Job ID = 14522155 SRX = SRX9786310 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 12527375 spots for SRR13362174/SRR13362174.sra Written 12527375 spots for SRR13362174/SRR13362174.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:50 12527375 reads; of these: 12527375 (100.00%) were paired; of these: 1903869 (15.20%) aligned concordantly 0 times 9653843 (77.06%) aligned concordantly exactly 1 time 969663 (7.74%) aligned concordantly >1 times ---- 1903869 pairs aligned concordantly 0 times; of these: 166199 (8.73%) aligned discordantly 1 time ---- 1737670 pairs aligned 0 times concordantly or discordantly; of these: 3475340 mates make up the pairs; of these: 2125114 (61.15%) aligned 0 times 1177174 (33.87%) aligned exactly 1 time 173052 (4.98%) aligned >1 times 91.52% overall alignment rate Time searching: 00:14:50 Overall time: 00:14:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 878617 / 10788872 = 0.0814 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:41:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:41:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:41:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:42:06: 1000000 INFO @ Sat, 15 Jan 2022 22:42:14: 2000000 INFO @ Sat, 15 Jan 2022 22:42:22: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:42:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:42:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:42:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:42:30: 4000000 INFO @ Sat, 15 Jan 2022 22:42:35: 1000000 INFO @ Sat, 15 Jan 2022 22:42:38: 5000000 INFO @ Sat, 15 Jan 2022 22:42:43: 2000000 INFO @ Sat, 15 Jan 2022 22:42:46: 6000000 INFO @ Sat, 15 Jan 2022 22:42:52: 3000000 INFO @ Sat, 15 Jan 2022 22:42:54: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:42:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:42:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:42:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:43:00: 4000000 INFO @ Sat, 15 Jan 2022 22:43:02: 8000000 INFO @ Sat, 15 Jan 2022 22:43:06: 1000000 INFO @ Sat, 15 Jan 2022 22:43:08: 5000000 INFO @ Sat, 15 Jan 2022 22:43:10: 9000000 INFO @ Sat, 15 Jan 2022 22:43:14: 2000000 INFO @ Sat, 15 Jan 2022 22:43:16: 6000000 INFO @ Sat, 15 Jan 2022 22:43:18: 10000000 INFO @ Sat, 15 Jan 2022 22:43:23: 3000000 INFO @ Sat, 15 Jan 2022 22:43:24: 7000000 INFO @ Sat, 15 Jan 2022 22:43:27: 11000000 INFO @ Sat, 15 Jan 2022 22:43:31: 4000000 INFO @ Sat, 15 Jan 2022 22:43:32: 8000000 INFO @ Sat, 15 Jan 2022 22:43:35: 12000000 INFO @ Sat, 15 Jan 2022 22:43:39: 5000000 INFO @ Sat, 15 Jan 2022 22:43:40: 9000000 INFO @ Sat, 15 Jan 2022 22:43:43: 13000000 INFO @ Sat, 15 Jan 2022 22:43:47: 6000000 INFO @ Sat, 15 Jan 2022 22:43:48: 10000000 INFO @ Sat, 15 Jan 2022 22:43:52: 14000000 INFO @ Sat, 15 Jan 2022 22:43:55: 7000000 INFO @ Sat, 15 Jan 2022 22:43:56: 11000000 INFO @ Sat, 15 Jan 2022 22:44:00: 15000000 INFO @ Sat, 15 Jan 2022 22:44:03: 8000000 INFO @ Sat, 15 Jan 2022 22:44:04: 12000000 INFO @ Sat, 15 Jan 2022 22:44:09: 16000000 INFO @ Sat, 15 Jan 2022 22:44:12: 9000000 INFO @ Sat, 15 Jan 2022 22:44:13: 13000000 INFO @ Sat, 15 Jan 2022 22:44:17: 17000000 INFO @ Sat, 15 Jan 2022 22:44:20: 10000000 INFO @ Sat, 15 Jan 2022 22:44:21: 14000000 INFO @ Sat, 15 Jan 2022 22:44:27: 18000000 INFO @ Sat, 15 Jan 2022 22:44:28: 11000000 INFO @ Sat, 15 Jan 2022 22:44:30: 15000000 INFO @ Sat, 15 Jan 2022 22:44:36: 19000000 INFO @ Sat, 15 Jan 2022 22:44:37: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:44:40: 16000000 INFO @ Sat, 15 Jan 2022 22:44:44: 20000000 INFO @ Sat, 15 Jan 2022 22:44:45: 13000000 INFO @ Sat, 15 Jan 2022 22:44:49: 17000000 INFO @ Sat, 15 Jan 2022 22:44:53: 21000000 INFO @ Sat, 15 Jan 2022 22:44:54: 14000000 INFO @ Sat, 15 Jan 2022 22:44:54: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 22:44:54: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 22:44:54: #1 total tags in treatment: 9750191 INFO @ Sat, 15 Jan 2022 22:44:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:44:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:44:54: #1 tags after filtering in treatment: 5694873 INFO @ Sat, 15 Jan 2022 22:44:54: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 22:44:54: #1 finished! INFO @ Sat, 15 Jan 2022 22:44:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:44:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:44:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:44:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:44:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:44:58: 18000000 INFO @ Sat, 15 Jan 2022 22:45:02: 15000000 INFO @ Sat, 15 Jan 2022 22:45:07: 19000000 INFO @ Sat, 15 Jan 2022 22:45:10: 16000000 INFO @ Sat, 15 Jan 2022 22:45:16: 20000000 INFO @ Sat, 15 Jan 2022 22:45:19: 17000000 INFO @ Sat, 15 Jan 2022 22:45:24: 21000000 INFO @ Sat, 15 Jan 2022 22:45:26: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 22:45:26: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 22:45:26: #1 total tags in treatment: 9750191 INFO @ Sat, 15 Jan 2022 22:45:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:45:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:45:26: #1 tags after filtering in treatment: 5694873 INFO @ Sat, 15 Jan 2022 22:45:26: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 22:45:26: #1 finished! INFO @ Sat, 15 Jan 2022 22:45:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:45:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:45:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:45:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:45:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:45:28: 18000000 INFO @ Sat, 15 Jan 2022 22:45:36: 19000000 INFO @ Sat, 15 Jan 2022 22:45:44: 20000000 INFO @ Sat, 15 Jan 2022 22:45:53: 21000000 INFO @ Sat, 15 Jan 2022 22:45:54: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 22:45:54: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 22:45:54: #1 total tags in treatment: 9750191 INFO @ Sat, 15 Jan 2022 22:45:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:45:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:45:54: #1 tags after filtering in treatment: 5694873 INFO @ Sat, 15 Jan 2022 22:45:54: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 22:45:54: #1 finished! INFO @ Sat, 15 Jan 2022 22:45:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:45:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:45:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:45:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:45:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786310/SRX9786310.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling