Job ID = 14519883 SRX = SRX9786299 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18418944 spots for SRR13362131/SRR13362131.sra Written 18418944 spots for SRR13362131/SRR13362131.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:56 18418944 reads; of these: 18418944 (100.00%) were paired; of these: 7347319 (39.89%) aligned concordantly 0 times 8629723 (46.85%) aligned concordantly exactly 1 time 2441902 (13.26%) aligned concordantly >1 times ---- 7347319 pairs aligned concordantly 0 times; of these: 14391 (0.20%) aligned discordantly 1 time ---- 7332928 pairs aligned 0 times concordantly or discordantly; of these: 14665856 mates make up the pairs; of these: 8647818 (58.97%) aligned 0 times 4729637 (32.25%) aligned exactly 1 time 1288401 (8.79%) aligned >1 times 76.52% overall alignment rate Time searching: 00:10:56 Overall time: 00:10:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1458165 / 11079558 = 0.1316 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:17:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:17:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:17:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:17:18: 1000000 INFO @ Sat, 15 Jan 2022 18:17:25: 2000000 INFO @ Sat, 15 Jan 2022 18:17:31: 3000000 INFO @ Sat, 15 Jan 2022 18:17:39: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:17:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:17:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:17:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:17:46: 5000000 INFO @ Sat, 15 Jan 2022 18:17:48: 1000000 INFO @ Sat, 15 Jan 2022 18:17:53: 6000000 INFO @ Sat, 15 Jan 2022 18:17:55: 2000000 INFO @ Sat, 15 Jan 2022 18:18:02: 7000000 INFO @ Sat, 15 Jan 2022 18:18:03: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:18:09: 8000000 INFO @ Sat, 15 Jan 2022 18:18:10: 4000000 INFO @ Sat, 15 Jan 2022 18:18:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:18:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:18:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:18:17: 9000000 INFO @ Sat, 15 Jan 2022 18:18:18: 5000000 INFO @ Sat, 15 Jan 2022 18:18:18: 1000000 INFO @ Sat, 15 Jan 2022 18:18:25: 10000000 INFO @ Sat, 15 Jan 2022 18:18:26: 6000000 INFO @ Sat, 15 Jan 2022 18:18:26: 2000000 INFO @ Sat, 15 Jan 2022 18:18:33: 7000000 INFO @ Sat, 15 Jan 2022 18:18:34: 3000000 INFO @ Sat, 15 Jan 2022 18:18:37: 11000000 INFO @ Sat, 15 Jan 2022 18:18:41: 8000000 INFO @ Sat, 15 Jan 2022 18:18:42: 4000000 INFO @ Sat, 15 Jan 2022 18:18:47: 12000000 INFO @ Sat, 15 Jan 2022 18:18:49: 9000000 INFO @ Sat, 15 Jan 2022 18:18:49: 5000000 INFO @ Sat, 15 Jan 2022 18:18:55: 13000000 INFO @ Sat, 15 Jan 2022 18:18:56: 10000000 INFO @ Sat, 15 Jan 2022 18:18:57: 6000000 INFO @ Sat, 15 Jan 2022 18:19:03: 14000000 INFO @ Sat, 15 Jan 2022 18:19:04: 11000000 INFO @ Sat, 15 Jan 2022 18:19:04: 7000000 INFO @ Sat, 15 Jan 2022 18:19:11: 15000000 INFO @ Sat, 15 Jan 2022 18:19:11: 12000000 INFO @ Sat, 15 Jan 2022 18:19:12: 8000000 INFO @ Sat, 15 Jan 2022 18:19:19: 16000000 INFO @ Sat, 15 Jan 2022 18:19:19: 13000000 INFO @ Sat, 15 Jan 2022 18:19:19: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:19:26: 17000000 INFO @ Sat, 15 Jan 2022 18:19:27: 14000000 INFO @ Sat, 15 Jan 2022 18:19:27: 10000000 INFO @ Sat, 15 Jan 2022 18:19:34: 18000000 INFO @ Sat, 15 Jan 2022 18:19:35: 11000000 INFO @ Sat, 15 Jan 2022 18:19:35: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:19:42: 19000000 INFO @ Sat, 15 Jan 2022 18:19:42: 12000000 INFO @ Sat, 15 Jan 2022 18:19:42: 16000000 INFO @ Sat, 15 Jan 2022 18:19:50: 20000000 INFO @ Sat, 15 Jan 2022 18:19:50: 17000000 INFO @ Sat, 15 Jan 2022 18:19:50: 13000000 INFO @ Sat, 15 Jan 2022 18:19:58: 21000000 INFO @ Sat, 15 Jan 2022 18:19:58: 18000000 INFO @ Sat, 15 Jan 2022 18:19:58: 14000000 INFO @ Sat, 15 Jan 2022 18:20:05: 22000000 INFO @ Sat, 15 Jan 2022 18:20:05: 15000000 INFO @ Sat, 15 Jan 2022 18:20:06: 19000000 INFO @ Sat, 15 Jan 2022 18:20:13: 16000000 INFO @ Sat, 15 Jan 2022 18:20:13: 23000000 INFO @ Sat, 15 Jan 2022 18:20:14: 20000000 INFO @ Sat, 15 Jan 2022 18:20:21: 17000000 INFO @ Sat, 15 Jan 2022 18:20:21: 24000000 INFO @ Sat, 15 Jan 2022 18:20:21: 21000000 INFO @ Sat, 15 Jan 2022 18:20:28: 18000000 INFO @ Sat, 15 Jan 2022 18:20:29: 25000000 INFO @ Sat, 15 Jan 2022 18:20:29: 22000000 INFO @ Sat, 15 Jan 2022 18:20:31: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:20:31: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:20:31: #1 total tags in treatment: 9613504 INFO @ Sat, 15 Jan 2022 18:20:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:20:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:20:31: #1 tags after filtering in treatment: 4509984 INFO @ Sat, 15 Jan 2022 18:20:31: #1 Redundant rate of treatment: 0.53 INFO @ Sat, 15 Jan 2022 18:20:31: #1 finished! INFO @ Sat, 15 Jan 2022 18:20:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:20:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:20:31: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:20:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:20:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:20:36: 19000000 INFO @ Sat, 15 Jan 2022 18:20:37: 23000000 INFO @ Sat, 15 Jan 2022 18:20:44: 20000000 INFO @ Sat, 15 Jan 2022 18:20:44: 24000000 INFO @ Sat, 15 Jan 2022 18:20:51: 21000000 INFO @ Sat, 15 Jan 2022 18:20:52: 25000000 INFO @ Sat, 15 Jan 2022 18:20:54: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:20:54: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:20:54: #1 total tags in treatment: 9613504 INFO @ Sat, 15 Jan 2022 18:20:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:20:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:20:54: #1 tags after filtering in treatment: 4509984 INFO @ Sat, 15 Jan 2022 18:20:54: #1 Redundant rate of treatment: 0.53 INFO @ Sat, 15 Jan 2022 18:20:54: #1 finished! INFO @ Sat, 15 Jan 2022 18:20:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:20:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:20:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:20:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:20:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:20:58: 22000000 INFO @ Sat, 15 Jan 2022 18:21:05: 23000000 INFO @ Sat, 15 Jan 2022 18:21:12: 24000000 INFO @ Sat, 15 Jan 2022 18:21:18: 25000000 INFO @ Sat, 15 Jan 2022 18:21:20: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:21:20: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:21:20: #1 total tags in treatment: 9613504 INFO @ Sat, 15 Jan 2022 18:21:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:21:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:21:20: #1 tags after filtering in treatment: 4509984 INFO @ Sat, 15 Jan 2022 18:21:20: #1 Redundant rate of treatment: 0.53 INFO @ Sat, 15 Jan 2022 18:21:20: #1 finished! INFO @ Sat, 15 Jan 2022 18:21:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:21:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:21:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:21:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:21:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786299/SRX9786299.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling