Job ID = 14522158 SRX = SRX9786313 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 13874551 spots for SRR13362177/SRR13362177.sra Written 13874551 spots for SRR13362177/SRR13362177.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:14 13874551 reads; of these: 13874551 (100.00%) were paired; of these: 5881038 (42.39%) aligned concordantly 0 times 7366920 (53.10%) aligned concordantly exactly 1 time 626593 (4.52%) aligned concordantly >1 times ---- 5881038 pairs aligned concordantly 0 times; of these: 253057 (4.30%) aligned discordantly 1 time ---- 5627981 pairs aligned 0 times concordantly or discordantly; of these: 11255962 mates make up the pairs; of these: 6299964 (55.97%) aligned 0 times 4463741 (39.66%) aligned exactly 1 time 492257 (4.37%) aligned >1 times 77.30% overall alignment rate Time searching: 00:12:14 Overall time: 00:12:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 91758 / 8245604 = 0.0111 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:36:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:36:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:36:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:36:56: 1000000 INFO @ Sat, 15 Jan 2022 22:37:02: 2000000 INFO @ Sat, 15 Jan 2022 22:37:07: 3000000 INFO @ Sat, 15 Jan 2022 22:37:12: 4000000 INFO @ Sat, 15 Jan 2022 22:37:17: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:37:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:37:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:37:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:37:22: 6000000 INFO @ Sat, 15 Jan 2022 22:37:27: 1000000 INFO @ Sat, 15 Jan 2022 22:37:28: 7000000 INFO @ Sat, 15 Jan 2022 22:37:32: 2000000 INFO @ Sat, 15 Jan 2022 22:37:33: 8000000 INFO @ Sat, 15 Jan 2022 22:37:38: 3000000 INFO @ Sat, 15 Jan 2022 22:37:39: 9000000 INFO @ Sat, 15 Jan 2022 22:37:44: 4000000 INFO @ Sat, 15 Jan 2022 22:37:45: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:37:50: 5000000 INFO @ Sat, 15 Jan 2022 22:37:50: 11000000 INFO @ Sat, 15 Jan 2022 22:37:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:37:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:37:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:37:56: 6000000 INFO @ Sat, 15 Jan 2022 22:37:57: 12000000 INFO @ Sat, 15 Jan 2022 22:37:58: 1000000 INFO @ Sat, 15 Jan 2022 22:38:02: 7000000 INFO @ Sat, 15 Jan 2022 22:38:03: 13000000 INFO @ Sat, 15 Jan 2022 22:38:04: 2000000 INFO @ Sat, 15 Jan 2022 22:38:09: 8000000 INFO @ Sat, 15 Jan 2022 22:38:10: 14000000 INFO @ Sat, 15 Jan 2022 22:38:11: 3000000 INFO @ Sat, 15 Jan 2022 22:38:15: 9000000 INFO @ Sat, 15 Jan 2022 22:38:16: 15000000 INFO @ Sat, 15 Jan 2022 22:38:17: 4000000 INFO @ Sat, 15 Jan 2022 22:38:22: 10000000 INFO @ Sat, 15 Jan 2022 22:38:23: 16000000 INFO @ Sat, 15 Jan 2022 22:38:24: 5000000 INFO @ Sat, 15 Jan 2022 22:38:29: 11000000 INFO @ Sat, 15 Jan 2022 22:38:29: 17000000 INFO @ Sat, 15 Jan 2022 22:38:30: 6000000 INFO @ Sat, 15 Jan 2022 22:38:35: 12000000 INFO @ Sat, 15 Jan 2022 22:38:36: 18000000 INFO @ Sat, 15 Jan 2022 22:38:37: 7000000 INFO @ Sat, 15 Jan 2022 22:38:42: 13000000 INFO @ Sat, 15 Jan 2022 22:38:43: 19000000 INFO @ Sat, 15 Jan 2022 22:38:44: 8000000 INFO @ Sat, 15 Jan 2022 22:38:48: 14000000 INFO @ Sat, 15 Jan 2022 22:38:49: 20000000 INFO @ Sat, 15 Jan 2022 22:38:50: 9000000 INFO @ Sat, 15 Jan 2022 22:38:55: 15000000 INFO @ Sat, 15 Jan 2022 22:38:56: 21000000 INFO @ Sat, 15 Jan 2022 22:38:57: 10000000 INFO @ Sat, 15 Jan 2022 22:38:58: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:38:58: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:38:58: #1 total tags in treatment: 7902453 INFO @ Sat, 15 Jan 2022 22:38:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:38:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:38:58: #1 tags after filtering in treatment: 6348623 INFO @ Sat, 15 Jan 2022 22:38:58: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 22:38:58: #1 finished! INFO @ Sat, 15 Jan 2022 22:38:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:38:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:38:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:38:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:38:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:39:02: 16000000 INFO @ Sat, 15 Jan 2022 22:39:03: 11000000 INFO @ Sat, 15 Jan 2022 22:39:08: 17000000 INFO @ Sat, 15 Jan 2022 22:39:09: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:39:14: 18000000 INFO @ Sat, 15 Jan 2022 22:39:16: 13000000 INFO @ Sat, 15 Jan 2022 22:39:22: 19000000 INFO @ Sat, 15 Jan 2022 22:39:22: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:39:28: 15000000 INFO @ Sat, 15 Jan 2022 22:39:29: 20000000 INFO @ Sat, 15 Jan 2022 22:39:34: 16000000 INFO @ Sat, 15 Jan 2022 22:39:35: 21000000 INFO @ Sat, 15 Jan 2022 22:39:37: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:39:37: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:39:37: #1 total tags in treatment: 7902453 INFO @ Sat, 15 Jan 2022 22:39:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:39:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:39:37: #1 tags after filtering in treatment: 6348623 INFO @ Sat, 15 Jan 2022 22:39:37: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 22:39:37: #1 finished! INFO @ Sat, 15 Jan 2022 22:39:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:39:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:39:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:39:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:39:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:39:40: 17000000 INFO @ Sat, 15 Jan 2022 22:39:46: 18000000 INFO @ Sat, 15 Jan 2022 22:39:52: 19000000 INFO @ Sat, 15 Jan 2022 22:39:57: 20000000 INFO @ Sat, 15 Jan 2022 22:40:03: 21000000 INFO @ Sat, 15 Jan 2022 22:40:05: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:40:05: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:40:05: #1 total tags in treatment: 7902453 INFO @ Sat, 15 Jan 2022 22:40:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:40:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:40:05: #1 tags after filtering in treatment: 6348623 INFO @ Sat, 15 Jan 2022 22:40:05: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 15 Jan 2022 22:40:05: #1 finished! INFO @ Sat, 15 Jan 2022 22:40:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:40:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:40:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:40:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:40:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786313/SRX9786313.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling