Job ID = 14519845 SRX = SRX9786288 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9113612 spots for SRR13362120/SRR13362120.sra Written 9113612 spots for SRR13362120/SRR13362120.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:15 9113612 reads; of these: 9113612 (100.00%) were paired; of these: 1096153 (12.03%) aligned concordantly 0 times 6803934 (74.66%) aligned concordantly exactly 1 time 1213525 (13.32%) aligned concordantly >1 times ---- 1096153 pairs aligned concordantly 0 times; of these: 9823 (0.90%) aligned discordantly 1 time ---- 1086330 pairs aligned 0 times concordantly or discordantly; of these: 2172660 mates make up the pairs; of these: 1370519 (63.08%) aligned 0 times 669428 (30.81%) aligned exactly 1 time 132713 (6.11%) aligned >1 times 92.48% overall alignment rate Time searching: 00:07:15 Overall time: 00:07:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 710978 / 8026488 = 0.0886 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:05:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:05:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:05:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:05:10: 1000000 INFO @ Sat, 15 Jan 2022 18:05:16: 2000000 INFO @ Sat, 15 Jan 2022 18:05:22: 3000000 INFO @ Sat, 15 Jan 2022 18:05:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:05:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:05:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:05:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:05:34: 5000000 INFO @ Sat, 15 Jan 2022 18:05:40: 1000000 INFO @ Sat, 15 Jan 2022 18:05:40: 6000000 INFO @ Sat, 15 Jan 2022 18:05:47: 2000000 INFO @ Sat, 15 Jan 2022 18:05:47: 7000000 INFO @ Sat, 15 Jan 2022 18:05:54: 3000000 INFO @ Sat, 15 Jan 2022 18:05:54: 8000000 INFO @ Sat, 15 Jan 2022 18:06:01: 4000000 INFO @ Sat, 15 Jan 2022 18:06:01: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:06:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:06:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:06:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:06:08: 5000000 INFO @ Sat, 15 Jan 2022 18:06:08: 10000000 INFO @ Sat, 15 Jan 2022 18:06:13: 1000000 INFO @ Sat, 15 Jan 2022 18:06:16: 6000000 INFO @ Sat, 15 Jan 2022 18:06:16: 11000000 INFO @ Sat, 15 Jan 2022 18:06:22: 2000000 INFO @ Sat, 15 Jan 2022 18:06:24: 7000000 INFO @ Sat, 15 Jan 2022 18:06:24: 12000000 INFO @ Sat, 15 Jan 2022 18:06:31: 3000000 INFO @ Sat, 15 Jan 2022 18:06:32: 8000000 INFO @ Sat, 15 Jan 2022 18:06:32: 13000000 INFO @ Sat, 15 Jan 2022 18:06:40: 9000000 INFO @ Sat, 15 Jan 2022 18:06:40: 14000000 INFO @ Sat, 15 Jan 2022 18:06:40: 4000000 INFO @ Sat, 15 Jan 2022 18:06:47: 15000000 INFO @ Sat, 15 Jan 2022 18:06:47: 10000000 INFO @ Sat, 15 Jan 2022 18:06:49: 5000000 INFO @ Sat, 15 Jan 2022 18:06:51: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:06:51: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:06:51: #1 total tags in treatment: 7306670 INFO @ Sat, 15 Jan 2022 18:06:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:06:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:06:51: #1 tags after filtering in treatment: 4208851 INFO @ Sat, 15 Jan 2022 18:06:51: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 18:06:51: #1 finished! INFO @ Sat, 15 Jan 2022 18:06:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:06:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:06:51: #2 number of paired peaks: 1 WARNING @ Sat, 15 Jan 2022 18:06:51: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:06:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:06:55: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:06:58: 6000000 INFO @ Sat, 15 Jan 2022 18:07:03: 12000000 INFO @ Sat, 15 Jan 2022 18:07:08: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:07:11: 13000000 INFO @ Sat, 15 Jan 2022 18:07:17: 8000000 INFO @ Sat, 15 Jan 2022 18:07:19: 14000000 INFO @ Sat, 15 Jan 2022 18:07:26: 9000000 INFO @ Sat, 15 Jan 2022 18:07:26: 15000000 INFO @ Sat, 15 Jan 2022 18:07:30: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:07:30: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:07:30: #1 total tags in treatment: 7306670 INFO @ Sat, 15 Jan 2022 18:07:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:07:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:07:30: #1 tags after filtering in treatment: 4208851 INFO @ Sat, 15 Jan 2022 18:07:30: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 18:07:30: #1 finished! INFO @ Sat, 15 Jan 2022 18:07:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:07:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:07:30: #2 number of paired peaks: 1 WARNING @ Sat, 15 Jan 2022 18:07:30: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:07:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:07:34: 10000000 INFO @ Sat, 15 Jan 2022 18:07:42: 11000000 INFO @ Sat, 15 Jan 2022 18:07:49: 12000000 INFO @ Sat, 15 Jan 2022 18:07:56: 13000000 INFO @ Sat, 15 Jan 2022 18:08:04: 14000000 INFO @ Sat, 15 Jan 2022 18:08:11: 15000000 INFO @ Sat, 15 Jan 2022 18:08:15: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:08:15: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:08:15: #1 total tags in treatment: 7306670 INFO @ Sat, 15 Jan 2022 18:08:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:08:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:08:15: #1 tags after filtering in treatment: 4208851 INFO @ Sat, 15 Jan 2022 18:08:15: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 18:08:15: #1 finished! INFO @ Sat, 15 Jan 2022 18:08:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:08:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:08:15: #2 number of paired peaks: 1 WARNING @ Sat, 15 Jan 2022 18:08:15: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:08:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786288/SRX9786288.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling