Job ID = 14522156 SRX = SRX9786311 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 12679341 spots for SRR13362175/SRR13362175.sra Written 12679341 spots for SRR13362175/SRR13362175.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:57 12679341 reads; of these: 12679341 (100.00%) were paired; of these: 1782219 (14.06%) aligned concordantly 0 times 9662433 (76.21%) aligned concordantly exactly 1 time 1234689 (9.74%) aligned concordantly >1 times ---- 1782219 pairs aligned concordantly 0 times; of these: 128281 (7.20%) aligned discordantly 1 time ---- 1653938 pairs aligned 0 times concordantly or discordantly; of these: 3307876 mates make up the pairs; of these: 1982160 (59.92%) aligned 0 times 1132747 (34.24%) aligned exactly 1 time 192969 (5.83%) aligned >1 times 92.18% overall alignment rate Time searching: 00:15:57 Overall time: 00:15:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 666953 / 11024448 = 0.0605 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:43:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:43:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:43:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:43:17: 1000000 INFO @ Sat, 15 Jan 2022 22:43:27: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:43:37: 3000000 INFO @ Sat, 15 Jan 2022 22:43:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:43:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:43:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:43:47: 1000000 INFO @ Sat, 15 Jan 2022 22:43:47: 4000000 INFO @ Sat, 15 Jan 2022 22:43:56: 2000000 INFO @ Sat, 15 Jan 2022 22:43:58: 5000000 INFO @ Sat, 15 Jan 2022 22:44:05: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:44:08: 6000000 INFO @ Sat, 15 Jan 2022 22:44:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:44:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:44:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:44:14: 4000000 INFO @ Sat, 15 Jan 2022 22:44:17: 1000000 INFO @ Sat, 15 Jan 2022 22:44:19: 7000000 INFO @ Sat, 15 Jan 2022 22:44:23: 5000000 INFO @ Sat, 15 Jan 2022 22:44:25: 2000000 INFO @ Sat, 15 Jan 2022 22:44:29: 8000000 INFO @ Sat, 15 Jan 2022 22:44:32: 6000000 INFO @ Sat, 15 Jan 2022 22:44:33: 3000000 INFO @ Sat, 15 Jan 2022 22:44:40: 9000000 INFO @ Sat, 15 Jan 2022 22:44:41: 7000000 INFO @ Sat, 15 Jan 2022 22:44:42: 4000000 INFO @ Sat, 15 Jan 2022 22:44:50: 8000000 INFO @ Sat, 15 Jan 2022 22:44:51: 10000000 INFO @ Sat, 15 Jan 2022 22:44:51: 5000000 INFO @ Sat, 15 Jan 2022 22:44:59: 6000000 INFO @ Sat, 15 Jan 2022 22:44:59: 9000000 INFO @ Sat, 15 Jan 2022 22:45:01: 11000000 INFO @ Sat, 15 Jan 2022 22:45:06: 7000000 INFO @ Sat, 15 Jan 2022 22:45:07: 10000000 INFO @ Sat, 15 Jan 2022 22:45:12: 12000000 INFO @ Sat, 15 Jan 2022 22:45:14: 8000000 INFO @ Sat, 15 Jan 2022 22:45:16: 11000000 INFO @ Sat, 15 Jan 2022 22:45:22: 9000000 INFO @ Sat, 15 Jan 2022 22:45:23: 13000000 INFO @ Sat, 15 Jan 2022 22:45:25: 12000000 INFO @ Sat, 15 Jan 2022 22:45:29: 10000000 INFO @ Sat, 15 Jan 2022 22:45:33: 14000000 INFO @ Sat, 15 Jan 2022 22:45:34: 13000000 INFO @ Sat, 15 Jan 2022 22:45:37: 11000000 INFO @ Sat, 15 Jan 2022 22:45:42: 14000000 INFO @ Sat, 15 Jan 2022 22:45:44: 15000000 INFO @ Sat, 15 Jan 2022 22:45:45: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:45:51: 15000000 INFO @ Sat, 15 Jan 2022 22:45:53: 13000000 INFO @ Sat, 15 Jan 2022 22:45:54: 16000000 INFO @ Sat, 15 Jan 2022 22:46:00: 16000000 INFO @ Sat, 15 Jan 2022 22:46:01: 14000000 INFO @ Sat, 15 Jan 2022 22:46:05: 17000000 INFO @ Sat, 15 Jan 2022 22:46:08: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:46:08: 17000000 INFO @ Sat, 15 Jan 2022 22:46:15: 18000000 INFO @ Sat, 15 Jan 2022 22:46:16: 16000000 INFO @ Sat, 15 Jan 2022 22:46:17: 18000000 INFO @ Sat, 15 Jan 2022 22:46:23: 17000000 INFO @ Sat, 15 Jan 2022 22:46:26: 19000000 INFO @ Sat, 15 Jan 2022 22:46:26: 19000000 INFO @ Sat, 15 Jan 2022 22:46:31: 18000000 INFO @ Sat, 15 Jan 2022 22:46:35: 20000000 INFO @ Sat, 15 Jan 2022 22:46:37: 20000000 INFO @ Sat, 15 Jan 2022 22:46:39: 19000000 INFO @ Sat, 15 Jan 2022 22:46:44: 21000000 INFO @ Sat, 15 Jan 2022 22:46:46: 20000000 INFO @ Sat, 15 Jan 2022 22:46:47: 21000000 INFO @ Sat, 15 Jan 2022 22:46:52: 22000000 INFO @ Sat, 15 Jan 2022 22:46:53: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 22:46:53: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 22:46:53: #1 total tags in treatment: 10233245 INFO @ Sat, 15 Jan 2022 22:46:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:46:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:46:53: #1 tags after filtering in treatment: 6397227 INFO @ Sat, 15 Jan 2022 22:46:53: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 22:46:53: #1 finished! INFO @ Sat, 15 Jan 2022 22:46:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:46:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:46:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:46:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:46:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:46:54: 21000000 INFO @ Sat, 15 Jan 2022 22:46:58: 22000000 INFO @ Sat, 15 Jan 2022 22:46:58: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 22:46:58: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 22:46:58: #1 total tags in treatment: 10233245 INFO @ Sat, 15 Jan 2022 22:46:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:46:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:46:59: #1 tags after filtering in treatment: 6397227 INFO @ Sat, 15 Jan 2022 22:46:59: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 22:46:59: #1 finished! INFO @ Sat, 15 Jan 2022 22:46:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:46:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:46:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:46:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:46:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:47:02: 22000000 INFO @ Sat, 15 Jan 2022 22:47:02: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 22:47:02: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 22:47:02: #1 total tags in treatment: 10233245 INFO @ Sat, 15 Jan 2022 22:47:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:47:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:47:02: #1 tags after filtering in treatment: 6397227 INFO @ Sat, 15 Jan 2022 22:47:02: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 22:47:02: #1 finished! INFO @ Sat, 15 Jan 2022 22:47:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:47:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:47:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:47:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:47:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786311/SRX9786311.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling