Job ID = 14519882 SRX = SRX9786298 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 22830898 spots for SRR13362130/SRR13362130.sra Written 22830898 spots for SRR13362130/SRR13362130.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:28:52 22830898 reads; of these: 22830898 (100.00%) were paired; of these: 8973276 (39.30%) aligned concordantly 0 times 10927900 (47.86%) aligned concordantly exactly 1 time 2929722 (12.83%) aligned concordantly >1 times ---- 8973276 pairs aligned concordantly 0 times; of these: 16365 (0.18%) aligned discordantly 1 time ---- 8956911 pairs aligned 0 times concordantly or discordantly; of these: 17913822 mates make up the pairs; of these: 10420557 (58.17%) aligned 0 times 5958445 (33.26%) aligned exactly 1 time 1534820 (8.57%) aligned >1 times 77.18% overall alignment rate Time searching: 00:28:52 Overall time: 00:28:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1844460 / 13867918 = 0.1330 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:53:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:53:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:53:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:53:29: 1000000 INFO @ Sat, 15 Jan 2022 18:53:36: 2000000 INFO @ Sat, 15 Jan 2022 18:53:44: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:53:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:53:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:53:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:53:51: 4000000 INFO @ Sat, 15 Jan 2022 18:53:58: 5000000 INFO @ Sat, 15 Jan 2022 18:53:58: 1000000 INFO @ Sat, 15 Jan 2022 18:54:05: 2000000 INFO @ Sat, 15 Jan 2022 18:54:05: 6000000 INFO @ Sat, 15 Jan 2022 18:54:12: 3000000 INFO @ Sat, 15 Jan 2022 18:54:13: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:54:19: 4000000 INFO @ Sat, 15 Jan 2022 18:54:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:54:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:54:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:54:20: 8000000 INFO @ Sat, 15 Jan 2022 18:54:26: 5000000 INFO @ Sat, 15 Jan 2022 18:54:28: 9000000 INFO @ Sat, 15 Jan 2022 18:54:29: 1000000 INFO @ Sat, 15 Jan 2022 18:54:34: 6000000 INFO @ Sat, 15 Jan 2022 18:54:35: 10000000 INFO @ Sat, 15 Jan 2022 18:54:38: 2000000 INFO @ Sat, 15 Jan 2022 18:54:41: 7000000 INFO @ Sat, 15 Jan 2022 18:54:42: 11000000 INFO @ Sat, 15 Jan 2022 18:54:46: 3000000 INFO @ Sat, 15 Jan 2022 18:54:48: 8000000 INFO @ Sat, 15 Jan 2022 18:54:50: 12000000 INFO @ Sat, 15 Jan 2022 18:54:54: 4000000 INFO @ Sat, 15 Jan 2022 18:54:55: 9000000 INFO @ Sat, 15 Jan 2022 18:54:56: 13000000 INFO @ Sat, 15 Jan 2022 18:55:01: 5000000 INFO @ Sat, 15 Jan 2022 18:55:02: 10000000 INFO @ Sat, 15 Jan 2022 18:55:04: 14000000 INFO @ Sat, 15 Jan 2022 18:55:07: 6000000 INFO @ Sat, 15 Jan 2022 18:55:09: 11000000 INFO @ Sat, 15 Jan 2022 18:55:11: 15000000 INFO @ Sat, 15 Jan 2022 18:55:14: 7000000 INFO @ Sat, 15 Jan 2022 18:55:16: 12000000 INFO @ Sat, 15 Jan 2022 18:55:18: 16000000 INFO @ Sat, 15 Jan 2022 18:55:20: 8000000 INFO @ Sat, 15 Jan 2022 18:55:23: 13000000 INFO @ Sat, 15 Jan 2022 18:55:25: 17000000 INFO @ Sat, 15 Jan 2022 18:55:26: 9000000 INFO @ Sat, 15 Jan 2022 18:55:30: 14000000 INFO @ Sat, 15 Jan 2022 18:55:33: 10000000 INFO @ Sat, 15 Jan 2022 18:55:33: 18000000 INFO @ Sat, 15 Jan 2022 18:55:36: 15000000 INFO @ Sat, 15 Jan 2022 18:55:40: 11000000 INFO @ Sat, 15 Jan 2022 18:55:41: 19000000 INFO @ Sat, 15 Jan 2022 18:55:44: 16000000 INFO @ Sat, 15 Jan 2022 18:55:46: 12000000 INFO @ Sat, 15 Jan 2022 18:55:48: 20000000 INFO @ Sat, 15 Jan 2022 18:55:51: 17000000 INFO @ Sat, 15 Jan 2022 18:55:53: 13000000 INFO @ Sat, 15 Jan 2022 18:55:55: 21000000 INFO @ Sat, 15 Jan 2022 18:55:58: 18000000 INFO @ Sat, 15 Jan 2022 18:55:59: 14000000 INFO @ Sat, 15 Jan 2022 18:56:03: 22000000 INFO @ Sat, 15 Jan 2022 18:56:06: 19000000 INFO @ Sat, 15 Jan 2022 18:56:06: 15000000 INFO @ Sat, 15 Jan 2022 18:56:11: 23000000 INFO @ Sat, 15 Jan 2022 18:56:13: 20000000 INFO @ Sat, 15 Jan 2022 18:56:13: 16000000 INFO @ Sat, 15 Jan 2022 18:56:18: 24000000 INFO @ Sat, 15 Jan 2022 18:56:20: 17000000 INFO @ Sat, 15 Jan 2022 18:56:21: 21000000 INFO @ Sat, 15 Jan 2022 18:56:27: 25000000 INFO @ Sat, 15 Jan 2022 18:56:28: 18000000 INFO @ Sat, 15 Jan 2022 18:56:28: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:56:34: 26000000 INFO @ Sat, 15 Jan 2022 18:56:36: 19000000 INFO @ Sat, 15 Jan 2022 18:56:36: 23000000 INFO @ Sat, 15 Jan 2022 18:56:42: 27000000 INFO @ Sat, 15 Jan 2022 18:56:42: 20000000 INFO @ Sat, 15 Jan 2022 18:56:44: 24000000 INFO @ Sat, 15 Jan 2022 18:56:49: 28000000 INFO @ Sat, 15 Jan 2022 18:56:50: 21000000 INFO @ Sat, 15 Jan 2022 18:56:51: 25000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:56:57: 22000000 INFO @ Sat, 15 Jan 2022 18:56:57: 29000000 INFO @ Sat, 15 Jan 2022 18:56:59: 26000000 INFO @ Sat, 15 Jan 2022 18:57:03: 23000000 INFO @ Sat, 15 Jan 2022 18:57:05: 30000000 INFO @ Sat, 15 Jan 2022 18:57:06: 27000000 INFO @ Sat, 15 Jan 2022 18:57:10: 24000000 INFO @ Sat, 15 Jan 2022 18:57:13: 31000000 INFO @ Sat, 15 Jan 2022 18:57:14: 28000000 INFO @ Sat, 15 Jan 2022 18:57:17: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:57:17: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:57:17: #1 total tags in treatment: 12013219 INFO @ Sat, 15 Jan 2022 18:57:17: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:57:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:57:17: 25000000 INFO @ Sat, 15 Jan 2022 18:57:18: #1 tags after filtering in treatment: 5340439 INFO @ Sat, 15 Jan 2022 18:57:18: #1 Redundant rate of treatment: 0.56 INFO @ Sat, 15 Jan 2022 18:57:18: #1 finished! INFO @ Sat, 15 Jan 2022 18:57:18: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:57:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:57:18: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:57:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:57:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:57:22: 29000000 INFO @ Sat, 15 Jan 2022 18:57:24: 26000000 INFO @ Sat, 15 Jan 2022 18:57:29: 30000000 INFO @ Sat, 15 Jan 2022 18:57:30: 27000000 INFO @ Sat, 15 Jan 2022 18:57:36: 31000000 INFO @ Sat, 15 Jan 2022 18:57:36: 28000000 INFO @ Sat, 15 Jan 2022 18:57:41: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:57:41: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:57:41: #1 total tags in treatment: 12013219 INFO @ Sat, 15 Jan 2022 18:57:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:57:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:57:41: #1 tags after filtering in treatment: 5340439 INFO @ Sat, 15 Jan 2022 18:57:41: #1 Redundant rate of treatment: 0.56 INFO @ Sat, 15 Jan 2022 18:57:41: #1 finished! INFO @ Sat, 15 Jan 2022 18:57:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:57:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:57:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:57:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:57:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:57:44: 29000000 INFO @ Sat, 15 Jan 2022 18:57:51: 30000000 INFO @ Sat, 15 Jan 2022 18:57:57: 31000000 INFO @ Sat, 15 Jan 2022 18:58:01: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:58:01: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:58:01: #1 total tags in treatment: 12013219 INFO @ Sat, 15 Jan 2022 18:58:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:58:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:58:01: #1 tags after filtering in treatment: 5340439 INFO @ Sat, 15 Jan 2022 18:58:01: #1 Redundant rate of treatment: 0.56 INFO @ Sat, 15 Jan 2022 18:58:01: #1 finished! INFO @ Sat, 15 Jan 2022 18:58:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:58:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:58:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:58:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:58:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786298/SRX9786298.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling