Job ID = 14522163 SRX = SRX9786318 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16989726 spots for SRR13362182/SRR13362182.sra Written 16989726 spots for SRR13362182/SRR13362182.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:32 16989726 reads; of these: 16989726 (100.00%) were paired; of these: 6989202 (41.14%) aligned concordantly 0 times 9147600 (53.84%) aligned concordantly exactly 1 time 852924 (5.02%) aligned concordantly >1 times ---- 6989202 pairs aligned concordantly 0 times; of these: 199271 (2.85%) aligned discordantly 1 time ---- 6789931 pairs aligned 0 times concordantly or discordantly; of these: 13579862 mates make up the pairs; of these: 7491926 (55.17%) aligned 0 times 5468774 (40.27%) aligned exactly 1 time 619162 (4.56%) aligned >1 times 77.95% overall alignment rate Time searching: 00:09:32 Overall time: 00:09:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 71720 / 10198607 = 0.0070 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:34:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:34:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:34:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:34:27: 1000000 INFO @ Sat, 15 Jan 2022 22:34:32: 2000000 INFO @ Sat, 15 Jan 2022 22:34:37: 3000000 INFO @ Sat, 15 Jan 2022 22:34:42: 4000000 INFO @ Sat, 15 Jan 2022 22:34:48: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:34:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:34:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:34:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:34:53: 6000000 INFO @ Sat, 15 Jan 2022 22:34:58: 1000000 INFO @ Sat, 15 Jan 2022 22:34:58: 7000000 INFO @ Sat, 15 Jan 2022 22:35:03: 2000000 INFO @ Sat, 15 Jan 2022 22:35:04: 8000000 INFO @ Sat, 15 Jan 2022 22:35:09: 3000000 INFO @ Sat, 15 Jan 2022 22:35:10: 9000000 INFO @ Sat, 15 Jan 2022 22:35:15: 4000000 INFO @ Sat, 15 Jan 2022 22:35:16: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:35:21: 5000000 INFO @ Sat, 15 Jan 2022 22:35:21: 11000000 INFO @ Sat, 15 Jan 2022 22:35:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:35:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:35:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:35:27: 6000000 INFO @ Sat, 15 Jan 2022 22:35:27: 12000000 INFO @ Sat, 15 Jan 2022 22:35:28: 1000000 INFO @ Sat, 15 Jan 2022 22:35:32: 7000000 INFO @ Sat, 15 Jan 2022 22:35:33: 13000000 INFO @ Sat, 15 Jan 2022 22:35:34: 2000000 INFO @ Sat, 15 Jan 2022 22:35:38: 8000000 INFO @ Sat, 15 Jan 2022 22:35:39: 14000000 INFO @ Sat, 15 Jan 2022 22:35:39: 3000000 INFO @ Sat, 15 Jan 2022 22:35:44: 9000000 INFO @ Sat, 15 Jan 2022 22:35:45: 15000000 INFO @ Sat, 15 Jan 2022 22:35:45: 4000000 INFO @ Sat, 15 Jan 2022 22:35:50: 10000000 INFO @ Sat, 15 Jan 2022 22:35:51: 16000000 INFO @ Sat, 15 Jan 2022 22:35:51: 5000000 INFO @ Sat, 15 Jan 2022 22:35:56: 11000000 INFO @ Sat, 15 Jan 2022 22:35:57: 17000000 INFO @ Sat, 15 Jan 2022 22:35:57: 6000000 INFO @ Sat, 15 Jan 2022 22:36:02: 12000000 INFO @ Sat, 15 Jan 2022 22:36:02: 18000000 INFO @ Sat, 15 Jan 2022 22:36:03: 7000000 INFO @ Sat, 15 Jan 2022 22:36:07: 13000000 INFO @ Sat, 15 Jan 2022 22:36:08: 19000000 INFO @ Sat, 15 Jan 2022 22:36:08: 8000000 INFO @ Sat, 15 Jan 2022 22:36:13: 14000000 INFO @ Sat, 15 Jan 2022 22:36:14: 20000000 INFO @ Sat, 15 Jan 2022 22:36:14: 9000000 INFO @ Sat, 15 Jan 2022 22:36:19: 15000000 INFO @ Sat, 15 Jan 2022 22:36:20: 21000000 INFO @ Sat, 15 Jan 2022 22:36:20: 10000000 INFO @ Sat, 15 Jan 2022 22:36:25: 16000000 INFO @ Sat, 15 Jan 2022 22:36:26: 22000000 INFO @ Sat, 15 Jan 2022 22:36:26: 11000000 INFO @ Sat, 15 Jan 2022 22:36:31: 17000000 INFO @ Sat, 15 Jan 2022 22:36:32: 12000000 INFO @ Sat, 15 Jan 2022 22:36:32: 23000000 INFO @ Sat, 15 Jan 2022 22:36:37: 18000000 INFO @ Sat, 15 Jan 2022 22:36:38: 13000000 INFO @ Sat, 15 Jan 2022 22:36:38: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:36:42: 19000000 INFO @ Sat, 15 Jan 2022 22:36:43: 14000000 INFO @ Sat, 15 Jan 2022 22:36:44: 25000000 INFO @ Sat, 15 Jan 2022 22:36:48: 20000000 INFO @ Sat, 15 Jan 2022 22:36:49: 15000000 INFO @ Sat, 15 Jan 2022 22:36:49: 26000000 INFO @ Sat, 15 Jan 2022 22:36:51: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:36:51: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:36:51: #1 total tags in treatment: 9929014 INFO @ Sat, 15 Jan 2022 22:36:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:36:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:36:52: #1 tags after filtering in treatment: 7640442 INFO @ Sat, 15 Jan 2022 22:36:52: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 22:36:52: #1 finished! INFO @ Sat, 15 Jan 2022 22:36:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:36:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:36:52: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:36:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:36:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:36:54: 21000000 INFO @ Sat, 15 Jan 2022 22:36:55: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:37:00: 22000000 INFO @ Sat, 15 Jan 2022 22:37:01: 17000000 INFO @ Sat, 15 Jan 2022 22:37:05: 23000000 INFO @ Sat, 15 Jan 2022 22:37:06: 18000000 INFO @ Sat, 15 Jan 2022 22:37:11: 24000000 INFO @ Sat, 15 Jan 2022 22:37:12: 19000000 INFO @ Sat, 15 Jan 2022 22:37:17: 25000000 INFO @ Sat, 15 Jan 2022 22:37:18: 20000000 INFO @ Sat, 15 Jan 2022 22:37:22: 26000000 INFO @ Sat, 15 Jan 2022 22:37:23: 21000000 INFO @ Sat, 15 Jan 2022 22:37:24: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:37:24: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:37:24: #1 total tags in treatment: 9929014 INFO @ Sat, 15 Jan 2022 22:37:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:37:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:37:25: #1 tags after filtering in treatment: 7640442 INFO @ Sat, 15 Jan 2022 22:37:25: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 22:37:25: #1 finished! INFO @ Sat, 15 Jan 2022 22:37:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:37:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:37:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:37:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:37:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:37:28: 22000000 INFO @ Sat, 15 Jan 2022 22:37:34: 23000000 INFO @ Sat, 15 Jan 2022 22:37:39: 24000000 INFO @ Sat, 15 Jan 2022 22:37:43: 25000000 INFO @ Sat, 15 Jan 2022 22:37:48: 26000000 INFO @ Sat, 15 Jan 2022 22:37:50: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:37:50: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:37:50: #1 total tags in treatment: 9929014 INFO @ Sat, 15 Jan 2022 22:37:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:37:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:37:50: #1 tags after filtering in treatment: 7640442 INFO @ Sat, 15 Jan 2022 22:37:50: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 22:37:50: #1 finished! INFO @ Sat, 15 Jan 2022 22:37:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:37:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:37:51: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:37:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:37:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786318/SRX9786318.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling