Job ID = 14522161 SRX = SRX9786316 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11338094 spots for SRR13362180/SRR13362180.sra Written 11338094 spots for SRR13362180/SRR13362180.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:22 11338094 reads; of these: 11338094 (100.00%) were paired; of these: 4627394 (40.81%) aligned concordantly 0 times 6212811 (54.80%) aligned concordantly exactly 1 time 497889 (4.39%) aligned concordantly >1 times ---- 4627394 pairs aligned concordantly 0 times; of these: 184343 (3.98%) aligned discordantly 1 time ---- 4443051 pairs aligned 0 times concordantly or discordantly; of these: 8886102 mates make up the pairs; of these: 4991176 (56.17%) aligned 0 times 3527448 (39.70%) aligned exactly 1 time 367478 (4.14%) aligned >1 times 77.99% overall alignment rate Time searching: 00:10:22 Overall time: 00:10:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 89723 / 6894225 = 0.0130 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:34:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:34:48: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:34:48: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:34:57: 1000000 INFO @ Sat, 15 Jan 2022 22:35:07: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:35:17: 3000000 INFO @ Sat, 15 Jan 2022 22:35:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:35:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:35:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:35:27: 1000000 INFO @ Sat, 15 Jan 2022 22:35:28: 4000000 INFO @ Sat, 15 Jan 2022 22:35:37: 2000000 INFO @ Sat, 15 Jan 2022 22:35:39: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:35:46: 3000000 INFO @ Sat, 15 Jan 2022 22:35:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:35:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:35:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:35:50: 6000000 INFO @ Sat, 15 Jan 2022 22:35:56: 4000000 INFO @ Sat, 15 Jan 2022 22:35:57: 1000000 INFO @ Sat, 15 Jan 2022 22:36:00: 7000000 INFO @ Sat, 15 Jan 2022 22:36:06: 5000000 INFO @ Sat, 15 Jan 2022 22:36:07: 2000000 INFO @ Sat, 15 Jan 2022 22:36:11: 8000000 INFO @ Sat, 15 Jan 2022 22:36:16: 6000000 INFO @ Sat, 15 Jan 2022 22:36:17: 3000000 INFO @ Sat, 15 Jan 2022 22:36:22: 9000000 INFO @ Sat, 15 Jan 2022 22:36:25: 7000000 INFO @ Sat, 15 Jan 2022 22:36:27: 4000000 INFO @ Sat, 15 Jan 2022 22:36:32: 10000000 INFO @ Sat, 15 Jan 2022 22:36:35: 8000000 INFO @ Sat, 15 Jan 2022 22:36:37: 5000000 INFO @ Sat, 15 Jan 2022 22:36:43: 11000000 INFO @ Sat, 15 Jan 2022 22:36:45: 9000000 INFO @ Sat, 15 Jan 2022 22:36:46: 6000000 INFO @ Sat, 15 Jan 2022 22:36:53: 12000000 INFO @ Sat, 15 Jan 2022 22:36:54: 10000000 INFO @ Sat, 15 Jan 2022 22:36:56: 7000000 INFO @ Sat, 15 Jan 2022 22:37:04: 11000000 INFO @ Sat, 15 Jan 2022 22:37:04: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:37:06: 8000000 INFO @ Sat, 15 Jan 2022 22:37:13: 12000000 INFO @ Sat, 15 Jan 2022 22:37:15: 14000000 INFO @ Sat, 15 Jan 2022 22:37:15: 9000000 INFO @ Sat, 15 Jan 2022 22:37:23: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:37:25: 10000000 INFO @ Sat, 15 Jan 2022 22:37:25: 15000000 INFO @ Sat, 15 Jan 2022 22:37:32: 14000000 INFO @ Sat, 15 Jan 2022 22:37:35: 11000000 INFO @ Sat, 15 Jan 2022 22:37:36: 16000000 INFO @ Sat, 15 Jan 2022 22:37:42: 15000000 INFO @ Sat, 15 Jan 2022 22:37:44: 12000000 INFO @ Sat, 15 Jan 2022 22:37:46: 17000000 INFO @ Sat, 15 Jan 2022 22:37:51: 16000000 INFO @ Sat, 15 Jan 2022 22:37:52: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:37:52: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:37:52: #1 total tags in treatment: 6621563 INFO @ Sat, 15 Jan 2022 22:37:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:37:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:37:52: #1 tags after filtering in treatment: 5488815 INFO @ Sat, 15 Jan 2022 22:37:52: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 22:37:52: #1 finished! INFO @ Sat, 15 Jan 2022 22:37:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:37:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:37:52: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:37:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:37:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:37:54: 13000000 INFO @ Sat, 15 Jan 2022 22:38:00: 17000000 INFO @ Sat, 15 Jan 2022 22:38:04: 14000000 INFO @ Sat, 15 Jan 2022 22:38:05: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:38:05: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:38:05: #1 total tags in treatment: 6621563 INFO @ Sat, 15 Jan 2022 22:38:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:38:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:38:05: #1 tags after filtering in treatment: 5488815 INFO @ Sat, 15 Jan 2022 22:38:05: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 22:38:05: #1 finished! INFO @ Sat, 15 Jan 2022 22:38:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:38:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:38:05: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:38:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:38:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:38:13: 15000000 INFO @ Sat, 15 Jan 2022 22:38:21: 16000000 INFO @ Sat, 15 Jan 2022 22:38:30: 17000000 INFO @ Sat, 15 Jan 2022 22:38:34: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:38:34: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:38:34: #1 total tags in treatment: 6621563 INFO @ Sat, 15 Jan 2022 22:38:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:38:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:38:34: #1 tags after filtering in treatment: 5488815 INFO @ Sat, 15 Jan 2022 22:38:34: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 15 Jan 2022 22:38:34: #1 finished! INFO @ Sat, 15 Jan 2022 22:38:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:38:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:38:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:38:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:38:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786316/SRX9786316.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling