Job ID = 14522133 SRX = SRX9786308 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16013718 spots for SRR13362172/SRR13362172.sra Written 16013718 spots for SRR13362172/SRR13362172.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:56 16013718 reads; of these: 16013718 (100.00%) were paired; of these: 2924736 (18.26%) aligned concordantly 0 times 12101893 (75.57%) aligned concordantly exactly 1 time 987089 (6.16%) aligned concordantly >1 times ---- 2924736 pairs aligned concordantly 0 times; of these: 391167 (13.37%) aligned discordantly 1 time ---- 2533569 pairs aligned 0 times concordantly or discordantly; of these: 5067138 mates make up the pairs; of these: 3123060 (61.63%) aligned 0 times 1702084 (33.59%) aligned exactly 1 time 241994 (4.78%) aligned >1 times 90.25% overall alignment rate Time searching: 00:12:56 Overall time: 00:12:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1678082 / 13479100 = 0.1245 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:36:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:36:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:36:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:36:33: 1000000 INFO @ Sat, 15 Jan 2022 22:36:40: 2000000 INFO @ Sat, 15 Jan 2022 22:36:47: 3000000 INFO @ Sat, 15 Jan 2022 22:36:54: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:36:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:36:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:36:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:37:01: 5000000 INFO @ Sat, 15 Jan 2022 22:37:02: 1000000 INFO @ Sat, 15 Jan 2022 22:37:08: 6000000 INFO @ Sat, 15 Jan 2022 22:37:09: 2000000 INFO @ Sat, 15 Jan 2022 22:37:15: 7000000 INFO @ Sat, 15 Jan 2022 22:37:15: 3000000 INFO @ Sat, 15 Jan 2022 22:37:22: 4000000 INFO @ Sat, 15 Jan 2022 22:37:22: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:37:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:37:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:37:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:37:28: 5000000 INFO @ Sat, 15 Jan 2022 22:37:29: 9000000 INFO @ Sat, 15 Jan 2022 22:37:33: 1000000 INFO @ Sat, 15 Jan 2022 22:37:35: 6000000 INFO @ Sat, 15 Jan 2022 22:37:36: 10000000 INFO @ Sat, 15 Jan 2022 22:37:40: 2000000 INFO @ Sat, 15 Jan 2022 22:37:41: 7000000 INFO @ Sat, 15 Jan 2022 22:37:44: 11000000 INFO @ Sat, 15 Jan 2022 22:37:47: 3000000 INFO @ Sat, 15 Jan 2022 22:37:47: 8000000 INFO @ Sat, 15 Jan 2022 22:37:51: 12000000 INFO @ Sat, 15 Jan 2022 22:37:54: 4000000 INFO @ Sat, 15 Jan 2022 22:37:54: 9000000 INFO @ Sat, 15 Jan 2022 22:37:58: 13000000 INFO @ Sat, 15 Jan 2022 22:38:00: 10000000 INFO @ Sat, 15 Jan 2022 22:38:00: 5000000 INFO @ Sat, 15 Jan 2022 22:38:06: 14000000 INFO @ Sat, 15 Jan 2022 22:38:07: 11000000 INFO @ Sat, 15 Jan 2022 22:38:07: 6000000 INFO @ Sat, 15 Jan 2022 22:38:13: 12000000 INFO @ Sat, 15 Jan 2022 22:38:13: 15000000 INFO @ Sat, 15 Jan 2022 22:38:14: 7000000 INFO @ Sat, 15 Jan 2022 22:38:20: 13000000 INFO @ Sat, 15 Jan 2022 22:38:21: 8000000 INFO @ Sat, 15 Jan 2022 22:38:21: 16000000 INFO @ Sat, 15 Jan 2022 22:38:26: 14000000 INFO @ Sat, 15 Jan 2022 22:38:27: 9000000 INFO @ Sat, 15 Jan 2022 22:38:29: 17000000 INFO @ Sat, 15 Jan 2022 22:38:33: 15000000 INFO @ Sat, 15 Jan 2022 22:38:34: 10000000 INFO @ Sat, 15 Jan 2022 22:38:36: 18000000 INFO @ Sat, 15 Jan 2022 22:38:39: 16000000 INFO @ Sat, 15 Jan 2022 22:38:41: 11000000 INFO @ Sat, 15 Jan 2022 22:38:43: 19000000 INFO @ Sat, 15 Jan 2022 22:38:46: 17000000 INFO @ Sat, 15 Jan 2022 22:38:48: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:38:50: 20000000 INFO @ Sat, 15 Jan 2022 22:38:53: 18000000 INFO @ Sat, 15 Jan 2022 22:38:55: 13000000 INFO @ Sat, 15 Jan 2022 22:38:58: 21000000 INFO @ Sat, 15 Jan 2022 22:38:59: 19000000 INFO @ Sat, 15 Jan 2022 22:39:01: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:39:05: 22000000 INFO @ Sat, 15 Jan 2022 22:39:06: 20000000 INFO @ Sat, 15 Jan 2022 22:39:08: 15000000 INFO @ Sat, 15 Jan 2022 22:39:13: 21000000 INFO @ Sat, 15 Jan 2022 22:39:13: 23000000 INFO @ Sat, 15 Jan 2022 22:39:15: 16000000 INFO @ Sat, 15 Jan 2022 22:39:19: 22000000 INFO @ Sat, 15 Jan 2022 22:39:20: 24000000 INFO @ Sat, 15 Jan 2022 22:39:22: 17000000 INFO @ Sat, 15 Jan 2022 22:39:26: 23000000 INFO @ Sat, 15 Jan 2022 22:39:27: 25000000 INFO @ Sat, 15 Jan 2022 22:39:29: 18000000 INFO @ Sat, 15 Jan 2022 22:39:31: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 22:39:31: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 22:39:31: #1 total tags in treatment: 11433567 INFO @ Sat, 15 Jan 2022 22:39:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:39:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:39:31: #1 tags after filtering in treatment: 6214843 INFO @ Sat, 15 Jan 2022 22:39:31: #1 Redundant rate of treatment: 0.46 INFO @ Sat, 15 Jan 2022 22:39:31: #1 finished! INFO @ Sat, 15 Jan 2022 22:39:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:39:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:39:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:39:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:39:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:39:33: 24000000 INFO @ Sat, 15 Jan 2022 22:39:36: 19000000 INFO @ Sat, 15 Jan 2022 22:39:39: 25000000 INFO @ Sat, 15 Jan 2022 22:39:42: 20000000 INFO @ Sat, 15 Jan 2022 22:39:43: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 22:39:43: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 22:39:43: #1 total tags in treatment: 11433567 INFO @ Sat, 15 Jan 2022 22:39:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:39:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:39:43: #1 tags after filtering in treatment: 6214843 INFO @ Sat, 15 Jan 2022 22:39:43: #1 Redundant rate of treatment: 0.46 INFO @ Sat, 15 Jan 2022 22:39:43: #1 finished! INFO @ Sat, 15 Jan 2022 22:39:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:39:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:39:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:39:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:39:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:39:49: 21000000 INFO @ Sat, 15 Jan 2022 22:39:55: 22000000 INFO @ Sat, 15 Jan 2022 22:40:02: 23000000 INFO @ Sat, 15 Jan 2022 22:40:08: 24000000 INFO @ Sat, 15 Jan 2022 22:40:14: 25000000 INFO @ Sat, 15 Jan 2022 22:40:18: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 22:40:18: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 22:40:18: #1 total tags in treatment: 11433567 INFO @ Sat, 15 Jan 2022 22:40:18: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:40:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:40:18: #1 tags after filtering in treatment: 6214843 INFO @ Sat, 15 Jan 2022 22:40:18: #1 Redundant rate of treatment: 0.46 INFO @ Sat, 15 Jan 2022 22:40:18: #1 finished! INFO @ Sat, 15 Jan 2022 22:40:18: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:40:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:40:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:40:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:40:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786308/SRX9786308.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling