Job ID = 14519875 SRX = SRX9786293 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 27608169 spots for SRR13362125/SRR13362125.sra Written 27608169 spots for SRR13362125/SRR13362125.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:05 27608169 reads; of these: 27608169 (100.00%) were paired; of these: 11968958 (43.35%) aligned concordantly 0 times 13198812 (47.81%) aligned concordantly exactly 1 time 2440399 (8.84%) aligned concordantly >1 times ---- 11968958 pairs aligned concordantly 0 times; of these: 78255 (0.65%) aligned discordantly 1 time ---- 11890703 pairs aligned 0 times concordantly or discordantly; of these: 23781406 mates make up the pairs; of these: 13347414 (56.13%) aligned 0 times 8793747 (36.98%) aligned exactly 1 time 1640245 (6.90%) aligned >1 times 75.83% overall alignment rate Time searching: 00:23:05 Overall time: 00:23:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1174680 / 15713249 = 0.0748 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:37:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:37:36: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:37:36: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:37:43: 1000000 INFO @ Sat, 15 Jan 2022 18:37:49: 2000000 INFO @ Sat, 15 Jan 2022 18:37:55: 3000000 INFO @ Sat, 15 Jan 2022 18:38:02: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:38:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:38:06: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:38:06: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:38:08: 5000000 INFO @ Sat, 15 Jan 2022 18:38:14: 1000000 INFO @ Sat, 15 Jan 2022 18:38:15: 6000000 INFO @ Sat, 15 Jan 2022 18:38:21: 7000000 INFO @ Sat, 15 Jan 2022 18:38:22: 2000000 INFO @ Sat, 15 Jan 2022 18:38:28: 8000000 INFO @ Sat, 15 Jan 2022 18:38:30: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:38:34: 9000000 INFO @ Sat, 15 Jan 2022 18:38:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:38:36: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:38:36: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:38:38: 4000000 INFO @ Sat, 15 Jan 2022 18:38:41: 10000000 INFO @ Sat, 15 Jan 2022 18:38:43: 1000000 INFO @ Sat, 15 Jan 2022 18:38:45: 5000000 INFO @ Sat, 15 Jan 2022 18:38:48: 11000000 INFO @ Sat, 15 Jan 2022 18:38:50: 2000000 INFO @ Sat, 15 Jan 2022 18:38:53: 6000000 INFO @ Sat, 15 Jan 2022 18:38:55: 12000000 INFO @ Sat, 15 Jan 2022 18:38:57: 3000000 INFO @ Sat, 15 Jan 2022 18:39:01: 7000000 INFO @ Sat, 15 Jan 2022 18:39:02: 13000000 INFO @ Sat, 15 Jan 2022 18:39:03: 4000000 INFO @ Sat, 15 Jan 2022 18:39:08: 14000000 INFO @ Sat, 15 Jan 2022 18:39:09: 8000000 INFO @ Sat, 15 Jan 2022 18:39:10: 5000000 INFO @ Sat, 15 Jan 2022 18:39:15: 15000000 INFO @ Sat, 15 Jan 2022 18:39:16: 9000000 INFO @ Sat, 15 Jan 2022 18:39:17: 6000000 INFO @ Sat, 15 Jan 2022 18:39:22: 16000000 INFO @ Sat, 15 Jan 2022 18:39:24: 7000000 INFO @ Sat, 15 Jan 2022 18:39:24: 10000000 INFO @ Sat, 15 Jan 2022 18:39:29: 17000000 INFO @ Sat, 15 Jan 2022 18:39:31: 8000000 INFO @ Sat, 15 Jan 2022 18:39:32: 11000000 INFO @ Sat, 15 Jan 2022 18:39:36: 18000000 INFO @ Sat, 15 Jan 2022 18:39:37: 9000000 INFO @ Sat, 15 Jan 2022 18:39:39: 12000000 INFO @ Sat, 15 Jan 2022 18:39:43: 19000000 INFO @ Sat, 15 Jan 2022 18:39:45: 10000000 INFO @ Sat, 15 Jan 2022 18:39:46: 13000000 INFO @ Sat, 15 Jan 2022 18:39:50: 20000000 INFO @ Sat, 15 Jan 2022 18:39:51: 11000000 INFO @ Sat, 15 Jan 2022 18:39:53: 14000000 INFO @ Sat, 15 Jan 2022 18:39:57: 21000000 INFO @ Sat, 15 Jan 2022 18:39:58: 12000000 INFO @ Sat, 15 Jan 2022 18:40:01: 15000000 INFO @ Sat, 15 Jan 2022 18:40:03: 22000000 INFO @ Sat, 15 Jan 2022 18:40:05: 13000000 INFO @ Sat, 15 Jan 2022 18:40:08: 16000000 INFO @ Sat, 15 Jan 2022 18:40:10: 23000000 INFO @ Sat, 15 Jan 2022 18:40:12: 14000000 INFO @ Sat, 15 Jan 2022 18:40:14: 17000000 INFO @ Sat, 15 Jan 2022 18:40:17: 24000000 INFO @ Sat, 15 Jan 2022 18:40:19: 15000000 INFO @ Sat, 15 Jan 2022 18:40:21: 18000000 INFO @ Sat, 15 Jan 2022 18:40:24: 25000000 INFO @ Sat, 15 Jan 2022 18:40:26: 16000000 INFO @ Sat, 15 Jan 2022 18:40:27: 19000000 INFO @ Sat, 15 Jan 2022 18:40:30: 26000000 INFO @ Sat, 15 Jan 2022 18:40:33: 17000000 INFO @ Sat, 15 Jan 2022 18:40:33: 20000000 INFO @ Sat, 15 Jan 2022 18:40:37: 27000000 INFO @ Sat, 15 Jan 2022 18:40:39: 18000000 INFO @ Sat, 15 Jan 2022 18:40:40: 21000000 INFO @ Sat, 15 Jan 2022 18:40:43: 28000000 INFO @ Sat, 15 Jan 2022 18:40:45: 19000000 INFO @ Sat, 15 Jan 2022 18:40:46: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:40:49: 29000000 INFO @ Sat, 15 Jan 2022 18:40:52: 20000000 INFO @ Sat, 15 Jan 2022 18:40:52: 23000000 INFO @ Sat, 15 Jan 2022 18:40:56: 30000000 INFO @ Sat, 15 Jan 2022 18:40:58: 21000000 INFO @ Sat, 15 Jan 2022 18:40:58: 24000000 INFO @ Sat, 15 Jan 2022 18:41:03: 31000000 INFO @ Sat, 15 Jan 2022 18:41:05: 25000000 INFO @ Sat, 15 Jan 2022 18:41:05: 22000000 INFO @ Sat, 15 Jan 2022 18:41:09: 32000000 INFO @ Sat, 15 Jan 2022 18:41:11: 26000000 INFO @ Sat, 15 Jan 2022 18:41:12: 23000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:41:17: 33000000 INFO @ Sat, 15 Jan 2022 18:41:17: 27000000 INFO @ Sat, 15 Jan 2022 18:41:18: 24000000 INFO @ Sat, 15 Jan 2022 18:41:23: 28000000 INFO @ Sat, 15 Jan 2022 18:41:24: 34000000 INFO @ Sat, 15 Jan 2022 18:41:25: 25000000 INFO @ Sat, 15 Jan 2022 18:41:30: 29000000 INFO @ Sat, 15 Jan 2022 18:41:31: 26000000 INFO @ Sat, 15 Jan 2022 18:41:31: 35000000 INFO @ Sat, 15 Jan 2022 18:41:36: 30000000 INFO @ Sat, 15 Jan 2022 18:41:36: 27000000 INFO @ Sat, 15 Jan 2022 18:41:38: 36000000 INFO @ Sat, 15 Jan 2022 18:41:42: 31000000 INFO @ Sat, 15 Jan 2022 18:41:43: 28000000 INFO @ Sat, 15 Jan 2022 18:41:45: 37000000 INFO @ Sat, 15 Jan 2022 18:41:49: 32000000 INFO @ Sat, 15 Jan 2022 18:41:49: 29000000 INFO @ Sat, 15 Jan 2022 18:41:51: 38000000 INFO @ Sat, 15 Jan 2022 18:41:55: 30000000 INFO @ Sat, 15 Jan 2022 18:41:55: 33000000 INFO @ Sat, 15 Jan 2022 18:41:57: 39000000 INFO @ Sat, 15 Jan 2022 18:42:01: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:42:01: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:42:01: #1 total tags in treatment: 14464657 INFO @ Sat, 15 Jan 2022 18:42:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:42:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:42:01: #1 tags after filtering in treatment: 7613767 INFO @ Sat, 15 Jan 2022 18:42:01: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 18:42:01: #1 finished! INFO @ Sat, 15 Jan 2022 18:42:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:42:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:42:01: 31000000 INFO @ Sat, 15 Jan 2022 18:42:01: 34000000 INFO @ Sat, 15 Jan 2022 18:42:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:42:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:42:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:42:07: 32000000 INFO @ Sat, 15 Jan 2022 18:42:08: 35000000 INFO @ Sat, 15 Jan 2022 18:42:13: 33000000 INFO @ Sat, 15 Jan 2022 18:42:14: 36000000 INFO @ Sat, 15 Jan 2022 18:42:18: 34000000 INFO @ Sat, 15 Jan 2022 18:42:20: 37000000 INFO @ Sat, 15 Jan 2022 18:42:24: 35000000 INFO @ Sat, 15 Jan 2022 18:42:27: 38000000 INFO @ Sat, 15 Jan 2022 18:42:30: 36000000 INFO @ Sat, 15 Jan 2022 18:42:33: 39000000 INFO @ Sat, 15 Jan 2022 18:42:36: 37000000 INFO @ Sat, 15 Jan 2022 18:42:36: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:42:36: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:42:36: #1 total tags in treatment: 14464657 INFO @ Sat, 15 Jan 2022 18:42:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:42:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:42:37: #1 tags after filtering in treatment: 7613767 INFO @ Sat, 15 Jan 2022 18:42:37: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 18:42:37: #1 finished! INFO @ Sat, 15 Jan 2022 18:42:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:42:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:42:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:42:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:42:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:42:41: 38000000 INFO @ Sat, 15 Jan 2022 18:42:47: 39000000 INFO @ Sat, 15 Jan 2022 18:42:50: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:42:50: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:42:50: #1 total tags in treatment: 14464657 INFO @ Sat, 15 Jan 2022 18:42:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:42:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:42:50: #1 tags after filtering in treatment: 7613767 INFO @ Sat, 15 Jan 2022 18:42:50: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 18:42:50: #1 finished! INFO @ Sat, 15 Jan 2022 18:42:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:42:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:42:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:42:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:42:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786293/SRX9786293.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling