Job ID = 14522162 SRX = SRX9786317 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10317697 spots for SRR13362181/SRR13362181.sra Written 10317697 spots for SRR13362181/SRR13362181.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:14 10317697 reads; of these: 10317697 (100.00%) were paired; of these: 4014129 (38.91%) aligned concordantly 0 times 5788045 (56.10%) aligned concordantly exactly 1 time 515523 (5.00%) aligned concordantly >1 times ---- 4014129 pairs aligned concordantly 0 times; of these: 118797 (2.96%) aligned discordantly 1 time ---- 3895332 pairs aligned 0 times concordantly or discordantly; of these: 7790664 mates make up the pairs; of these: 4376708 (56.18%) aligned 0 times 3075593 (39.48%) aligned exactly 1 time 338363 (4.34%) aligned >1 times 78.79% overall alignment rate Time searching: 00:06:14 Overall time: 00:06:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 50099 / 6421685 = 0.0078 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:28:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:28:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:28:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:28:08: 1000000 INFO @ Sat, 15 Jan 2022 22:28:13: 2000000 INFO @ Sat, 15 Jan 2022 22:28:18: 3000000 INFO @ Sat, 15 Jan 2022 22:28:23: 4000000 INFO @ Sat, 15 Jan 2022 22:28:29: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:28:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:28:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:28:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:28:34: 6000000 INFO @ Sat, 15 Jan 2022 22:28:38: 1000000 INFO @ Sat, 15 Jan 2022 22:28:39: 7000000 INFO @ Sat, 15 Jan 2022 22:28:43: 2000000 INFO @ Sat, 15 Jan 2022 22:28:44: 8000000 INFO @ Sat, 15 Jan 2022 22:28:49: 3000000 INFO @ Sat, 15 Jan 2022 22:28:49: 9000000 INFO @ Sat, 15 Jan 2022 22:28:54: 4000000 INFO @ Sat, 15 Jan 2022 22:28:55: 10000000 INFO @ Sat, 15 Jan 2022 22:28:59: 5000000 INFO @ Sat, 15 Jan 2022 22:29:00: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:29:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:29:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:29:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:29:04: 6000000 INFO @ Sat, 15 Jan 2022 22:29:05: 12000000 INFO @ Sat, 15 Jan 2022 22:29:08: 1000000 INFO @ Sat, 15 Jan 2022 22:29:09: 7000000 INFO @ Sat, 15 Jan 2022 22:29:10: 13000000 INFO @ Sat, 15 Jan 2022 22:29:14: 2000000 INFO @ Sat, 15 Jan 2022 22:29:15: 8000000 INFO @ Sat, 15 Jan 2022 22:29:15: 14000000 INFO @ Sat, 15 Jan 2022 22:29:19: 3000000 INFO @ Sat, 15 Jan 2022 22:29:20: 9000000 INFO @ Sat, 15 Jan 2022 22:29:20: 15000000 INFO @ Sat, 15 Jan 2022 22:29:24: 4000000 INFO @ Sat, 15 Jan 2022 22:29:25: 10000000 INFO @ Sat, 15 Jan 2022 22:29:26: 16000000 INFO @ Sat, 15 Jan 2022 22:29:27: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:29:27: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:29:27: #1 total tags in treatment: 6253638 INFO @ Sat, 15 Jan 2022 22:29:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:29:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:29:27: #1 tags after filtering in treatment: 5233987 INFO @ Sat, 15 Jan 2022 22:29:27: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 15 Jan 2022 22:29:27: #1 finished! INFO @ Sat, 15 Jan 2022 22:29:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:29:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:29:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:29:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:29:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:29:29: 5000000 INFO @ Sat, 15 Jan 2022 22:29:30: 11000000 INFO @ Sat, 15 Jan 2022 22:29:35: 6000000 INFO @ Sat, 15 Jan 2022 22:29:35: 12000000 INFO @ Sat, 15 Jan 2022 22:29:40: 7000000 INFO @ Sat, 15 Jan 2022 22:29:41: 13000000 INFO @ Sat, 15 Jan 2022 22:29:45: 8000000 INFO @ Sat, 15 Jan 2022 22:29:46: 14000000 INFO @ Sat, 15 Jan 2022 22:29:50: 9000000 INFO @ Sat, 15 Jan 2022 22:29:51: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:29:55: 10000000 INFO @ Sat, 15 Jan 2022 22:29:56: 16000000 INFO @ Sat, 15 Jan 2022 22:29:57: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:29:57: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:29:57: #1 total tags in treatment: 6253638 INFO @ Sat, 15 Jan 2022 22:29:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:29:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:29:57: #1 tags after filtering in treatment: 5233987 INFO @ Sat, 15 Jan 2022 22:29:57: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 15 Jan 2022 22:29:57: #1 finished! INFO @ Sat, 15 Jan 2022 22:29:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:29:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:29:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:29:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:29:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:30:01: 11000000 INFO @ Sat, 15 Jan 2022 22:30:05: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:30:11: 13000000 INFO @ Sat, 15 Jan 2022 22:30:16: 14000000 INFO @ Sat, 15 Jan 2022 22:30:21: 15000000 INFO @ Sat, 15 Jan 2022 22:30:26: 16000000 INFO @ Sat, 15 Jan 2022 22:30:27: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:30:27: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:30:27: #1 total tags in treatment: 6253638 INFO @ Sat, 15 Jan 2022 22:30:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:30:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:30:27: #1 tags after filtering in treatment: 5233987 INFO @ Sat, 15 Jan 2022 22:30:27: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 15 Jan 2022 22:30:27: #1 finished! INFO @ Sat, 15 Jan 2022 22:30:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:30:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:30:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:30:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:30:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786317/SRX9786317.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling