Job ID = 14519665 SRX = SRX9786325 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10034502 spots for SRR13362157/SRR13362157.sra Written 10034502 spots for SRR13362157/SRR13362157.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:55 10034502 reads; of these: 10034502 (100.00%) were paired; of these: 1656033 (16.50%) aligned concordantly 0 times 7542415 (75.16%) aligned concordantly exactly 1 time 836054 (8.33%) aligned concordantly >1 times ---- 1656033 pairs aligned concordantly 0 times; of these: 137512 (8.30%) aligned discordantly 1 time ---- 1518521 pairs aligned 0 times concordantly or discordantly; of these: 3037042 mates make up the pairs; of these: 1939491 (63.86%) aligned 0 times 943101 (31.05%) aligned exactly 1 time 154450 (5.09%) aligned >1 times 90.34% overall alignment rate Time searching: 00:08:55 Overall time: 00:08:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 493129 / 8514975 = 0.0579 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:33:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:33:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:33:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:34:00: 1000000 INFO @ Sat, 15 Jan 2022 17:34:06: 2000000 INFO @ Sat, 15 Jan 2022 17:34:11: 3000000 INFO @ Sat, 15 Jan 2022 17:34:17: 4000000 INFO @ Sat, 15 Jan 2022 17:34:23: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:34:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:34:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:34:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:34:28: 6000000 INFO @ Sat, 15 Jan 2022 17:34:31: 1000000 INFO @ Sat, 15 Jan 2022 17:34:35: 7000000 INFO @ Sat, 15 Jan 2022 17:34:37: 2000000 INFO @ Sat, 15 Jan 2022 17:34:41: 8000000 INFO @ Sat, 15 Jan 2022 17:34:44: 3000000 INFO @ Sat, 15 Jan 2022 17:34:48: 9000000 INFO @ Sat, 15 Jan 2022 17:34:50: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:34:54: 10000000 INFO @ Sat, 15 Jan 2022 17:34:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:34:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:34:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:34:57: 5000000 INFO @ Sat, 15 Jan 2022 17:35:00: 11000000 INFO @ Sat, 15 Jan 2022 17:35:01: 1000000 INFO @ Sat, 15 Jan 2022 17:35:03: 6000000 INFO @ Sat, 15 Jan 2022 17:35:07: 12000000 INFO @ Sat, 15 Jan 2022 17:35:07: 2000000 INFO @ Sat, 15 Jan 2022 17:35:09: 7000000 INFO @ Sat, 15 Jan 2022 17:35:13: 13000000 INFO @ Sat, 15 Jan 2022 17:35:14: 3000000 INFO @ Sat, 15 Jan 2022 17:35:16: 8000000 INFO @ Sat, 15 Jan 2022 17:35:20: 14000000 INFO @ Sat, 15 Jan 2022 17:35:20: 4000000 INFO @ Sat, 15 Jan 2022 17:35:22: 9000000 INFO @ Sat, 15 Jan 2022 17:35:26: 15000000 INFO @ Sat, 15 Jan 2022 17:35:27: 5000000 INFO @ Sat, 15 Jan 2022 17:35:29: 10000000 INFO @ Sat, 15 Jan 2022 17:35:33: 16000000 INFO @ Sat, 15 Jan 2022 17:35:33: 6000000 INFO @ Sat, 15 Jan 2022 17:35:35: 11000000 INFO @ Sat, 15 Jan 2022 17:35:39: 17000000 INFO @ Sat, 15 Jan 2022 17:35:40: 7000000 INFO @ Sat, 15 Jan 2022 17:35:40: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:35:40: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:35:40: #1 total tags in treatment: 7888608 INFO @ Sat, 15 Jan 2022 17:35:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:35:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:35:40: #1 tags after filtering in treatment: 5233141 INFO @ Sat, 15 Jan 2022 17:35:40: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 17:35:40: #1 finished! INFO @ Sat, 15 Jan 2022 17:35:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:35:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:35:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:35:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:35:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:35:42: 12000000 INFO @ Sat, 15 Jan 2022 17:35:46: 8000000 INFO @ Sat, 15 Jan 2022 17:35:48: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:35:52: 9000000 INFO @ Sat, 15 Jan 2022 17:35:54: 14000000 INFO @ Sat, 15 Jan 2022 17:35:59: 10000000 INFO @ Sat, 15 Jan 2022 17:36:01: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:36:05: 11000000 INFO @ Sat, 15 Jan 2022 17:36:07: 16000000 INFO @ Sat, 15 Jan 2022 17:36:12: 12000000 INFO @ Sat, 15 Jan 2022 17:36:14: 17000000 INFO @ Sat, 15 Jan 2022 17:36:15: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:36:15: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:36:15: #1 total tags in treatment: 7888608 INFO @ Sat, 15 Jan 2022 17:36:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:36:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:36:15: #1 tags after filtering in treatment: 5233141 INFO @ Sat, 15 Jan 2022 17:36:15: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 17:36:15: #1 finished! INFO @ Sat, 15 Jan 2022 17:36:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:36:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:36:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:36:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:36:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:36:18: 13000000 INFO @ Sat, 15 Jan 2022 17:36:24: 14000000 INFO @ Sat, 15 Jan 2022 17:36:29: 15000000 INFO @ Sat, 15 Jan 2022 17:36:35: 16000000 INFO @ Sat, 15 Jan 2022 17:36:41: 17000000 INFO @ Sat, 15 Jan 2022 17:36:41: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:36:41: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:36:41: #1 total tags in treatment: 7888608 INFO @ Sat, 15 Jan 2022 17:36:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:36:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:36:42: #1 tags after filtering in treatment: 5233141 INFO @ Sat, 15 Jan 2022 17:36:42: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 17:36:42: #1 finished! INFO @ Sat, 15 Jan 2022 17:36:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:36:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:36:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:36:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:36:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786325/SRX9786325.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling