Job ID = 14519873 SRX = SRX9786292 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 37239733 spots for SRR13362124/SRR13362124.sra Written 37239733 spots for SRR13362124/SRR13362124.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:38 37239733 reads; of these: 37239733 (100.00%) were paired; of these: 12563251 (33.74%) aligned concordantly 0 times 21213700 (56.97%) aligned concordantly exactly 1 time 3462782 (9.30%) aligned concordantly >1 times ---- 12563251 pairs aligned concordantly 0 times; of these: 18023 (0.14%) aligned discordantly 1 time ---- 12545228 pairs aligned 0 times concordantly or discordantly; of these: 25090456 mates make up the pairs; of these: 14471454 (57.68%) aligned 0 times 9117994 (36.34%) aligned exactly 1 time 1501008 (5.98%) aligned >1 times 80.57% overall alignment rate Time searching: 00:20:38 Overall time: 00:20:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2494483 / 24691463 = 0.1010 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:36:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:36:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:36:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:36:08: 1000000 INFO @ Sat, 15 Jan 2022 18:36:13: 2000000 INFO @ Sat, 15 Jan 2022 18:36:18: 3000000 INFO @ Sat, 15 Jan 2022 18:36:22: 4000000 INFO @ Sat, 15 Jan 2022 18:36:27: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:36:32: 6000000 INFO @ Sat, 15 Jan 2022 18:36:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:36:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:36:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:36:37: 7000000 INFO @ Sat, 15 Jan 2022 18:36:38: 1000000 INFO @ Sat, 15 Jan 2022 18:36:42: 8000000 INFO @ Sat, 15 Jan 2022 18:36:44: 2000000 INFO @ Sat, 15 Jan 2022 18:36:48: 9000000 INFO @ Sat, 15 Jan 2022 18:36:50: 3000000 INFO @ Sat, 15 Jan 2022 18:36:53: 10000000 INFO @ Sat, 15 Jan 2022 18:36:56: 4000000 INFO @ Sat, 15 Jan 2022 18:36:58: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:37:02: 5000000 INFO @ Sat, 15 Jan 2022 18:37:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:37:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:37:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:37:03: 12000000 INFO @ Sat, 15 Jan 2022 18:37:07: 6000000 INFO @ Sat, 15 Jan 2022 18:37:08: 1000000 INFO @ Sat, 15 Jan 2022 18:37:08: 13000000 INFO @ Sat, 15 Jan 2022 18:37:13: 2000000 INFO @ Sat, 15 Jan 2022 18:37:13: 14000000 INFO @ Sat, 15 Jan 2022 18:37:13: 7000000 INFO @ Sat, 15 Jan 2022 18:37:18: 3000000 INFO @ Sat, 15 Jan 2022 18:37:18: 15000000 INFO @ Sat, 15 Jan 2022 18:37:19: 8000000 INFO @ Sat, 15 Jan 2022 18:37:23: 4000000 INFO @ Sat, 15 Jan 2022 18:37:23: 16000000 INFO @ Sat, 15 Jan 2022 18:37:25: 9000000 INFO @ Sat, 15 Jan 2022 18:37:28: 5000000 INFO @ Sat, 15 Jan 2022 18:37:29: 17000000 INFO @ Sat, 15 Jan 2022 18:37:31: 10000000 INFO @ Sat, 15 Jan 2022 18:37:33: 6000000 INFO @ Sat, 15 Jan 2022 18:37:34: 18000000 INFO @ Sat, 15 Jan 2022 18:37:37: 11000000 INFO @ Sat, 15 Jan 2022 18:37:38: 7000000 INFO @ Sat, 15 Jan 2022 18:37:39: 19000000 INFO @ Sat, 15 Jan 2022 18:37:43: 12000000 INFO @ Sat, 15 Jan 2022 18:37:43: 8000000 INFO @ Sat, 15 Jan 2022 18:37:44: 20000000 INFO @ Sat, 15 Jan 2022 18:37:48: 9000000 INFO @ Sat, 15 Jan 2022 18:37:49: 13000000 INFO @ Sat, 15 Jan 2022 18:37:49: 21000000 INFO @ Sat, 15 Jan 2022 18:37:54: 10000000 INFO @ Sat, 15 Jan 2022 18:37:54: 22000000 INFO @ Sat, 15 Jan 2022 18:37:55: 14000000 INFO @ Sat, 15 Jan 2022 18:37:59: 11000000 INFO @ Sat, 15 Jan 2022 18:37:59: 23000000 INFO @ Sat, 15 Jan 2022 18:38:01: 15000000 INFO @ Sat, 15 Jan 2022 18:38:04: 12000000 INFO @ Sat, 15 Jan 2022 18:38:04: 24000000 INFO @ Sat, 15 Jan 2022 18:38:07: 16000000 INFO @ Sat, 15 Jan 2022 18:38:09: 13000000 INFO @ Sat, 15 Jan 2022 18:38:09: 25000000 INFO @ Sat, 15 Jan 2022 18:38:14: 17000000 INFO @ Sat, 15 Jan 2022 18:38:14: 26000000 INFO @ Sat, 15 Jan 2022 18:38:15: 14000000 INFO @ Sat, 15 Jan 2022 18:38:20: 18000000 INFO @ Sat, 15 Jan 2022 18:38:20: 27000000 INFO @ Sat, 15 Jan 2022 18:38:20: 15000000 INFO @ Sat, 15 Jan 2022 18:38:25: 28000000 INFO @ Sat, 15 Jan 2022 18:38:25: 19000000 INFO @ Sat, 15 Jan 2022 18:38:26: 16000000 INFO @ Sat, 15 Jan 2022 18:38:30: 29000000 INFO @ Sat, 15 Jan 2022 18:38:31: 17000000 INFO @ Sat, 15 Jan 2022 18:38:31: 20000000 INFO @ Sat, 15 Jan 2022 18:38:35: 30000000 INFO @ Sat, 15 Jan 2022 18:38:36: 18000000 INFO @ Sat, 15 Jan 2022 18:38:37: 21000000 INFO @ Sat, 15 Jan 2022 18:38:40: 31000000 INFO @ Sat, 15 Jan 2022 18:38:41: 19000000 INFO @ Sat, 15 Jan 2022 18:38:43: 22000000 INFO @ Sat, 15 Jan 2022 18:38:45: 32000000 INFO @ Sat, 15 Jan 2022 18:38:46: 20000000 INFO @ Sat, 15 Jan 2022 18:38:49: 23000000 INFO @ Sat, 15 Jan 2022 18:38:50: 33000000 INFO @ Sat, 15 Jan 2022 18:38:51: 21000000 INFO @ Sat, 15 Jan 2022 18:38:55: 24000000 INFO @ Sat, 15 Jan 2022 18:38:55: 34000000 INFO @ Sat, 15 Jan 2022 18:38:56: 22000000 INFO @ Sat, 15 Jan 2022 18:39:00: 35000000 INFO @ Sat, 15 Jan 2022 18:39:01: 25000000 INFO @ Sat, 15 Jan 2022 18:39:01: 23000000 INFO @ Sat, 15 Jan 2022 18:39:05: 36000000 INFO @ Sat, 15 Jan 2022 18:39:06: 24000000 INFO @ Sat, 15 Jan 2022 18:39:07: 26000000 INFO @ Sat, 15 Jan 2022 18:39:10: 37000000 INFO @ Sat, 15 Jan 2022 18:39:11: 25000000 INFO @ Sat, 15 Jan 2022 18:39:12: 27000000 INFO @ Sat, 15 Jan 2022 18:39:15: 38000000 INFO @ Sat, 15 Jan 2022 18:39:17: 26000000 INFO @ Sat, 15 Jan 2022 18:39:18: 28000000 INFO @ Sat, 15 Jan 2022 18:39:20: 39000000 INFO @ Sat, 15 Jan 2022 18:39:22: 27000000 INFO @ Sat, 15 Jan 2022 18:39:24: 29000000 INFO @ Sat, 15 Jan 2022 18:39:25: 40000000 INFO @ Sat, 15 Jan 2022 18:39:27: 28000000 INFO @ Sat, 15 Jan 2022 18:39:30: 30000000 INFO @ Sat, 15 Jan 2022 18:39:30: 41000000 INFO @ Sat, 15 Jan 2022 18:39:32: 29000000 INFO @ Sat, 15 Jan 2022 18:39:35: 42000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:39:36: 31000000 INFO @ Sat, 15 Jan 2022 18:39:37: 30000000 INFO @ Sat, 15 Jan 2022 18:39:41: 43000000 INFO @ Sat, 15 Jan 2022 18:39:42: 32000000 INFO @ Sat, 15 Jan 2022 18:39:42: 31000000 INFO @ Sat, 15 Jan 2022 18:39:46: 44000000 INFO @ Sat, 15 Jan 2022 18:39:47: 32000000 INFO @ Sat, 15 Jan 2022 18:39:48: 33000000 INFO @ Sat, 15 Jan 2022 18:39:51: 45000000 INFO @ Sat, 15 Jan 2022 18:39:52: 33000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:39:53: 34000000 INFO @ Sat, 15 Jan 2022 18:39:56: 46000000 INFO @ Sat, 15 Jan 2022 18:39:57: 34000000 INFO @ Sat, 15 Jan 2022 18:39:59: 35000000 INFO @ Sat, 15 Jan 2022 18:40:01: 47000000 INFO @ Sat, 15 Jan 2022 18:40:02: 35000000 INFO @ Sat, 15 Jan 2022 18:40:05: 36000000 INFO @ Sat, 15 Jan 2022 18:40:06: 48000000 INFO @ Sat, 15 Jan 2022 18:40:07: 36000000 INFO @ Sat, 15 Jan 2022 18:40:11: 37000000 INFO @ Sat, 15 Jan 2022 18:40:11: 49000000 INFO @ Sat, 15 Jan 2022 18:40:12: 37000000 INFO @ Sat, 15 Jan 2022 18:40:16: 50000000 INFO @ Sat, 15 Jan 2022 18:40:16: 38000000 INFO @ Sat, 15 Jan 2022 18:40:17: 38000000 INFO @ Sat, 15 Jan 2022 18:40:21: 51000000 INFO @ Sat, 15 Jan 2022 18:40:22: 39000000 INFO @ Sat, 15 Jan 2022 18:40:23: 39000000 INFO @ Sat, 15 Jan 2022 18:40:26: 52000000 INFO @ Sat, 15 Jan 2022 18:40:28: 40000000 INFO @ Sat, 15 Jan 2022 18:40:29: 40000000 INFO @ Sat, 15 Jan 2022 18:40:31: 53000000 INFO @ Sat, 15 Jan 2022 18:40:34: 41000000 INFO @ Sat, 15 Jan 2022 18:40:35: 41000000 INFO @ Sat, 15 Jan 2022 18:40:36: 54000000 INFO @ Sat, 15 Jan 2022 18:40:40: 42000000 INFO @ Sat, 15 Jan 2022 18:40:40: 42000000 INFO @ Sat, 15 Jan 2022 18:40:41: 55000000 INFO @ Sat, 15 Jan 2022 18:40:41: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:40:41: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:40:41: #1 total tags in treatment: 22182044 INFO @ Sat, 15 Jan 2022 18:40:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:40:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:40:42: #1 tags after filtering in treatment: 8299381 INFO @ Sat, 15 Jan 2022 18:40:42: #1 Redundant rate of treatment: 0.63 INFO @ Sat, 15 Jan 2022 18:40:42: #1 finished! INFO @ Sat, 15 Jan 2022 18:40:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:40:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:40:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:40:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:40:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:40:46: 43000000 INFO @ Sat, 15 Jan 2022 18:40:46: 43000000 INFO @ Sat, 15 Jan 2022 18:40:51: 44000000 INFO @ Sat, 15 Jan 2022 18:40:52: 44000000 INFO @ Sat, 15 Jan 2022 18:40:57: 45000000 INFO @ Sat, 15 Jan 2022 18:40:58: 45000000 INFO @ Sat, 15 Jan 2022 18:41:03: 46000000 INFO @ Sat, 15 Jan 2022 18:41:04: 46000000 INFO @ Sat, 15 Jan 2022 18:41:09: 47000000 INFO @ Sat, 15 Jan 2022 18:41:10: 47000000 INFO @ Sat, 15 Jan 2022 18:41:15: 48000000 INFO @ Sat, 15 Jan 2022 18:41:16: 48000000 INFO @ Sat, 15 Jan 2022 18:41:21: 49000000 INFO @ Sat, 15 Jan 2022 18:41:22: 49000000 INFO @ Sat, 15 Jan 2022 18:41:27: 50000000 INFO @ Sat, 15 Jan 2022 18:41:27: 50000000 INFO @ Sat, 15 Jan 2022 18:41:32: 51000000 INFO @ Sat, 15 Jan 2022 18:41:33: 51000000 INFO @ Sat, 15 Jan 2022 18:41:38: 52000000 INFO @ Sat, 15 Jan 2022 18:41:38: 52000000 INFO @ Sat, 15 Jan 2022 18:41:44: 53000000 INFO @ Sat, 15 Jan 2022 18:41:44: 53000000 INFO @ Sat, 15 Jan 2022 18:41:49: 54000000 INFO @ Sat, 15 Jan 2022 18:41:50: 54000000 INFO @ Sat, 15 Jan 2022 18:41:55: 55000000 INFO @ Sat, 15 Jan 2022 18:41:55: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:41:55: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:41:55: #1 total tags in treatment: 22182044 INFO @ Sat, 15 Jan 2022 18:41:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:41:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:41:56: 55000000 INFO @ Sat, 15 Jan 2022 18:41:56: #1 tags after filtering in treatment: 8299381 INFO @ Sat, 15 Jan 2022 18:41:56: #1 Redundant rate of treatment: 0.63 INFO @ Sat, 15 Jan 2022 18:41:56: #1 finished! INFO @ Sat, 15 Jan 2022 18:41:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:41:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:41:56: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:41:56: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:41:56: #1 total tags in treatment: 22182044 INFO @ Sat, 15 Jan 2022 18:41:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:41:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:41:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:41:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:41:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.10_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 18:41:56: #1 tags after filtering in treatment: 8299381 INFO @ Sat, 15 Jan 2022 18:41:56: #1 Redundant rate of treatment: 0.63 INFO @ Sat, 15 Jan 2022 18:41:56: #1 finished! INFO @ Sat, 15 Jan 2022 18:41:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:41:56: #2 looking for paired plus/minus strand peaks... pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:41:57: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:41:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:41:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786292/SRX9786292.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling