Job ID = 14519876 SRX = SRX9786294 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8872768 spots for SRR13362126/SRR13362126.sra Written 8872768 spots for SRR13362126/SRR13362126.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:19 8872768 reads; of these: 8872768 (100.00%) were paired; of these: 1112972 (12.54%) aligned concordantly 0 times 6164399 (69.48%) aligned concordantly exactly 1 time 1595397 (17.98%) aligned concordantly >1 times ---- 1112972 pairs aligned concordantly 0 times; of these: 13593 (1.22%) aligned discordantly 1 time ---- 1099379 pairs aligned 0 times concordantly or discordantly; of these: 2198758 mates make up the pairs; of these: 1398425 (63.60%) aligned 0 times 631034 (28.70%) aligned exactly 1 time 169299 (7.70%) aligned >1 times 92.12% overall alignment rate Time searching: 00:11:19 Overall time: 00:11:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1025328 / 7770608 = 0.1319 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:15:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:15:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:15:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:15:49: 1000000 INFO @ Sat, 15 Jan 2022 18:16:00: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:16:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:16:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:16:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:16:12: 3000000 INFO @ Sat, 15 Jan 2022 18:16:16: 1000000 INFO @ Sat, 15 Jan 2022 18:16:24: 4000000 INFO @ Sat, 15 Jan 2022 18:16:25: 2000000 INFO @ Sat, 15 Jan 2022 18:16:33: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:16:36: 5000000 INFO @ Sat, 15 Jan 2022 18:16:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:16:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:16:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:16:43: 4000000 INFO @ Sat, 15 Jan 2022 18:16:49: 6000000 INFO @ Sat, 15 Jan 2022 18:16:49: 1000000 INFO @ Sat, 15 Jan 2022 18:16:52: 5000000 INFO @ Sat, 15 Jan 2022 18:16:59: 2000000 INFO @ Sat, 15 Jan 2022 18:17:01: 7000000 INFO @ Sat, 15 Jan 2022 18:17:02: 6000000 INFO @ Sat, 15 Jan 2022 18:17:09: 3000000 INFO @ Sat, 15 Jan 2022 18:17:11: 7000000 INFO @ Sat, 15 Jan 2022 18:17:13: 8000000 INFO @ Sat, 15 Jan 2022 18:17:20: 4000000 INFO @ Sat, 15 Jan 2022 18:17:21: 8000000 INFO @ Sat, 15 Jan 2022 18:17:25: 9000000 INFO @ Sat, 15 Jan 2022 18:17:30: 9000000 INFO @ Sat, 15 Jan 2022 18:17:31: 5000000 INFO @ Sat, 15 Jan 2022 18:17:37: 10000000 INFO @ Sat, 15 Jan 2022 18:17:39: 10000000 INFO @ Sat, 15 Jan 2022 18:17:42: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:17:49: 11000000 INFO @ Sat, 15 Jan 2022 18:17:50: 11000000 INFO @ Sat, 15 Jan 2022 18:17:53: 7000000 INFO @ Sat, 15 Jan 2022 18:18:00: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:18:02: 12000000 INFO @ Sat, 15 Jan 2022 18:18:04: 8000000 INFO @ Sat, 15 Jan 2022 18:18:11: 13000000 INFO @ Sat, 15 Jan 2022 18:18:15: 9000000 INFO @ Sat, 15 Jan 2022 18:18:16: 13000000 INFO @ Sat, 15 Jan 2022 18:18:22: 14000000 INFO @ Sat, 15 Jan 2022 18:18:25: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:18:25: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:18:25: #1 total tags in treatment: 6734737 INFO @ Sat, 15 Jan 2022 18:18:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:18:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:18:25: 10000000 INFO @ Sat, 15 Jan 2022 18:18:26: #1 tags after filtering in treatment: 3708284 INFO @ Sat, 15 Jan 2022 18:18:26: #1 Redundant rate of treatment: 0.45 INFO @ Sat, 15 Jan 2022 18:18:26: #1 finished! INFO @ Sat, 15 Jan 2022 18:18:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:18:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:18:26: #2 number of paired peaks: 25 WARNING @ Sat, 15 Jan 2022 18:18:26: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:18:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:18:30: 14000000 INFO @ Sat, 15 Jan 2022 18:18:34: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:18:34: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:18:34: #1 total tags in treatment: 6734737 INFO @ Sat, 15 Jan 2022 18:18:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:18:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:18:34: #1 tags after filtering in treatment: 3708284 INFO @ Sat, 15 Jan 2022 18:18:34: #1 Redundant rate of treatment: 0.45 INFO @ Sat, 15 Jan 2022 18:18:34: #1 finished! INFO @ Sat, 15 Jan 2022 18:18:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:18:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:18:35: #2 number of paired peaks: 25 WARNING @ Sat, 15 Jan 2022 18:18:35: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:18:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:18:36: 11000000 INFO @ Sat, 15 Jan 2022 18:18:46: 12000000 INFO @ Sat, 15 Jan 2022 18:18:55: 13000000 INFO @ Sat, 15 Jan 2022 18:19:05: 14000000 INFO @ Sat, 15 Jan 2022 18:19:08: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:19:08: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:19:08: #1 total tags in treatment: 6734737 INFO @ Sat, 15 Jan 2022 18:19:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:19:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:19:08: #1 tags after filtering in treatment: 3708284 INFO @ Sat, 15 Jan 2022 18:19:08: #1 Redundant rate of treatment: 0.45 INFO @ Sat, 15 Jan 2022 18:19:08: #1 finished! INFO @ Sat, 15 Jan 2022 18:19:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:19:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:19:08: #2 number of paired peaks: 25 WARNING @ Sat, 15 Jan 2022 18:19:08: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:19:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786294/SRX9786294.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling