Job ID = 14519663 SRX = SRX9786323 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16153968 spots for SRR13362155/SRR13362155.sra Written 16153968 spots for SRR13362155/SRR13362155.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:31 16153968 reads; of these: 16153968 (100.00%) were paired; of these: 2762578 (17.10%) aligned concordantly 0 times 11795225 (73.02%) aligned concordantly exactly 1 time 1596165 (9.88%) aligned concordantly >1 times ---- 2762578 pairs aligned concordantly 0 times; of these: 141705 (5.13%) aligned discordantly 1 time ---- 2620873 pairs aligned 0 times concordantly or discordantly; of these: 5241746 mates make up the pairs; of these: 3004067 (57.31%) aligned 0 times 1912419 (36.48%) aligned exactly 1 time 325260 (6.21%) aligned >1 times 90.70% overall alignment rate Time searching: 00:12:31 Overall time: 00:12:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 663238 / 13531375 = 0.0490 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:40:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:40:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:40:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:40:57: 1000000 INFO @ Sat, 15 Jan 2022 17:41:04: 2000000 INFO @ Sat, 15 Jan 2022 17:41:10: 3000000 INFO @ Sat, 15 Jan 2022 17:41:16: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:41:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:41:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:41:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:41:22: 5000000 INFO @ Sat, 15 Jan 2022 17:41:27: 1000000 INFO @ Sat, 15 Jan 2022 17:41:29: 6000000 INFO @ Sat, 15 Jan 2022 17:41:34: 2000000 INFO @ Sat, 15 Jan 2022 17:41:35: 7000000 INFO @ Sat, 15 Jan 2022 17:41:40: 3000000 INFO @ Sat, 15 Jan 2022 17:41:42: 8000000 INFO @ Sat, 15 Jan 2022 17:41:47: 4000000 INFO @ Sat, 15 Jan 2022 17:41:48: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:41:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:41:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:41:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:41:54: 5000000 INFO @ Sat, 15 Jan 2022 17:41:55: 10000000 INFO @ Sat, 15 Jan 2022 17:41:57: 1000000 INFO @ Sat, 15 Jan 2022 17:42:00: 6000000 INFO @ Sat, 15 Jan 2022 17:42:01: 11000000 INFO @ Sat, 15 Jan 2022 17:42:02: 2000000 INFO @ Sat, 15 Jan 2022 17:42:07: 7000000 INFO @ Sat, 15 Jan 2022 17:42:08: 12000000 INFO @ Sat, 15 Jan 2022 17:42:08: 3000000 INFO @ Sat, 15 Jan 2022 17:42:13: 8000000 INFO @ Sat, 15 Jan 2022 17:42:14: 4000000 INFO @ Sat, 15 Jan 2022 17:42:14: 13000000 INFO @ Sat, 15 Jan 2022 17:42:19: 9000000 INFO @ Sat, 15 Jan 2022 17:42:20: 5000000 INFO @ Sat, 15 Jan 2022 17:42:21: 14000000 INFO @ Sat, 15 Jan 2022 17:42:25: 6000000 INFO @ Sat, 15 Jan 2022 17:42:26: 10000000 INFO @ Sat, 15 Jan 2022 17:42:27: 15000000 INFO @ Sat, 15 Jan 2022 17:42:31: 7000000 INFO @ Sat, 15 Jan 2022 17:42:33: 11000000 INFO @ Sat, 15 Jan 2022 17:42:34: 16000000 INFO @ Sat, 15 Jan 2022 17:42:36: 8000000 INFO @ Sat, 15 Jan 2022 17:42:40: 12000000 INFO @ Sat, 15 Jan 2022 17:42:40: 17000000 INFO @ Sat, 15 Jan 2022 17:42:42: 9000000 INFO @ Sat, 15 Jan 2022 17:42:46: 13000000 INFO @ Sat, 15 Jan 2022 17:42:47: 18000000 INFO @ Sat, 15 Jan 2022 17:42:48: 10000000 INFO @ Sat, 15 Jan 2022 17:42:53: 14000000 INFO @ Sat, 15 Jan 2022 17:42:53: 11000000 INFO @ Sat, 15 Jan 2022 17:42:53: 19000000 INFO @ Sat, 15 Jan 2022 17:42:59: 12000000 INFO @ Sat, 15 Jan 2022 17:42:59: 15000000 INFO @ Sat, 15 Jan 2022 17:43:00: 20000000 INFO @ Sat, 15 Jan 2022 17:43:05: 13000000 INFO @ Sat, 15 Jan 2022 17:43:05: 16000000 INFO @ Sat, 15 Jan 2022 17:43:06: 21000000 INFO @ Sat, 15 Jan 2022 17:43:10: 14000000 INFO @ Sat, 15 Jan 2022 17:43:11: 17000000 INFO @ Sat, 15 Jan 2022 17:43:13: 22000000 INFO @ Sat, 15 Jan 2022 17:43:16: 15000000 INFO @ Sat, 15 Jan 2022 17:43:17: 18000000 INFO @ Sat, 15 Jan 2022 17:43:20: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:43:22: 16000000 INFO @ Sat, 15 Jan 2022 17:43:22: 19000000 INFO @ Sat, 15 Jan 2022 17:43:27: 24000000 INFO @ Sat, 15 Jan 2022 17:43:27: 17000000 INFO @ Sat, 15 Jan 2022 17:43:28: 20000000 INFO @ Sat, 15 Jan 2022 17:43:33: 18000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:43:33: 25000000 INFO @ Sat, 15 Jan 2022 17:43:34: 21000000 INFO @ Sat, 15 Jan 2022 17:43:39: 19000000 INFO @ Sat, 15 Jan 2022 17:43:39: 22000000 INFO @ Sat, 15 Jan 2022 17:43:40: 26000000 INFO @ Sat, 15 Jan 2022 17:43:44: 20000000 INFO @ Sat, 15 Jan 2022 17:43:45: 23000000 INFO @ Sat, 15 Jan 2022 17:43:47: 27000000 INFO @ Sat, 15 Jan 2022 17:43:50: 21000000 INFO @ Sat, 15 Jan 2022 17:43:51: 24000000 INFO @ Sat, 15 Jan 2022 17:43:53: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:43:53: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:43:53: #1 total tags in treatment: 12730898 INFO @ Sat, 15 Jan 2022 17:43:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:43:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:43:54: #1 tags after filtering in treatment: 8008738 INFO @ Sat, 15 Jan 2022 17:43:54: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 17:43:54: #1 finished! INFO @ Sat, 15 Jan 2022 17:43:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:43:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:43:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:43:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:43:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:43:56: 22000000 INFO @ Sat, 15 Jan 2022 17:43:56: 25000000 INFO @ Sat, 15 Jan 2022 17:44:01: 23000000 INFO @ Sat, 15 Jan 2022 17:44:02: 26000000 INFO @ Sat, 15 Jan 2022 17:44:07: 24000000 INFO @ Sat, 15 Jan 2022 17:44:08: 27000000 INFO @ Sat, 15 Jan 2022 17:44:12: 25000000 INFO @ Sat, 15 Jan 2022 17:44:14: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:44:14: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:44:14: #1 total tags in treatment: 12730898 INFO @ Sat, 15 Jan 2022 17:44:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:44:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:44:14: #1 tags after filtering in treatment: 8008738 INFO @ Sat, 15 Jan 2022 17:44:14: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 17:44:14: #1 finished! INFO @ Sat, 15 Jan 2022 17:44:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:44:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:44:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:44:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:44:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:44:18: 26000000 INFO @ Sat, 15 Jan 2022 17:44:23: 27000000 INFO @ Sat, 15 Jan 2022 17:44:28: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:44:28: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:44:28: #1 total tags in treatment: 12730898 INFO @ Sat, 15 Jan 2022 17:44:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:44:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:44:28: #1 tags after filtering in treatment: 8008738 INFO @ Sat, 15 Jan 2022 17:44:28: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 17:44:28: #1 finished! INFO @ Sat, 15 Jan 2022 17:44:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:44:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:44:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:44:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:44:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786323/SRX9786323.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling