Job ID = 14522164 SRX = SRX9786319 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11244513 spots for SRR13362183/SRR13362183.sra Written 11244513 spots for SRR13362183/SRR13362183.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:11 11244513 reads; of these: 11244513 (100.00%) were paired; of these: 5376620 (47.82%) aligned concordantly 0 times 5305093 (47.18%) aligned concordantly exactly 1 time 562800 (5.01%) aligned concordantly >1 times ---- 5376620 pairs aligned concordantly 0 times; of these: 120048 (2.23%) aligned discordantly 1 time ---- 5256572 pairs aligned 0 times concordantly or discordantly; of these: 10513144 mates make up the pairs; of these: 5673307 (53.96%) aligned 0 times 4310063 (41.00%) aligned exactly 1 time 529774 (5.04%) aligned >1 times 74.77% overall alignment rate Time searching: 00:06:11 Overall time: 00:06:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 37624 / 5987257 = 0.0063 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:27:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:27:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:27:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:27:20: 1000000 INFO @ Sat, 15 Jan 2022 22:27:25: 2000000 INFO @ Sat, 15 Jan 2022 22:27:29: 3000000 INFO @ Sat, 15 Jan 2022 22:27:34: 4000000 INFO @ Sat, 15 Jan 2022 22:27:38: 5000000 INFO @ Sat, 15 Jan 2022 22:27:43: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:27:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:27:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:27:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:27:48: 7000000 INFO @ Sat, 15 Jan 2022 22:27:51: 1000000 INFO @ Sat, 15 Jan 2022 22:27:53: 8000000 INFO @ Sat, 15 Jan 2022 22:27:57: 2000000 INFO @ Sat, 15 Jan 2022 22:27:58: 9000000 INFO @ Sat, 15 Jan 2022 22:28:02: 3000000 INFO @ Sat, 15 Jan 2022 22:28:03: 10000000 INFO @ Sat, 15 Jan 2022 22:28:08: 11000000 INFO @ Sat, 15 Jan 2022 22:28:08: 4000000 INFO @ Sat, 15 Jan 2022 22:28:13: 12000000 INFO @ Sat, 15 Jan 2022 22:28:14: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:28:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:28:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:28:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:28:18: 13000000 INFO @ Sat, 15 Jan 2022 22:28:19: 6000000 INFO @ Sat, 15 Jan 2022 22:28:21: 1000000 INFO @ Sat, 15 Jan 2022 22:28:23: 14000000 INFO @ Sat, 15 Jan 2022 22:28:25: 7000000 INFO @ Sat, 15 Jan 2022 22:28:27: 2000000 INFO @ Sat, 15 Jan 2022 22:28:28: 15000000 INFO @ Sat, 15 Jan 2022 22:28:31: 8000000 INFO @ Sat, 15 Jan 2022 22:28:32: 3000000 INFO @ Sat, 15 Jan 2022 22:28:33: 16000000 INFO @ Sat, 15 Jan 2022 22:28:36: 9000000 INFO @ Sat, 15 Jan 2022 22:28:37: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:28:37: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:28:37: #1 total tags in treatment: 5830403 INFO @ Sat, 15 Jan 2022 22:28:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:28:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:28:37: #1 tags after filtering in treatment: 4916637 INFO @ Sat, 15 Jan 2022 22:28:37: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 15 Jan 2022 22:28:37: #1 finished! INFO @ Sat, 15 Jan 2022 22:28:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:28:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:28:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:28:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:28:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:28:38: 4000000 INFO @ Sat, 15 Jan 2022 22:28:42: 10000000 INFO @ Sat, 15 Jan 2022 22:28:44: 5000000 INFO @ Sat, 15 Jan 2022 22:28:48: 11000000 INFO @ Sat, 15 Jan 2022 22:28:50: 6000000 INFO @ Sat, 15 Jan 2022 22:28:54: 12000000 INFO @ Sat, 15 Jan 2022 22:28:55: 7000000 INFO @ Sat, 15 Jan 2022 22:29:00: 13000000 INFO @ Sat, 15 Jan 2022 22:29:01: 8000000 INFO @ Sat, 15 Jan 2022 22:29:06: 14000000 INFO @ Sat, 15 Jan 2022 22:29:07: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:29:12: 15000000 INFO @ Sat, 15 Jan 2022 22:29:13: 10000000 INFO @ Sat, 15 Jan 2022 22:29:18: 16000000 INFO @ Sat, 15 Jan 2022 22:29:19: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:29:22: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:29:22: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:29:22: #1 total tags in treatment: 5830403 INFO @ Sat, 15 Jan 2022 22:29:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:29:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:29:22: #1 tags after filtering in treatment: 4916637 INFO @ Sat, 15 Jan 2022 22:29:22: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 15 Jan 2022 22:29:22: #1 finished! INFO @ Sat, 15 Jan 2022 22:29:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:29:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:29:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:29:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:29:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:29:24: 12000000 INFO @ Sat, 15 Jan 2022 22:29:30: 13000000 INFO @ Sat, 15 Jan 2022 22:29:36: 14000000 INFO @ Sat, 15 Jan 2022 22:29:42: 15000000 INFO @ Sat, 15 Jan 2022 22:29:47: 16000000 INFO @ Sat, 15 Jan 2022 22:29:51: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:29:51: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:29:51: #1 total tags in treatment: 5830403 INFO @ Sat, 15 Jan 2022 22:29:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:29:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:29:51: #1 tags after filtering in treatment: 4916637 INFO @ Sat, 15 Jan 2022 22:29:51: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 15 Jan 2022 22:29:51: #1 finished! INFO @ Sat, 15 Jan 2022 22:29:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:29:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:29:52: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:29:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:29:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786319/SRX9786319.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling