Job ID = 14519666 SRX = SRX9786326 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9422222 spots for SRR13362158/SRR13362158.sra Written 9422222 spots for SRR13362158/SRR13362158.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:55 9422222 reads; of these: 9422222 (100.00%) were paired; of these: 1500070 (15.92%) aligned concordantly 0 times 7116604 (75.53%) aligned concordantly exactly 1 time 805548 (8.55%) aligned concordantly >1 times ---- 1500070 pairs aligned concordantly 0 times; of these: 134405 (8.96%) aligned discordantly 1 time ---- 1365665 pairs aligned 0 times concordantly or discordantly; of these: 2731330 mates make up the pairs; of these: 1645384 (60.24%) aligned 0 times 933677 (34.18%) aligned exactly 1 time 152269 (5.57%) aligned >1 times 91.27% overall alignment rate Time searching: 00:07:55 Overall time: 00:07:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 364223 / 8055740 = 0.0452 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:32:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:32:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:32:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:32:20: 1000000 INFO @ Sat, 15 Jan 2022 17:32:27: 2000000 INFO @ Sat, 15 Jan 2022 17:32:35: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:32:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:32:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:32:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:32:42: 4000000 INFO @ Sat, 15 Jan 2022 17:32:50: 1000000 INFO @ Sat, 15 Jan 2022 17:32:51: 5000000 INFO @ Sat, 15 Jan 2022 17:32:59: 2000000 INFO @ Sat, 15 Jan 2022 17:32:59: 6000000 INFO @ Sat, 15 Jan 2022 17:33:07: 3000000 INFO @ Sat, 15 Jan 2022 17:33:08: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:33:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:33:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:33:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:33:15: 4000000 INFO @ Sat, 15 Jan 2022 17:33:17: 8000000 INFO @ Sat, 15 Jan 2022 17:33:20: 1000000 INFO @ Sat, 15 Jan 2022 17:33:23: 5000000 INFO @ Sat, 15 Jan 2022 17:33:26: 9000000 INFO @ Sat, 15 Jan 2022 17:33:28: 2000000 INFO @ Sat, 15 Jan 2022 17:33:32: 6000000 INFO @ Sat, 15 Jan 2022 17:33:35: 10000000 INFO @ Sat, 15 Jan 2022 17:33:36: 3000000 INFO @ Sat, 15 Jan 2022 17:33:41: 7000000 INFO @ Sat, 15 Jan 2022 17:33:44: 11000000 INFO @ Sat, 15 Jan 2022 17:33:44: 4000000 INFO @ Sat, 15 Jan 2022 17:33:49: 8000000 INFO @ Sat, 15 Jan 2022 17:33:52: 5000000 INFO @ Sat, 15 Jan 2022 17:33:53: 12000000 INFO @ Sat, 15 Jan 2022 17:33:57: 9000000 INFO @ Sat, 15 Jan 2022 17:34:00: 6000000 INFO @ Sat, 15 Jan 2022 17:34:02: 13000000 INFO @ Sat, 15 Jan 2022 17:34:05: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:34:08: 7000000 INFO @ Sat, 15 Jan 2022 17:34:11: 14000000 INFO @ Sat, 15 Jan 2022 17:34:14: 11000000 INFO @ Sat, 15 Jan 2022 17:34:17: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:34:20: 15000000 INFO @ Sat, 15 Jan 2022 17:34:23: 12000000 INFO @ Sat, 15 Jan 2022 17:34:25: 9000000 INFO @ Sat, 15 Jan 2022 17:34:29: 16000000 INFO @ Sat, 15 Jan 2022 17:34:31: 13000000 INFO @ Sat, 15 Jan 2022 17:34:33: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:34:33: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:34:33: #1 total tags in treatment: 7560646 INFO @ Sat, 15 Jan 2022 17:34:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:34:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:34:33: 10000000 INFO @ Sat, 15 Jan 2022 17:34:33: #1 tags after filtering in treatment: 4944615 INFO @ Sat, 15 Jan 2022 17:34:33: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 17:34:33: #1 finished! INFO @ Sat, 15 Jan 2022 17:34:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:34:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:34:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:34:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:34:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:34:39: 14000000 INFO @ Sat, 15 Jan 2022 17:34:41: 11000000 INFO @ Sat, 15 Jan 2022 17:34:47: 15000000 INFO @ Sat, 15 Jan 2022 17:34:49: 12000000 INFO @ Sat, 15 Jan 2022 17:34:55: 16000000 INFO @ Sat, 15 Jan 2022 17:34:57: 13000000 INFO @ Sat, 15 Jan 2022 17:34:59: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:34:59: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:34:59: #1 total tags in treatment: 7560646 INFO @ Sat, 15 Jan 2022 17:34:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:34:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:34:59: #1 tags after filtering in treatment: 4944615 INFO @ Sat, 15 Jan 2022 17:34:59: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 17:34:59: #1 finished! INFO @ Sat, 15 Jan 2022 17:34:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:34:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:35:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:35:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:35:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:35:04: 14000000 INFO @ Sat, 15 Jan 2022 17:35:10: 15000000 INFO @ Sat, 15 Jan 2022 17:35:16: 16000000 INFO @ Sat, 15 Jan 2022 17:35:19: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:35:19: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:35:19: #1 total tags in treatment: 7560646 INFO @ Sat, 15 Jan 2022 17:35:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:35:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:35:19: #1 tags after filtering in treatment: 4944615 INFO @ Sat, 15 Jan 2022 17:35:19: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 17:35:19: #1 finished! INFO @ Sat, 15 Jan 2022 17:35:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:35:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:35:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:35:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:35:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786326/SRX9786326.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling