Job ID = 14519667 SRX = SRX9786327 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15777452 spots for SRR13362159/SRR13362159.sra Written 15777452 spots for SRR13362159/SRR13362159.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:21 15777452 reads; of these: 15777452 (100.00%) were paired; of these: 2218395 (14.06%) aligned concordantly 0 times 11950886 (75.75%) aligned concordantly exactly 1 time 1608171 (10.19%) aligned concordantly >1 times ---- 2218395 pairs aligned concordantly 0 times; of these: 118450 (5.34%) aligned discordantly 1 time ---- 2099945 pairs aligned 0 times concordantly or discordantly; of these: 4199890 mates make up the pairs; of these: 2575030 (61.31%) aligned 0 times 1389077 (33.07%) aligned exactly 1 time 235783 (5.61%) aligned >1 times 91.84% overall alignment rate Time searching: 00:12:21 Overall time: 00:12:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 784465 / 13675834 = 0.0574 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:41:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:41:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:41:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:41:14: 1000000 INFO @ Sat, 15 Jan 2022 17:41:20: 2000000 INFO @ Sat, 15 Jan 2022 17:41:26: 3000000 INFO @ Sat, 15 Jan 2022 17:41:31: 4000000 INFO @ Sat, 15 Jan 2022 17:41:36: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:41:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:41:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:41:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:41:42: 6000000 INFO @ Sat, 15 Jan 2022 17:41:45: 1000000 INFO @ Sat, 15 Jan 2022 17:41:48: 7000000 INFO @ Sat, 15 Jan 2022 17:41:52: 2000000 INFO @ Sat, 15 Jan 2022 17:41:55: 8000000 INFO @ Sat, 15 Jan 2022 17:41:58: 3000000 INFO @ Sat, 15 Jan 2022 17:42:01: 9000000 INFO @ Sat, 15 Jan 2022 17:42:04: 4000000 INFO @ Sat, 15 Jan 2022 17:42:07: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:42:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:42:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:42:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:42:11: 5000000 INFO @ Sat, 15 Jan 2022 17:42:13: 11000000 INFO @ Sat, 15 Jan 2022 17:42:15: 1000000 INFO @ Sat, 15 Jan 2022 17:42:17: 6000000 INFO @ Sat, 15 Jan 2022 17:42:19: 12000000 INFO @ Sat, 15 Jan 2022 17:42:22: 2000000 INFO @ Sat, 15 Jan 2022 17:42:23: 7000000 INFO @ Sat, 15 Jan 2022 17:42:26: 13000000 INFO @ Sat, 15 Jan 2022 17:42:28: 3000000 INFO @ Sat, 15 Jan 2022 17:42:29: 8000000 INFO @ Sat, 15 Jan 2022 17:42:32: 14000000 INFO @ Sat, 15 Jan 2022 17:42:35: 4000000 INFO @ Sat, 15 Jan 2022 17:42:36: 9000000 INFO @ Sat, 15 Jan 2022 17:42:38: 15000000 INFO @ Sat, 15 Jan 2022 17:42:41: 5000000 INFO @ Sat, 15 Jan 2022 17:42:43: 10000000 INFO @ Sat, 15 Jan 2022 17:42:44: 16000000 INFO @ Sat, 15 Jan 2022 17:42:47: 6000000 INFO @ Sat, 15 Jan 2022 17:42:49: 11000000 INFO @ Sat, 15 Jan 2022 17:42:51: 17000000 INFO @ Sat, 15 Jan 2022 17:42:54: 7000000 INFO @ Sat, 15 Jan 2022 17:42:55: 12000000 INFO @ Sat, 15 Jan 2022 17:42:57: 18000000 INFO @ Sat, 15 Jan 2022 17:43:00: 8000000 INFO @ Sat, 15 Jan 2022 17:43:02: 13000000 INFO @ Sat, 15 Jan 2022 17:43:03: 19000000 INFO @ Sat, 15 Jan 2022 17:43:06: 9000000 INFO @ Sat, 15 Jan 2022 17:43:08: 14000000 INFO @ Sat, 15 Jan 2022 17:43:10: 20000000 INFO @ Sat, 15 Jan 2022 17:43:12: 10000000 INFO @ Sat, 15 Jan 2022 17:43:14: 15000000 INFO @ Sat, 15 Jan 2022 17:43:16: 21000000 INFO @ Sat, 15 Jan 2022 17:43:19: 11000000 INFO @ Sat, 15 Jan 2022 17:43:21: 16000000 INFO @ Sat, 15 Jan 2022 17:43:23: 22000000 INFO @ Sat, 15 Jan 2022 17:43:25: 12000000 INFO @ Sat, 15 Jan 2022 17:43:27: 17000000 INFO @ Sat, 15 Jan 2022 17:43:29: 23000000 INFO @ Sat, 15 Jan 2022 17:43:31: 13000000 INFO @ Sat, 15 Jan 2022 17:43:33: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:43:36: 24000000 INFO @ Sat, 15 Jan 2022 17:43:38: 14000000 INFO @ Sat, 15 Jan 2022 17:43:40: 19000000 INFO @ Sat, 15 Jan 2022 17:43:43: 25000000 INFO @ Sat, 15 Jan 2022 17:43:45: 15000000 INFO @ Sat, 15 Jan 2022 17:43:47: 20000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:43:49: 26000000 INFO @ Sat, 15 Jan 2022 17:43:51: 16000000 INFO @ Sat, 15 Jan 2022 17:43:53: 21000000 INFO @ Sat, 15 Jan 2022 17:43:56: 27000000 INFO @ Sat, 15 Jan 2022 17:43:58: 17000000 INFO @ Sat, 15 Jan 2022 17:43:59: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:43:59: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:43:59: #1 total tags in treatment: 12777298 INFO @ Sat, 15 Jan 2022 17:43:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:43:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:43:59: #1 tags after filtering in treatment: 7900104 INFO @ Sat, 15 Jan 2022 17:43:59: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 17:43:59: #1 finished! INFO @ Sat, 15 Jan 2022 17:43:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:43:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:43:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:43:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:43:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:44:00: 22000000 INFO @ Sat, 15 Jan 2022 17:44:05: 18000000 INFO @ Sat, 15 Jan 2022 17:44:07: 23000000 INFO @ Sat, 15 Jan 2022 17:44:11: 19000000 INFO @ Sat, 15 Jan 2022 17:44:13: 24000000 INFO @ Sat, 15 Jan 2022 17:44:18: 20000000 INFO @ Sat, 15 Jan 2022 17:44:20: 25000000 INFO @ Sat, 15 Jan 2022 17:44:24: 21000000 INFO @ Sat, 15 Jan 2022 17:44:26: 26000000 INFO @ Sat, 15 Jan 2022 17:44:31: 22000000 INFO @ Sat, 15 Jan 2022 17:44:33: 27000000 INFO @ Sat, 15 Jan 2022 17:44:36: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:44:36: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:44:36: #1 total tags in treatment: 12777298 INFO @ Sat, 15 Jan 2022 17:44:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:44:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:44:36: #1 tags after filtering in treatment: 7900104 INFO @ Sat, 15 Jan 2022 17:44:36: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 17:44:36: #1 finished! INFO @ Sat, 15 Jan 2022 17:44:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:44:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:44:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:44:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:44:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:44:38: 23000000 INFO @ Sat, 15 Jan 2022 17:44:44: 24000000 INFO @ Sat, 15 Jan 2022 17:44:50: 25000000 INFO @ Sat, 15 Jan 2022 17:44:56: 26000000 INFO @ Sat, 15 Jan 2022 17:45:02: 27000000 INFO @ Sat, 15 Jan 2022 17:45:04: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:45:04: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:45:04: #1 total tags in treatment: 12777298 INFO @ Sat, 15 Jan 2022 17:45:04: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:45:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:45:04: #1 tags after filtering in treatment: 7900104 INFO @ Sat, 15 Jan 2022 17:45:04: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 17:45:04: #1 finished! INFO @ Sat, 15 Jan 2022 17:45:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:45:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:45:05: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:45:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:45:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786327/SRX9786327.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling