Job ID = 14522157 SRX = SRX9786312 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16606773 spots for SRR13362176/SRR13362176.sra Written 16606773 spots for SRR13362176/SRR13362176.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:35 16606773 reads; of these: 16606773 (100.00%) were paired; of these: 6892113 (41.50%) aligned concordantly 0 times 8965149 (53.98%) aligned concordantly exactly 1 time 749511 (4.51%) aligned concordantly >1 times ---- 6892113 pairs aligned concordantly 0 times; of these: 316129 (4.59%) aligned discordantly 1 time ---- 6575984 pairs aligned 0 times concordantly or discordantly; of these: 13151968 mates make up the pairs; of these: 7368111 (56.02%) aligned 0 times 5213021 (39.64%) aligned exactly 1 time 570836 (4.34%) aligned >1 times 77.82% overall alignment rate Time searching: 00:09:35 Overall time: 00:09:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 146116 / 10029487 = 0.0146 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:33:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:33:14: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:33:14: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:33:19: 1000000 INFO @ Sat, 15 Jan 2022 22:33:24: 2000000 INFO @ Sat, 15 Jan 2022 22:33:29: 3000000 INFO @ Sat, 15 Jan 2022 22:33:34: 4000000 INFO @ Sat, 15 Jan 2022 22:33:39: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:33:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:33:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:33:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:33:45: 6000000 INFO @ Sat, 15 Jan 2022 22:33:51: 1000000 INFO @ Sat, 15 Jan 2022 22:33:52: 7000000 INFO @ Sat, 15 Jan 2022 22:33:58: 8000000 INFO @ Sat, 15 Jan 2022 22:33:59: 2000000 INFO @ Sat, 15 Jan 2022 22:34:05: 9000000 INFO @ Sat, 15 Jan 2022 22:34:07: 3000000 INFO @ Sat, 15 Jan 2022 22:34:11: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:34:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:34:14: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:34:14: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:34:15: 4000000 INFO @ Sat, 15 Jan 2022 22:34:18: 11000000 INFO @ Sat, 15 Jan 2022 22:34:21: 1000000 INFO @ Sat, 15 Jan 2022 22:34:22: 5000000 INFO @ Sat, 15 Jan 2022 22:34:25: 12000000 INFO @ Sat, 15 Jan 2022 22:34:29: 2000000 INFO @ Sat, 15 Jan 2022 22:34:30: 6000000 INFO @ Sat, 15 Jan 2022 22:34:31: 13000000 INFO @ Sat, 15 Jan 2022 22:34:37: 3000000 INFO @ Sat, 15 Jan 2022 22:34:38: 7000000 INFO @ Sat, 15 Jan 2022 22:34:38: 14000000 INFO @ Sat, 15 Jan 2022 22:34:44: 4000000 INFO @ Sat, 15 Jan 2022 22:34:45: 15000000 INFO @ Sat, 15 Jan 2022 22:34:45: 8000000 INFO @ Sat, 15 Jan 2022 22:34:51: 16000000 INFO @ Sat, 15 Jan 2022 22:34:52: 5000000 INFO @ Sat, 15 Jan 2022 22:34:52: 9000000 INFO @ Sat, 15 Jan 2022 22:34:58: 17000000 INFO @ Sat, 15 Jan 2022 22:35:00: 6000000 INFO @ Sat, 15 Jan 2022 22:35:00: 10000000 INFO @ Sat, 15 Jan 2022 22:35:05: 18000000 INFO @ Sat, 15 Jan 2022 22:35:07: 11000000 INFO @ Sat, 15 Jan 2022 22:35:07: 7000000 INFO @ Sat, 15 Jan 2022 22:35:12: 19000000 INFO @ Sat, 15 Jan 2022 22:35:14: 12000000 INFO @ Sat, 15 Jan 2022 22:35:15: 8000000 INFO @ Sat, 15 Jan 2022 22:35:18: 20000000 INFO @ Sat, 15 Jan 2022 22:35:22: 13000000 INFO @ Sat, 15 Jan 2022 22:35:22: 9000000 INFO @ Sat, 15 Jan 2022 22:35:25: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:35:29: 14000000 INFO @ Sat, 15 Jan 2022 22:35:30: 10000000 INFO @ Sat, 15 Jan 2022 22:35:32: 22000000 INFO @ Sat, 15 Jan 2022 22:35:37: 15000000 INFO @ Sat, 15 Jan 2022 22:35:37: 11000000 INFO @ Sat, 15 Jan 2022 22:35:38: 23000000 INFO @ Sat, 15 Jan 2022 22:35:44: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:35:45: 12000000 INFO @ Sat, 15 Jan 2022 22:35:45: 24000000 INFO @ Sat, 15 Jan 2022 22:35:52: 25000000 INFO @ Sat, 15 Jan 2022 22:35:52: 17000000 INFO @ Sat, 15 Jan 2022 22:35:53: 13000000 INFO @ Sat, 15 Jan 2022 22:35:55: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:35:55: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:35:55: #1 total tags in treatment: 9569673 INFO @ Sat, 15 Jan 2022 22:35:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:35:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:35:56: #1 tags after filtering in treatment: 7359870 INFO @ Sat, 15 Jan 2022 22:35:56: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 22:35:56: #1 finished! INFO @ Sat, 15 Jan 2022 22:35:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:35:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:35:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:35:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:35:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:35:59: 18000000 INFO @ Sat, 15 Jan 2022 22:36:00: 14000000 INFO @ Sat, 15 Jan 2022 22:36:07: 19000000 INFO @ Sat, 15 Jan 2022 22:36:08: 15000000 INFO @ Sat, 15 Jan 2022 22:36:14: 20000000 INFO @ Sat, 15 Jan 2022 22:36:15: 16000000 INFO @ Sat, 15 Jan 2022 22:36:22: 21000000 INFO @ Sat, 15 Jan 2022 22:36:23: 17000000 INFO @ Sat, 15 Jan 2022 22:36:29: 22000000 INFO @ Sat, 15 Jan 2022 22:36:31: 18000000 INFO @ Sat, 15 Jan 2022 22:36:37: 23000000 INFO @ Sat, 15 Jan 2022 22:36:38: 19000000 INFO @ Sat, 15 Jan 2022 22:36:44: 24000000 INFO @ Sat, 15 Jan 2022 22:36:46: 20000000 INFO @ Sat, 15 Jan 2022 22:36:52: 25000000 INFO @ Sat, 15 Jan 2022 22:36:54: 21000000 INFO @ Sat, 15 Jan 2022 22:36:56: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:36:56: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:36:56: #1 total tags in treatment: 9569673 INFO @ Sat, 15 Jan 2022 22:36:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:36:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:36:56: #1 tags after filtering in treatment: 7359870 INFO @ Sat, 15 Jan 2022 22:36:56: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 22:36:56: #1 finished! INFO @ Sat, 15 Jan 2022 22:36:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:36:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:36:57: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:36:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:36:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:37:01: 22000000 INFO @ Sat, 15 Jan 2022 22:37:08: 23000000 INFO @ Sat, 15 Jan 2022 22:37:14: 24000000 INFO @ Sat, 15 Jan 2022 22:37:21: 25000000 INFO @ Sat, 15 Jan 2022 22:37:25: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:37:25: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:37:25: #1 total tags in treatment: 9569673 INFO @ Sat, 15 Jan 2022 22:37:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:37:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:37:25: #1 tags after filtering in treatment: 7359870 INFO @ Sat, 15 Jan 2022 22:37:25: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 22:37:25: #1 finished! INFO @ Sat, 15 Jan 2022 22:37:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:37:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:37:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:37:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:37:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786312/SRX9786312.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling