:46: 10000000 INFO @ Sat, 15 Jan 2022 17:35:48: 17000000 INFO @ Sat, 15 Jan 2022 17:35:50: 11000000 INFO @ Sat, 15 Jan 2022 17:35:53: 18000000 INFO @ Sat, 15 Jan 2022 17:35:55: 12000000 INFO @ Sat, 15 Jan 2022 17:35:58: 19000000 INFO @ Sat, 15 Jan 2022 17:36:00: 13000000 INFO @ Sat, 15 Jan 2022 17:36:03: 20000000 INFO @ Sat, 15 Jan 2022 17:36:05: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:36:08: 21000000 INFO @ Sat, 15 Jan 2022 17:36:10: 15000000 INFO @ Sat, 15 Jan 2022 17:36:12: 22000000 INFO @ Sat, 15 Jan 2022 17:36:14: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:36:14: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:36:14: #1 total tags in treatment: 8279361 INFO @ Sat, 15 Jan 2022 17:36:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:36:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:36:14: #1 tags after filtering in treatment: 6524113 INFO @ Sat, 15 Jan 2022 17:36:14: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 17:36:14: #1 finished! INFO @ Sat, 15 Jan 2022 17:36:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:36:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:36:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:36:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:36:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:36:14: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:36:19: 17000000 INFO @ Sat, 15 Jan 2022 17:36:24: 18000000 INFO @ Sat, 15 Jan 2022 17:36:28: 19000000 INFO @ Sat, 15 Jan 2022 17:36:33: 20000000 INFO @ Sat, 15 Jan 2022 17:36:38: 21000000 INFO @ Sat, 15 Jan 2022 17:36:42: 22000000 INFO @ Sat, 15 Jan 2022 17:36:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:36:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:36:43: #1 total tags in treatment: 8279361 INFO @ Sat, 15 Jan 2022 17:36:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:36:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:36:44: #1 tags after filtering in treatment: 6524113 INFO @ Sat, 15 Jan 2022 17:36:44: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 17:36:44: #1 finished! INFO @ Sat, 15 Jan 2022 17:36:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:36:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:36:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:36:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:36:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786328/SRX9786328.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling