Job ID = 14522160 SRX = SRX9786315 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 14875522 spots for SRR13362179/SRR13362179.sra Written 14875522 spots for SRR13362179/SRR13362179.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:55 14875522 reads; of these: 14875522 (100.00%) were paired; of these: 5903980 (39.69%) aligned concordantly 0 times 8163384 (54.88%) aligned concordantly exactly 1 time 808158 (5.43%) aligned concordantly >1 times ---- 5903980 pairs aligned concordantly 0 times; of these: 213543 (3.62%) aligned discordantly 1 time ---- 5690437 pairs aligned 0 times concordantly or discordantly; of these: 11380874 mates make up the pairs; of these: 6292756 (55.29%) aligned 0 times 4535911 (39.86%) aligned exactly 1 time 552207 (4.85%) aligned >1 times 78.85% overall alignment rate Time searching: 00:12:55 Overall time: 00:12:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 81282 / 9184112 = 0.0089 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:38:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:38:14: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:38:14: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:38:20: 1000000 INFO @ Sat, 15 Jan 2022 22:38:25: 2000000 INFO @ Sat, 15 Jan 2022 22:38:31: 3000000 INFO @ Sat, 15 Jan 2022 22:38:36: 4000000 INFO @ Sat, 15 Jan 2022 22:38:42: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:38:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:38:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:38:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:38:47: 6000000 INFO @ Sat, 15 Jan 2022 22:38:51: 1000000 INFO @ Sat, 15 Jan 2022 22:38:53: 7000000 INFO @ Sat, 15 Jan 2022 22:38:58: 2000000 INFO @ Sat, 15 Jan 2022 22:38:59: 8000000 INFO @ Sat, 15 Jan 2022 22:39:04: 3000000 INFO @ Sat, 15 Jan 2022 22:39:05: 9000000 INFO @ Sat, 15 Jan 2022 22:39:10: 10000000 INFO @ Sat, 15 Jan 2022 22:39:11: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:39:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:39:14: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:39:14: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:39:16: 11000000 INFO @ Sat, 15 Jan 2022 22:39:17: 5000000 INFO @ Sat, 15 Jan 2022 22:39:21: 1000000 INFO @ Sat, 15 Jan 2022 22:39:23: 12000000 INFO @ Sat, 15 Jan 2022 22:39:24: 6000000 INFO @ Sat, 15 Jan 2022 22:39:26: 2000000 INFO @ Sat, 15 Jan 2022 22:39:29: 13000000 INFO @ Sat, 15 Jan 2022 22:39:31: 7000000 INFO @ Sat, 15 Jan 2022 22:39:32: 3000000 INFO @ Sat, 15 Jan 2022 22:39:35: 14000000 INFO @ Sat, 15 Jan 2022 22:39:38: 8000000 INFO @ Sat, 15 Jan 2022 22:39:38: 4000000 INFO @ Sat, 15 Jan 2022 22:39:41: 15000000 INFO @ Sat, 15 Jan 2022 22:39:44: 5000000 INFO @ Sat, 15 Jan 2022 22:39:45: 9000000 INFO @ Sat, 15 Jan 2022 22:39:47: 16000000 INFO @ Sat, 15 Jan 2022 22:39:51: 6000000 INFO @ Sat, 15 Jan 2022 22:39:51: 10000000 INFO @ Sat, 15 Jan 2022 22:39:53: 17000000 INFO @ Sat, 15 Jan 2022 22:39:57: 7000000 INFO @ Sat, 15 Jan 2022 22:39:58: 11000000 INFO @ Sat, 15 Jan 2022 22:40:00: 18000000 INFO @ Sat, 15 Jan 2022 22:40:03: 8000000 INFO @ Sat, 15 Jan 2022 22:40:05: 12000000 INFO @ Sat, 15 Jan 2022 22:40:06: 19000000 INFO @ Sat, 15 Jan 2022 22:40:09: 9000000 INFO @ Sat, 15 Jan 2022 22:40:12: 13000000 INFO @ Sat, 15 Jan 2022 22:40:12: 20000000 INFO @ Sat, 15 Jan 2022 22:40:15: 10000000 INFO @ Sat, 15 Jan 2022 22:40:18: 21000000 INFO @ Sat, 15 Jan 2022 22:40:19: 14000000 INFO @ Sat, 15 Jan 2022 22:40:21: 11000000 INFO @ Sat, 15 Jan 2022 22:40:24: 22000000 INFO @ Sat, 15 Jan 2022 22:40:25: 15000000 INFO @ Sat, 15 Jan 2022 22:40:26: 12000000 INFO @ Sat, 15 Jan 2022 22:40:30: 23000000 INFO @ Sat, 15 Jan 2022 22:40:32: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:40:32: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:40:32: #1 total tags in treatment: 8890637 INFO @ Sat, 15 Jan 2022 22:40:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:40:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:40:32: #1 tags after filtering in treatment: 7006461 INFO @ Sat, 15 Jan 2022 22:40:32: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 22:40:32: #1 finished! INFO @ Sat, 15 Jan 2022 22:40:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:40:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:40:32: 16000000 INFO @ Sat, 15 Jan 2022 22:40:32: 13000000 INFO @ Sat, 15 Jan 2022 22:40:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:40:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:40:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:40:38: 14000000 INFO @ Sat, 15 Jan 2022 22:40:39: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:40:43: 15000000 INFO @ Sat, 15 Jan 2022 22:40:45: 18000000 INFO @ Sat, 15 Jan 2022 22:40:49: 16000000 INFO @ Sat, 15 Jan 2022 22:40:52: 19000000 INFO @ Sat, 15 Jan 2022 22:40:55: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:40:58: 20000000 INFO @ Sat, 15 Jan 2022 22:41:00: 18000000 INFO @ Sat, 15 Jan 2022 22:41:05: 21000000 INFO @ Sat, 15 Jan 2022 22:41:06: 19000000 INFO @ Sat, 15 Jan 2022 22:41:11: 22000000 INFO @ Sat, 15 Jan 2022 22:41:11: 20000000 INFO @ Sat, 15 Jan 2022 22:41:17: 21000000 INFO @ Sat, 15 Jan 2022 22:41:18: 23000000 INFO @ Sat, 15 Jan 2022 22:41:20: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:41:20: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:41:20: #1 total tags in treatment: 8890637 INFO @ Sat, 15 Jan 2022 22:41:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:41:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:41:20: #1 tags after filtering in treatment: 7006461 INFO @ Sat, 15 Jan 2022 22:41:20: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 22:41:20: #1 finished! INFO @ Sat, 15 Jan 2022 22:41:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:41:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:41:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:41:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:41:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:41:22: 22000000 INFO @ Sat, 15 Jan 2022 22:41:28: 23000000 INFO @ Sat, 15 Jan 2022 22:41:29: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:41:29: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:41:29: #1 total tags in treatment: 8890637 INFO @ Sat, 15 Jan 2022 22:41:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:41:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:41:29: #1 tags after filtering in treatment: 7006461 INFO @ Sat, 15 Jan 2022 22:41:29: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 22:41:29: #1 finished! INFO @ Sat, 15 Jan 2022 22:41:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:41:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:41:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:41:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:41:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786315/SRX9786315.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling