Job ID = 14519662 SRX = SRX9786322 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10956313 spots for SRR13362154/SRR13362154.sra Written 10956313 spots for SRR13362154/SRR13362154.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:59 10956313 reads; of these: 10956313 (100.00%) were paired; of these: 1691923 (15.44%) aligned concordantly 0 times 8378884 (76.48%) aligned concordantly exactly 1 time 885506 (8.08%) aligned concordantly >1 times ---- 1691923 pairs aligned concordantly 0 times; of these: 156062 (9.22%) aligned discordantly 1 time ---- 1535861 pairs aligned 0 times concordantly or discordantly; of these: 3071722 mates make up the pairs; of these: 1836450 (59.79%) aligned 0 times 1070605 (34.85%) aligned exactly 1 time 164667 (5.36%) aligned >1 times 91.62% overall alignment rate Time searching: 00:08:59 Overall time: 00:08:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 555267 / 9419880 = 0.0589 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:33:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:33:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:33:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:33:46: 1000000 INFO @ Sat, 15 Jan 2022 17:33:53: 2000000 INFO @ Sat, 15 Jan 2022 17:34:00: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:34:07: 4000000 INFO @ Sat, 15 Jan 2022 17:34:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:34:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:34:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:34:15: 5000000 INFO @ Sat, 15 Jan 2022 17:34:17: 1000000 INFO @ Sat, 15 Jan 2022 17:34:22: 6000000 INFO @ Sat, 15 Jan 2022 17:34:24: 2000000 INFO @ Sat, 15 Jan 2022 17:34:30: 7000000 INFO @ Sat, 15 Jan 2022 17:34:32: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:34:37: 8000000 INFO @ Sat, 15 Jan 2022 17:34:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:34:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:34:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:34:40: 4000000 INFO @ Sat, 15 Jan 2022 17:34:45: 9000000 INFO @ Sat, 15 Jan 2022 17:34:47: 1000000 INFO @ Sat, 15 Jan 2022 17:34:48: 5000000 INFO @ Sat, 15 Jan 2022 17:34:53: 10000000 INFO @ Sat, 15 Jan 2022 17:34:54: 2000000 INFO @ Sat, 15 Jan 2022 17:34:56: 6000000 INFO @ Sat, 15 Jan 2022 17:35:01: 11000000 INFO @ Sat, 15 Jan 2022 17:35:02: 3000000 INFO @ Sat, 15 Jan 2022 17:35:03: 7000000 INFO @ Sat, 15 Jan 2022 17:35:09: 12000000 INFO @ Sat, 15 Jan 2022 17:35:10: 4000000 INFO @ Sat, 15 Jan 2022 17:35:11: 8000000 INFO @ Sat, 15 Jan 2022 17:35:16: 13000000 INFO @ Sat, 15 Jan 2022 17:35:18: 5000000 INFO @ Sat, 15 Jan 2022 17:35:19: 9000000 INFO @ Sat, 15 Jan 2022 17:35:24: 14000000 INFO @ Sat, 15 Jan 2022 17:35:26: 6000000 INFO @ Sat, 15 Jan 2022 17:35:27: 10000000 INFO @ Sat, 15 Jan 2022 17:35:32: 15000000 INFO @ Sat, 15 Jan 2022 17:35:33: 7000000 INFO @ Sat, 15 Jan 2022 17:35:35: 11000000 INFO @ Sat, 15 Jan 2022 17:35:39: 16000000 INFO @ Sat, 15 Jan 2022 17:35:41: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:35:43: 12000000 INFO @ Sat, 15 Jan 2022 17:35:47: 17000000 INFO @ Sat, 15 Jan 2022 17:35:49: 9000000 INFO @ Sat, 15 Jan 2022 17:35:51: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:35:55: 18000000 INFO @ Sat, 15 Jan 2022 17:35:57: 10000000 INFO @ Sat, 15 Jan 2022 17:35:59: 14000000 INFO @ Sat, 15 Jan 2022 17:36:03: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:36:03: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:36:03: #1 total tags in treatment: 8712772 INFO @ Sat, 15 Jan 2022 17:36:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:36:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:36:03: #1 tags after filtering in treatment: 5492285 INFO @ Sat, 15 Jan 2022 17:36:03: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 17:36:03: #1 finished! INFO @ Sat, 15 Jan 2022 17:36:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:36:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:36:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:36:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:36:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:36:04: 11000000 INFO @ Sat, 15 Jan 2022 17:36:06: 15000000 INFO @ Sat, 15 Jan 2022 17:36:12: 12000000 INFO @ Sat, 15 Jan 2022 17:36:14: 16000000 INFO @ Sat, 15 Jan 2022 17:36:20: 13000000 INFO @ Sat, 15 Jan 2022 17:36:22: 17000000 INFO @ Sat, 15 Jan 2022 17:36:27: 14000000 INFO @ Sat, 15 Jan 2022 17:36:30: 18000000 INFO @ Sat, 15 Jan 2022 17:36:35: 15000000 INFO @ Sat, 15 Jan 2022 17:36:37: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:36:37: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:36:37: #1 total tags in treatment: 8712772 INFO @ Sat, 15 Jan 2022 17:36:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:36:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:36:37: #1 tags after filtering in treatment: 5492285 INFO @ Sat, 15 Jan 2022 17:36:37: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 17:36:37: #1 finished! INFO @ Sat, 15 Jan 2022 17:36:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:36:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:36:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:36:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:36:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:36:42: 16000000 INFO @ Sat, 15 Jan 2022 17:36:49: 17000000 INFO @ Sat, 15 Jan 2022 17:36:56: 18000000 INFO @ Sat, 15 Jan 2022 17:37:03: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:37:03: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:37:03: #1 total tags in treatment: 8712772 INFO @ Sat, 15 Jan 2022 17:37:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:37:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:37:03: #1 tags after filtering in treatment: 5492285 INFO @ Sat, 15 Jan 2022 17:37:03: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 17:37:03: #1 finished! INFO @ Sat, 15 Jan 2022 17:37:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:37:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:37:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:37:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:37:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786322/SRX9786322.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling