Job ID = 14522126 SRX = SRX9786301 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16270063 spots for SRR13362133/SRR13362133.sra Written 16270063 spots for SRR13362133/SRR13362133.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:19 16270063 reads; of these: 16270063 (100.00%) were paired; of these: 6922328 (42.55%) aligned concordantly 0 times 7723282 (47.47%) aligned concordantly exactly 1 time 1624453 (9.98%) aligned concordantly >1 times ---- 6922328 pairs aligned concordantly 0 times; of these: 109436 (1.58%) aligned discordantly 1 time ---- 6812892 pairs aligned 0 times concordantly or discordantly; of these: 13625784 mates make up the pairs; of these: 8402159 (61.66%) aligned 0 times 4283219 (31.43%) aligned exactly 1 time 940406 (6.90%) aligned >1 times 74.18% overall alignment rate Time searching: 00:13:19 Overall time: 00:13:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 742189 / 9453387 = 0.0785 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:37:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:37:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:37:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:37:36: 1000000 INFO @ Sat, 15 Jan 2022 22:37:41: 2000000 INFO @ Sat, 15 Jan 2022 22:37:46: 3000000 INFO @ Sat, 15 Jan 2022 22:37:51: 4000000 INFO @ Sat, 15 Jan 2022 22:37:55: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:38:00: 6000000 INFO @ Sat, 15 Jan 2022 22:38:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:38:01: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:38:01: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:38:05: 7000000 INFO @ Sat, 15 Jan 2022 22:38:07: 1000000 INFO @ Sat, 15 Jan 2022 22:38:09: 8000000 INFO @ Sat, 15 Jan 2022 22:38:13: 2000000 INFO @ Sat, 15 Jan 2022 22:38:14: 9000000 INFO @ Sat, 15 Jan 2022 22:38:19: 3000000 INFO @ Sat, 15 Jan 2022 22:38:19: 10000000 INFO @ Sat, 15 Jan 2022 22:38:23: 11000000 INFO @ Sat, 15 Jan 2022 22:38:25: 4000000 INFO @ Sat, 15 Jan 2022 22:38:28: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:38:31: 5000000 INFO @ Sat, 15 Jan 2022 22:38:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:38:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:38:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:38:33: 13000000 INFO @ Sat, 15 Jan 2022 22:38:36: 1000000 INFO @ Sat, 15 Jan 2022 22:38:37: 6000000 INFO @ Sat, 15 Jan 2022 22:38:38: 14000000 INFO @ Sat, 15 Jan 2022 22:38:41: 2000000 INFO @ Sat, 15 Jan 2022 22:38:43: 7000000 INFO @ Sat, 15 Jan 2022 22:38:43: 15000000 INFO @ Sat, 15 Jan 2022 22:38:47: 3000000 INFO @ Sat, 15 Jan 2022 22:38:49: 16000000 INFO @ Sat, 15 Jan 2022 22:38:49: 8000000 INFO @ Sat, 15 Jan 2022 22:38:52: 4000000 INFO @ Sat, 15 Jan 2022 22:38:54: 17000000 INFO @ Sat, 15 Jan 2022 22:38:55: 9000000 INFO @ Sat, 15 Jan 2022 22:38:57: 5000000 INFO @ Sat, 15 Jan 2022 22:38:59: 18000000 INFO @ Sat, 15 Jan 2022 22:39:01: 10000000 INFO @ Sat, 15 Jan 2022 22:39:03: 6000000 INFO @ Sat, 15 Jan 2022 22:39:04: 19000000 INFO @ Sat, 15 Jan 2022 22:39:06: 11000000 INFO @ Sat, 15 Jan 2022 22:39:08: 7000000 INFO @ Sat, 15 Jan 2022 22:39:10: 20000000 INFO @ Sat, 15 Jan 2022 22:39:12: 12000000 INFO @ Sat, 15 Jan 2022 22:39:13: 8000000 INFO @ Sat, 15 Jan 2022 22:39:15: 21000000 INFO @ Sat, 15 Jan 2022 22:39:18: 13000000 INFO @ Sat, 15 Jan 2022 22:39:19: 9000000 INFO @ Sat, 15 Jan 2022 22:39:21: 22000000 INFO @ Sat, 15 Jan 2022 22:39:23: 14000000 INFO @ Sat, 15 Jan 2022 22:39:25: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:39:25: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:39:25: #1 total tags in treatment: 8606551 INFO @ Sat, 15 Jan 2022 22:39:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:39:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:39:25: 10000000 INFO @ Sat, 15 Jan 2022 22:39:25: #1 tags after filtering in treatment: 5212930 INFO @ Sat, 15 Jan 2022 22:39:25: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 22:39:25: #1 finished! INFO @ Sat, 15 Jan 2022 22:39:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:39:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:39:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:39:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:39:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:39:28: 15000000 INFO @ Sat, 15 Jan 2022 22:39:30: 11000000 INFO @ Sat, 15 Jan 2022 22:39:34: 16000000 INFO @ Sat, 15 Jan 2022 22:39:34: 12000000 INFO @ Sat, 15 Jan 2022 22:39:39: 17000000 INFO @ Sat, 15 Jan 2022 22:39:39: 13000000 INFO @ Sat, 15 Jan 2022 22:39:44: 14000000 INFO @ Sat, 15 Jan 2022 22:39:45: 18000000 INFO @ Sat, 15 Jan 2022 22:39:49: 15000000 INFO @ Sat, 15 Jan 2022 22:39:50: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:39:54: 16000000 INFO @ Sat, 15 Jan 2022 22:39:56: 20000000 INFO @ Sat, 15 Jan 2022 22:39:59: 17000000 INFO @ Sat, 15 Jan 2022 22:40:01: 21000000 INFO @ Sat, 15 Jan 2022 22:40:03: 18000000 INFO @ Sat, 15 Jan 2022 22:40:06: 22000000 INFO @ Sat, 15 Jan 2022 22:40:08: 19000000 INFO @ Sat, 15 Jan 2022 22:40:09: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:40:09: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:40:09: #1 total tags in treatment: 8606551 INFO @ Sat, 15 Jan 2022 22:40:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:40:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:40:10: #1 tags after filtering in treatment: 5212930 INFO @ Sat, 15 Jan 2022 22:40:10: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 22:40:10: #1 finished! INFO @ Sat, 15 Jan 2022 22:40:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:40:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:40:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:40:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:40:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:40:13: 20000000 INFO @ Sat, 15 Jan 2022 22:40:18: 21000000 INFO @ Sat, 15 Jan 2022 22:40:22: 22000000 INFO @ Sat, 15 Jan 2022 22:40:25: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:40:25: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:40:25: #1 total tags in treatment: 8606551 INFO @ Sat, 15 Jan 2022 22:40:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:40:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:40:26: #1 tags after filtering in treatment: 5212930 INFO @ Sat, 15 Jan 2022 22:40:26: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 22:40:26: #1 finished! INFO @ Sat, 15 Jan 2022 22:40:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:40:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:40:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:40:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:40:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786301/SRX9786301.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling