Job ID = 14522125 SRX = SRX9786300 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 35531709 spots for SRR13362132/SRR13362132.sra Written 35531709 spots for SRR13362132/SRR13362132.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:51 35531709 reads; of these: 35531709 (100.00%) were paired; of these: 14196377 (39.95%) aligned concordantly 0 times 17245546 (48.54%) aligned concordantly exactly 1 time 4089786 (11.51%) aligned concordantly >1 times ---- 14196377 pairs aligned concordantly 0 times; of these: 44467 (0.31%) aligned discordantly 1 time ---- 14151910 pairs aligned 0 times concordantly or discordantly; of these: 28303820 mates make up the pairs; of these: 16457739 (58.15%) aligned 0 times 9618944 (33.98%) aligned exactly 1 time 2227137 (7.87%) aligned >1 times 76.84% overall alignment rate Time searching: 00:22:51 Overall time: 00:22:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3093867 / 21371973 = 0.1448 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:54:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:54:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:54:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:54:12: 1000000 INFO @ Sat, 15 Jan 2022 22:54:17: 2000000 INFO @ Sat, 15 Jan 2022 22:54:22: 3000000 INFO @ Sat, 15 Jan 2022 22:54:27: 4000000 INFO @ Sat, 15 Jan 2022 22:54:32: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:54:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:54:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:54:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:54:37: 6000000 INFO @ Sat, 15 Jan 2022 22:54:44: 7000000 INFO @ Sat, 15 Jan 2022 22:54:44: 1000000 INFO @ Sat, 15 Jan 2022 22:54:50: 8000000 INFO @ Sat, 15 Jan 2022 22:54:51: 2000000 INFO @ Sat, 15 Jan 2022 22:54:56: 9000000 INFO @ Sat, 15 Jan 2022 22:54:57: 3000000 INFO @ Sat, 15 Jan 2022 22:55:02: 10000000 INFO @ Sat, 15 Jan 2022 22:55:04: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:55:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:55:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:55:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:55:09: 11000000 INFO @ Sat, 15 Jan 2022 22:55:11: 5000000 INFO @ Sat, 15 Jan 2022 22:55:14: 1000000 INFO @ Sat, 15 Jan 2022 22:55:15: 12000000 INFO @ Sat, 15 Jan 2022 22:55:17: 6000000 INFO @ Sat, 15 Jan 2022 22:55:20: 2000000 INFO @ Sat, 15 Jan 2022 22:55:21: 13000000 INFO @ Sat, 15 Jan 2022 22:55:24: 7000000 INFO @ Sat, 15 Jan 2022 22:55:26: 3000000 INFO @ Sat, 15 Jan 2022 22:55:28: 14000000 INFO @ Sat, 15 Jan 2022 22:55:31: 8000000 INFO @ Sat, 15 Jan 2022 22:55:33: 4000000 INFO @ Sat, 15 Jan 2022 22:55:34: 15000000 INFO @ Sat, 15 Jan 2022 22:55:38: 9000000 INFO @ Sat, 15 Jan 2022 22:55:39: 5000000 INFO @ Sat, 15 Jan 2022 22:55:41: 16000000 INFO @ Sat, 15 Jan 2022 22:55:44: 10000000 INFO @ Sat, 15 Jan 2022 22:55:45: 6000000 INFO @ Sat, 15 Jan 2022 22:55:48: 17000000 INFO @ Sat, 15 Jan 2022 22:55:51: 11000000 INFO @ Sat, 15 Jan 2022 22:55:51: 7000000 INFO @ Sat, 15 Jan 2022 22:55:55: 18000000 INFO @ Sat, 15 Jan 2022 22:55:57: 8000000 INFO @ Sat, 15 Jan 2022 22:55:57: 12000000 INFO @ Sat, 15 Jan 2022 22:56:02: 19000000 INFO @ Sat, 15 Jan 2022 22:56:04: 9000000 INFO @ Sat, 15 Jan 2022 22:56:04: 13000000 INFO @ Sat, 15 Jan 2022 22:56:09: 20000000 INFO @ Sat, 15 Jan 2022 22:56:10: 10000000 INFO @ Sat, 15 Jan 2022 22:56:11: 14000000 INFO @ Sat, 15 Jan 2022 22:56:15: 21000000 INFO @ Sat, 15 Jan 2022 22:56:16: 11000000 INFO @ Sat, 15 Jan 2022 22:56:17: 15000000 INFO @ Sat, 15 Jan 2022 22:56:21: 22000000 INFO @ Sat, 15 Jan 2022 22:56:23: 12000000 INFO @ Sat, 15 Jan 2022 22:56:23: 16000000 INFO @ Sat, 15 Jan 2022 22:56:28: 23000000 INFO @ Sat, 15 Jan 2022 22:56:29: 13000000 INFO @ Sat, 15 Jan 2022 22:56:29: 17000000 INFO @ Sat, 15 Jan 2022 22:56:34: 24000000 INFO @ Sat, 15 Jan 2022 22:56:36: 18000000 INFO @ Sat, 15 Jan 2022 22:56:36: 14000000 INFO @ Sat, 15 Jan 2022 22:56:41: 25000000 INFO @ Sat, 15 Jan 2022 22:56:42: 19000000 INFO @ Sat, 15 Jan 2022 22:56:42: 15000000 INFO @ Sat, 15 Jan 2022 22:56:47: 26000000 INFO @ Sat, 15 Jan 2022 22:56:49: 20000000 INFO @ Sat, 15 Jan 2022 22:56:49: 16000000 INFO @ Sat, 15 Jan 2022 22:56:53: 27000000 INFO @ Sat, 15 Jan 2022 22:56:55: 21000000 INFO @ Sat, 15 Jan 2022 22:56:56: 17000000 INFO @ Sat, 15 Jan 2022 22:56:59: 28000000 INFO @ Sat, 15 Jan 2022 22:57:02: 22000000 INFO @ Sat, 15 Jan 2022 22:57:03: 18000000 INFO @ Sat, 15 Jan 2022 22:57:05: 29000000 INFO @ Sat, 15 Jan 2022 22:57:09: 23000000 INFO @ Sat, 15 Jan 2022 22:57:10: 19000000 INFO @ Sat, 15 Jan 2022 22:57:12: 30000000 INFO @ Sat, 15 Jan 2022 22:57:16: 24000000 INFO @ Sat, 15 Jan 2022 22:57:16: 20000000 INFO @ Sat, 15 Jan 2022 22:57:18: 31000000 INFO @ Sat, 15 Jan 2022 22:57:22: 25000000 INFO @ Sat, 15 Jan 2022 22:57:22: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:57:24: 32000000 INFO @ Sat, 15 Jan 2022 22:57:29: 22000000 INFO @ Sat, 15 Jan 2022 22:57:29: 26000000 INFO @ Sat, 15 Jan 2022 22:57:30: 33000000 INFO @ Sat, 15 Jan 2022 22:57:36: 23000000 INFO @ Sat, 15 Jan 2022 22:57:36: 27000000 INFO @ Sat, 15 Jan 2022 22:57:37: 34000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:57:42: 24000000 INFO @ Sat, 15 Jan 2022 22:57:43: 28000000 INFO @ Sat, 15 Jan 2022 22:57:44: 35000000 INFO @ Sat, 15 Jan 2022 22:57:49: 25000000 INFO @ Sat, 15 Jan 2022 22:57:49: 29000000 INFO @ Sat, 15 Jan 2022 22:57:50: 36000000 INFO @ Sat, 15 Jan 2022 22:57:55: 26000000 INFO @ Sat, 15 Jan 2022 22:57:56: 30000000 INFO @ Sat, 15 Jan 2022 22:57:57: 37000000 INFO @ Sat, 15 Jan 2022 22:58:02: 27000000 INFO @ Sat, 15 Jan 2022 22:58:02: 31000000 INFO @ Sat, 15 Jan 2022 22:58:03: 38000000 INFO @ Sat, 15 Jan 2022 22:58:08: 28000000 INFO @ Sat, 15 Jan 2022 22:58:09: 32000000 INFO @ Sat, 15 Jan 2022 22:58:10: 39000000 INFO @ Sat, 15 Jan 2022 22:58:15: 29000000 INFO @ Sat, 15 Jan 2022 22:58:15: 33000000 INFO @ Sat, 15 Jan 2022 22:58:16: 40000000 INFO @ Sat, 15 Jan 2022 22:58:21: 30000000 INFO @ Sat, 15 Jan 2022 22:58:22: 34000000 INFO @ Sat, 15 Jan 2022 22:58:22: 41000000 INFO @ Sat, 15 Jan 2022 22:58:27: 31000000 INFO @ Sat, 15 Jan 2022 22:58:29: 42000000 INFO @ Sat, 15 Jan 2022 22:58:29: 35000000 INFO @ Sat, 15 Jan 2022 22:58:33: 32000000 INFO @ Sat, 15 Jan 2022 22:58:35: 43000000 INFO @ Sat, 15 Jan 2022 22:58:36: 36000000 INFO @ Sat, 15 Jan 2022 22:58:39: 33000000 INFO @ Sat, 15 Jan 2022 22:58:41: 44000000 INFO @ Sat, 15 Jan 2022 22:58:42: 37000000 INFO @ Sat, 15 Jan 2022 22:58:46: 34000000 INFO @ Sat, 15 Jan 2022 22:58:48: 45000000 INFO @ Sat, 15 Jan 2022 22:58:49: 38000000 INFO @ Sat, 15 Jan 2022 22:58:52: 35000000 INFO @ Sat, 15 Jan 2022 22:58:54: 46000000 INFO @ Sat, 15 Jan 2022 22:58:56: 39000000 INFO @ Sat, 15 Jan 2022 22:58:59: 36000000 INFO @ Sat, 15 Jan 2022 22:59:01: 47000000 INFO @ Sat, 15 Jan 2022 22:59:02: 40000000 INFO @ Sat, 15 Jan 2022 22:59:05: 37000000 INFO @ Sat, 15 Jan 2022 22:59:07: 48000000 INFO @ Sat, 15 Jan 2022 22:59:09: 41000000 INFO @ Sat, 15 Jan 2022 22:59:10: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:59:10: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:59:10: #1 total tags in treatment: 18241641 INFO @ Sat, 15 Jan 2022 22:59:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:59:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:59:11: #1 tags after filtering in treatment: 6980343 INFO @ Sat, 15 Jan 2022 22:59:11: #1 Redundant rate of treatment: 0.62 INFO @ Sat, 15 Jan 2022 22:59:11: #1 finished! INFO @ Sat, 15 Jan 2022 22:59:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:59:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:59:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:59:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:59:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:59:12: 38000000 INFO @ Sat, 15 Jan 2022 22:59:16: 42000000 INFO @ Sat, 15 Jan 2022 22:59:18: 39000000 INFO @ Sat, 15 Jan 2022 22:59:22: 43000000 INFO @ Sat, 15 Jan 2022 22:59:24: 40000000 INFO @ Sat, 15 Jan 2022 22:59:29: 44000000 INFO @ Sat, 15 Jan 2022 22:59:30: 41000000 INFO @ Sat, 15 Jan 2022 22:59:36: 45000000 INFO @ Sat, 15 Jan 2022 22:59:37: 42000000 INFO @ Sat, 15 Jan 2022 22:59:42: 46000000 INFO @ Sat, 15 Jan 2022 22:59:43: 43000000 INFO @ Sat, 15 Jan 2022 22:59:49: 47000000 INFO @ Sat, 15 Jan 2022 22:59:49: 44000000 INFO @ Sat, 15 Jan 2022 22:59:55: 45000000 INFO @ Sat, 15 Jan 2022 22:59:56: 48000000 INFO @ Sat, 15 Jan 2022 22:59:58: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 22:59:58: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 22:59:58: #1 total tags in treatment: 18241641 INFO @ Sat, 15 Jan 2022 22:59:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:59:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:59:59: #1 tags after filtering in treatment: 6980343 INFO @ Sat, 15 Jan 2022 22:59:59: #1 Redundant rate of treatment: 0.62 INFO @ Sat, 15 Jan 2022 22:59:59: #1 finished! INFO @ Sat, 15 Jan 2022 22:59:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:59:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:59:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:59:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:59:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 23:00:01: 46000000 INFO @ Sat, 15 Jan 2022 23:00:07: 47000000 INFO @ Sat, 15 Jan 2022 23:00:12: 48000000 INFO @ Sat, 15 Jan 2022 23:00:14: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 23:00:14: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 23:00:14: #1 total tags in treatment: 18241641 INFO @ Sat, 15 Jan 2022 23:00:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 23:00:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 23:00:15: #1 tags after filtering in treatment: 6980343 INFO @ Sat, 15 Jan 2022 23:00:15: #1 Redundant rate of treatment: 0.62 INFO @ Sat, 15 Jan 2022 23:00:15: #1 finished! INFO @ Sat, 15 Jan 2022 23:00:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 23:00:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 23:00:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 23:00:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 23:00:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786300/SRX9786300.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling