Job ID = 14519871 SRX = SRX9786290 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 31823200 spots for SRR13362122/SRR13362122.sra Written 31823200 spots for SRR13362122/SRR13362122.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:40:02 31823200 reads; of these: 31823200 (100.00%) were paired; of these: 10914632 (34.30%) aligned concordantly 0 times 17880824 (56.19%) aligned concordantly exactly 1 time 3027744 (9.51%) aligned concordantly >1 times ---- 10914632 pairs aligned concordantly 0 times; of these: 13445 (0.12%) aligned discordantly 1 time ---- 10901187 pairs aligned 0 times concordantly or discordantly; of these: 21802374 mates make up the pairs; of these: 12473719 (57.21%) aligned 0 times 7976374 (36.58%) aligned exactly 1 time 1352281 (6.20%) aligned >1 times 80.40% overall alignment rate Time searching: 00:40:02 Overall time: 00:40:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2021720 / 20919919 = 0.0966 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:12:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:12:01: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:12:01: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:12:14: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:12:28: 2000000 INFO @ Sat, 15 Jan 2022 19:12:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:12:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:12:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:12:39: 3000000 INFO @ Sat, 15 Jan 2022 19:12:41: 1000000 INFO @ Sat, 15 Jan 2022 19:12:52: 2000000 INFO @ Sat, 15 Jan 2022 19:12:52: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:13:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:13:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:13:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:13:03: 3000000 INFO @ Sat, 15 Jan 2022 19:13:04: 5000000 INFO @ Sat, 15 Jan 2022 19:13:10: 1000000 INFO @ Sat, 15 Jan 2022 19:13:13: 4000000 INFO @ Sat, 15 Jan 2022 19:13:16: 6000000 INFO @ Sat, 15 Jan 2022 19:13:19: 2000000 INFO @ Sat, 15 Jan 2022 19:13:23: 5000000 INFO @ Sat, 15 Jan 2022 19:13:28: 3000000 INFO @ Sat, 15 Jan 2022 19:13:28: 7000000 INFO @ Sat, 15 Jan 2022 19:13:32: 6000000 INFO @ Sat, 15 Jan 2022 19:13:36: 4000000 INFO @ Sat, 15 Jan 2022 19:13:37: 8000000 INFO @ Sat, 15 Jan 2022 19:13:41: 7000000 INFO @ Sat, 15 Jan 2022 19:13:45: 5000000 INFO @ Sat, 15 Jan 2022 19:13:46: 9000000 INFO @ Sat, 15 Jan 2022 19:13:52: 8000000 INFO @ Sat, 15 Jan 2022 19:13:54: 6000000 INFO @ Sat, 15 Jan 2022 19:13:55: 10000000 INFO @ Sat, 15 Jan 2022 19:14:02: 9000000 INFO @ Sat, 15 Jan 2022 19:14:04: 7000000 INFO @ Sat, 15 Jan 2022 19:14:05: 11000000 INFO @ Sat, 15 Jan 2022 19:14:13: 10000000 INFO @ Sat, 15 Jan 2022 19:14:14: 8000000 INFO @ Sat, 15 Jan 2022 19:14:16: 12000000 INFO @ Sat, 15 Jan 2022 19:14:24: 11000000 INFO @ Sat, 15 Jan 2022 19:14:25: 9000000 INFO @ Sat, 15 Jan 2022 19:14:26: 13000000 INFO @ Sat, 15 Jan 2022 19:14:35: 12000000 INFO @ Sat, 15 Jan 2022 19:14:36: 10000000 INFO @ Sat, 15 Jan 2022 19:14:38: 14000000 INFO @ Sat, 15 Jan 2022 19:14:48: 13000000 INFO @ Sat, 15 Jan 2022 19:14:49: 11000000 INFO @ Sat, 15 Jan 2022 19:14:52: 15000000 INFO @ Sat, 15 Jan 2022 19:15:01: 14000000 INFO @ Sat, 15 Jan 2022 19:15:03: 12000000 INFO @ Sat, 15 Jan 2022 19:15:04: 16000000 INFO @ Sat, 15 Jan 2022 19:15:12: 15000000 INFO @ Sat, 15 Jan 2022 19:15:15: 13000000 INFO @ Sat, 15 Jan 2022 19:15:15: 17000000 INFO @ Sat, 15 Jan 2022 19:15:22: 16000000 INFO @ Sat, 15 Jan 2022 19:15:25: 18000000 INFO @ Sat, 15 Jan 2022 19:15:26: 14000000 INFO @ Sat, 15 Jan 2022 19:15:30: 17000000 INFO @ Sat, 15 Jan 2022 19:15:35: 19000000 INFO @ Sat, 15 Jan 2022 19:15:37: 15000000 INFO @ Sat, 15 Jan 2022 19:15:42: 18000000 INFO @ Sat, 15 Jan 2022 19:15:47: 20000000 INFO @ Sat, 15 Jan 2022 19:15:50: 16000000 INFO @ Sat, 15 Jan 2022 19:15:53: 19000000 INFO @ Sat, 15 Jan 2022 19:16:01: 21000000 INFO @ Sat, 15 Jan 2022 19:16:04: 17000000 INFO @ Sat, 15 Jan 2022 19:16:05: 20000000 INFO @ Sat, 15 Jan 2022 19:16:11: 22000000 INFO @ Sat, 15 Jan 2022 19:16:15: 21000000 INFO @ Sat, 15 Jan 2022 19:16:15: 18000000 INFO @ Sat, 15 Jan 2022 19:16:20: 23000000 INFO @ Sat, 15 Jan 2022 19:16:24: 22000000 INFO @ Sat, 15 Jan 2022 19:16:25: 19000000 INFO @ Sat, 15 Jan 2022 19:16:30: 24000000 INFO @ Sat, 15 Jan 2022 19:16:33: 23000000 INFO @ Sat, 15 Jan 2022 19:16:35: 20000000 INFO @ Sat, 15 Jan 2022 19:16:39: 25000000 INFO @ Sat, 15 Jan 2022 19:16:41: 24000000 INFO @ Sat, 15 Jan 2022 19:16:46: 21000000 INFO @ Sat, 15 Jan 2022 19:16:48: 26000000 INFO @ Sat, 15 Jan 2022 19:16:50: 25000000 INFO @ Sat, 15 Jan 2022 19:16:56: 22000000 INFO @ Sat, 15 Jan 2022 19:16:57: 27000000 INFO @ Sat, 15 Jan 2022 19:16:59: 26000000 INFO @ Sat, 15 Jan 2022 19:17:07: 28000000 INFO @ Sat, 15 Jan 2022 19:17:07: 23000000 INFO @ Sat, 15 Jan 2022 19:17:09: 27000000 INFO @ Sat, 15 Jan 2022 19:17:16: 29000000 INFO @ Sat, 15 Jan 2022 19:17:18: 24000000 INFO @ Sat, 15 Jan 2022 19:17:18: 28000000 INFO @ Sat, 15 Jan 2022 19:17:26: 30000000 INFO @ Sat, 15 Jan 2022 19:17:28: 29000000 INFO @ Sat, 15 Jan 2022 19:17:28: 25000000 INFO @ Sat, 15 Jan 2022 19:17:35: 31000000 INFO @ Sat, 15 Jan 2022 19:17:37: 30000000 INFO @ Sat, 15 Jan 2022 19:17:39: 26000000 INFO @ Sat, 15 Jan 2022 19:17:46: 32000000 INFO @ Sat, 15 Jan 2022 19:17:47: 31000000 INFO @ Sat, 15 Jan 2022 19:17:50: 27000000 INFO @ Sat, 15 Jan 2022 19:17:56: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:17:58: 32000000 INFO @ Sat, 15 Jan 2022 19:18:01: 28000000 INFO @ Sat, 15 Jan 2022 19:18:06: 34000000 INFO @ Sat, 15 Jan 2022 19:18:07: 33000000 INFO @ Sat, 15 Jan 2022 19:18:12: 29000000 INFO @ Sat, 15 Jan 2022 19:18:16: 34000000 INFO @ Sat, 15 Jan 2022 19:18:16: 35000000 INFO @ Sat, 15 Jan 2022 19:18:22: 30000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:18:27: 35000000 INFO @ Sat, 15 Jan 2022 19:18:27: 36000000 INFO @ Sat, 15 Jan 2022 19:18:33: 31000000 INFO @ Sat, 15 Jan 2022 19:18:38: 36000000 INFO @ Sat, 15 Jan 2022 19:18:38: 37000000 INFO @ Sat, 15 Jan 2022 19:18:45: 32000000 INFO @ Sat, 15 Jan 2022 19:18:48: 37000000 INFO @ Sat, 15 Jan 2022 19:18:48: 38000000 INFO @ Sat, 15 Jan 2022 19:18:56: 33000000 INFO @ Sat, 15 Jan 2022 19:18:58: 38000000 INFO @ Sat, 15 Jan 2022 19:18:59: 39000000 INFO @ Sat, 15 Jan 2022 19:19:08: 34000000 INFO @ Sat, 15 Jan 2022 19:19:09: 39000000 INFO @ Sat, 15 Jan 2022 19:19:10: 40000000 INFO @ Sat, 15 Jan 2022 19:19:22: 40000000 INFO @ Sat, 15 Jan 2022 19:19:23: 35000000 INFO @ Sat, 15 Jan 2022 19:19:23: 41000000 INFO @ Sat, 15 Jan 2022 19:19:35: 41000000 INFO @ Sat, 15 Jan 2022 19:19:38: 36000000 INFO @ Sat, 15 Jan 2022 19:19:38: 42000000 INFO @ Sat, 15 Jan 2022 19:19:48: 42000000 INFO @ Sat, 15 Jan 2022 19:19:49: 43000000 INFO @ Sat, 15 Jan 2022 19:19:50: 37000000 INFO @ Sat, 15 Jan 2022 19:19:59: 43000000 INFO @ Sat, 15 Jan 2022 19:20:00: 44000000 INFO @ Sat, 15 Jan 2022 19:20:01: 38000000 INFO @ Sat, 15 Jan 2022 19:20:08: 44000000 INFO @ Sat, 15 Jan 2022 19:20:10: 45000000 INFO @ Sat, 15 Jan 2022 19:20:12: 39000000 INFO @ Sat, 15 Jan 2022 19:20:19: 45000000 INFO @ Sat, 15 Jan 2022 19:20:22: 46000000 INFO @ Sat, 15 Jan 2022 19:20:24: 40000000 INFO @ Sat, 15 Jan 2022 19:20:30: 46000000 INFO @ Sat, 15 Jan 2022 19:20:34: 47000000 INFO @ Sat, 15 Jan 2022 19:20:36: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:20:36: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:20:36: #1 total tags in treatment: 18886887 INFO @ Sat, 15 Jan 2022 19:20:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:20:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:20:36: #1 tags after filtering in treatment: 7568404 INFO @ Sat, 15 Jan 2022 19:20:36: #1 Redundant rate of treatment: 0.60 INFO @ Sat, 15 Jan 2022 19:20:36: #1 finished! INFO @ Sat, 15 Jan 2022 19:20:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:20:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:20:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:20:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:20:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:20:39: 41000000 INFO @ Sat, 15 Jan 2022 19:20:44: 47000000 INFO @ Sat, 15 Jan 2022 19:20:46: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:20:46: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:20:46: #1 total tags in treatment: 18886887 INFO @ Sat, 15 Jan 2022 19:20:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:20:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:20:47: #1 tags after filtering in treatment: 7568404 INFO @ Sat, 15 Jan 2022 19:20:47: #1 Redundant rate of treatment: 0.60 INFO @ Sat, 15 Jan 2022 19:20:47: #1 finished! INFO @ Sat, 15 Jan 2022 19:20:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:20:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:20:47: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:20:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:20:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:20:54: 42000000 INFO @ Sat, 15 Jan 2022 19:21:08: 43000000 INFO @ Sat, 15 Jan 2022 19:21:23: 44000000 INFO @ Sat, 15 Jan 2022 19:21:36: 45000000 INFO @ Sat, 15 Jan 2022 19:21:49: 46000000 INFO @ Sat, 15 Jan 2022 19:22:00: 47000000 INFO @ Sat, 15 Jan 2022 19:22:02: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:22:02: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:22:02: #1 total tags in treatment: 18886887 INFO @ Sat, 15 Jan 2022 19:22:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:22:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:22:03: #1 tags after filtering in treatment: 7568404 INFO @ Sat, 15 Jan 2022 19:22:03: #1 Redundant rate of treatment: 0.60 INFO @ Sat, 15 Jan 2022 19:22:03: #1 finished! INFO @ Sat, 15 Jan 2022 19:22:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:22:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:22:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:22:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:22:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786290/SRX9786290.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling