Job ID = 14519661 SRX = SRX9786321 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11772379 spots for SRR13362153/SRR13362153.sra Written 11772379 spots for SRR13362153/SRR13362153.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:18 11772379 reads; of these: 11772379 (100.00%) were paired; of these: 1947334 (16.54%) aligned concordantly 0 times 9015142 (76.58%) aligned concordantly exactly 1 time 809903 (6.88%) aligned concordantly >1 times ---- 1947334 pairs aligned concordantly 0 times; of these: 208925 (10.73%) aligned discordantly 1 time ---- 1738409 pairs aligned 0 times concordantly or discordantly; of these: 3476818 mates make up the pairs; of these: 2078588 (59.78%) aligned 0 times 1224114 (35.21%) aligned exactly 1 time 174116 (5.01%) aligned >1 times 91.17% overall alignment rate Time searching: 00:13:18 Overall time: 00:13:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 655983 / 10033217 = 0.0654 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:39:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:39:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:39:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:39:50: 1000000 INFO @ Sat, 15 Jan 2022 17:40:01: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:40:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:40:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:40:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:40:11: 3000000 INFO @ Sat, 15 Jan 2022 17:40:21: 1000000 INFO @ Sat, 15 Jan 2022 17:40:22: 4000000 INFO @ Sat, 15 Jan 2022 17:40:31: 2000000 INFO @ Sat, 15 Jan 2022 17:40:33: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:40:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:40:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:40:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:40:42: 3000000 INFO @ Sat, 15 Jan 2022 17:40:44: 6000000 INFO @ Sat, 15 Jan 2022 17:40:51: 1000000 INFO @ Sat, 15 Jan 2022 17:40:54: 4000000 INFO @ Sat, 15 Jan 2022 17:40:56: 7000000 INFO @ Sat, 15 Jan 2022 17:41:02: 2000000 INFO @ Sat, 15 Jan 2022 17:41:05: 5000000 INFO @ Sat, 15 Jan 2022 17:41:07: 8000000 INFO @ Sat, 15 Jan 2022 17:41:14: 3000000 INFO @ Sat, 15 Jan 2022 17:41:17: 6000000 INFO @ Sat, 15 Jan 2022 17:41:18: 9000000 INFO @ Sat, 15 Jan 2022 17:41:25: 4000000 INFO @ Sat, 15 Jan 2022 17:41:28: 7000000 INFO @ Sat, 15 Jan 2022 17:41:29: 10000000 INFO @ Sat, 15 Jan 2022 17:41:36: 5000000 INFO @ Sat, 15 Jan 2022 17:41:38: 8000000 INFO @ Sat, 15 Jan 2022 17:41:40: 11000000 INFO @ Sat, 15 Jan 2022 17:41:46: 6000000 INFO @ Sat, 15 Jan 2022 17:41:50: 9000000 INFO @ Sat, 15 Jan 2022 17:41:51: 12000000 INFO @ Sat, 15 Jan 2022 17:41:58: 7000000 INFO @ Sat, 15 Jan 2022 17:42:01: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:42:02: 13000000 INFO @ Sat, 15 Jan 2022 17:42:09: 8000000 INFO @ Sat, 15 Jan 2022 17:42:12: 11000000 INFO @ Sat, 15 Jan 2022 17:42:13: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:42:20: 9000000 INFO @ Sat, 15 Jan 2022 17:42:23: 12000000 INFO @ Sat, 15 Jan 2022 17:42:25: 15000000 INFO @ Sat, 15 Jan 2022 17:42:31: 10000000 INFO @ Sat, 15 Jan 2022 17:42:34: 13000000 INFO @ Sat, 15 Jan 2022 17:42:36: 16000000 INFO @ Sat, 15 Jan 2022 17:42:42: 11000000 INFO @ Sat, 15 Jan 2022 17:42:46: 14000000 INFO @ Sat, 15 Jan 2022 17:42:47: 17000000 INFO @ Sat, 15 Jan 2022 17:42:54: 12000000 INFO @ Sat, 15 Jan 2022 17:42:57: 15000000 INFO @ Sat, 15 Jan 2022 17:42:59: 18000000 INFO @ Sat, 15 Jan 2022 17:43:05: 13000000 INFO @ Sat, 15 Jan 2022 17:43:08: 16000000 INFO @ Sat, 15 Jan 2022 17:43:10: 19000000 INFO @ Sat, 15 Jan 2022 17:43:17: 14000000 INFO @ Sat, 15 Jan 2022 17:43:20: 17000000 INFO @ Sat, 15 Jan 2022 17:43:22: 20000000 INFO @ Sat, 15 Jan 2022 17:43:23: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:43:23: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:43:23: #1 total tags in treatment: 9174299 INFO @ Sat, 15 Jan 2022 17:43:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:43:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:43:23: #1 tags after filtering in treatment: 5423241 INFO @ Sat, 15 Jan 2022 17:43:23: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 17:43:23: #1 finished! INFO @ Sat, 15 Jan 2022 17:43:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:43:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:43:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:43:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:43:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:43:28: 15000000 INFO @ Sat, 15 Jan 2022 17:43:31: 18000000 INFO @ Sat, 15 Jan 2022 17:43:39: 16000000 INFO @ Sat, 15 Jan 2022 17:43:42: 19000000 INFO @ Sat, 15 Jan 2022 17:43:49: 17000000 INFO @ Sat, 15 Jan 2022 17:43:53: 20000000 INFO @ Sat, 15 Jan 2022 17:43:54: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:43:54: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:43:54: #1 total tags in treatment: 9174299 INFO @ Sat, 15 Jan 2022 17:43:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:43:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:43:54: #1 tags after filtering in treatment: 5423241 INFO @ Sat, 15 Jan 2022 17:43:54: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 17:43:54: #1 finished! INFO @ Sat, 15 Jan 2022 17:43:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:43:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:43:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:43:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:43:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:44:00: 18000000 INFO @ Sat, 15 Jan 2022 17:44:11: 19000000 INFO @ Sat, 15 Jan 2022 17:44:22: 20000000 INFO @ Sat, 15 Jan 2022 17:44:23: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 17:44:23: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 17:44:23: #1 total tags in treatment: 9174299 INFO @ Sat, 15 Jan 2022 17:44:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:44:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:44:23: #1 tags after filtering in treatment: 5423241 INFO @ Sat, 15 Jan 2022 17:44:23: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 17:44:23: #1 finished! INFO @ Sat, 15 Jan 2022 17:44:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:44:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:44:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:44:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:44:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786321/SRX9786321.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling