Job ID = 14519844 SRX = SRX9786287 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 22171560 spots for SRR13362151/SRR13362151.sra Written 22171560 spots for SRR13362151/SRR13362151.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:31 22171560 reads; of these: 22171560 (100.00%) were paired; of these: 9932792 (44.80%) aligned concordantly 0 times 11057422 (49.87%) aligned concordantly exactly 1 time 1181346 (5.33%) aligned concordantly >1 times ---- 9932792 pairs aligned concordantly 0 times; of these: 83595 (0.84%) aligned discordantly 1 time ---- 9849197 pairs aligned 0 times concordantly or discordantly; of these: 19698394 mates make up the pairs; of these: 11126330 (56.48%) aligned 0 times 7648360 (38.83%) aligned exactly 1 time 923704 (4.69%) aligned >1 times 74.91% overall alignment rate Time searching: 00:11:31 Overall time: 00:11:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 234578 / 12320901 = 0.0190 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:14:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:14:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:14:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:14:06: 1000000 INFO @ Sat, 15 Jan 2022 18:14:10: 2000000 INFO @ Sat, 15 Jan 2022 18:14:15: 3000000 INFO @ Sat, 15 Jan 2022 18:14:19: 4000000 INFO @ Sat, 15 Jan 2022 18:14:23: 5000000 INFO @ Sat, 15 Jan 2022 18:14:27: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:14:32: 7000000 INFO @ Sat, 15 Jan 2022 18:14:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:14:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:14:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:14:36: 8000000 INFO @ Sat, 15 Jan 2022 18:14:37: 1000000 INFO @ Sat, 15 Jan 2022 18:14:40: 9000000 INFO @ Sat, 15 Jan 2022 18:14:42: 2000000 INFO @ Sat, 15 Jan 2022 18:14:45: 10000000 INFO @ Sat, 15 Jan 2022 18:14:47: 3000000 INFO @ Sat, 15 Jan 2022 18:14:49: 11000000 INFO @ Sat, 15 Jan 2022 18:14:52: 4000000 INFO @ Sat, 15 Jan 2022 18:14:54: 12000000 INFO @ Sat, 15 Jan 2022 18:14:57: 5000000 INFO @ Sat, 15 Jan 2022 18:14:58: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:15:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:15:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:15:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:15:02: 6000000 INFO @ Sat, 15 Jan 2022 18:15:03: 14000000 INFO @ Sat, 15 Jan 2022 18:15:06: 1000000 INFO @ Sat, 15 Jan 2022 18:15:07: 7000000 INFO @ Sat, 15 Jan 2022 18:15:07: 15000000 INFO @ Sat, 15 Jan 2022 18:15:11: 2000000 INFO @ Sat, 15 Jan 2022 18:15:11: 16000000 INFO @ Sat, 15 Jan 2022 18:15:12: 8000000 INFO @ Sat, 15 Jan 2022 18:15:15: 3000000 INFO @ Sat, 15 Jan 2022 18:15:16: 17000000 INFO @ Sat, 15 Jan 2022 18:15:17: 9000000 INFO @ Sat, 15 Jan 2022 18:15:20: 4000000 INFO @ Sat, 15 Jan 2022 18:15:20: 18000000 INFO @ Sat, 15 Jan 2022 18:15:22: 10000000 INFO @ Sat, 15 Jan 2022 18:15:24: 5000000 INFO @ Sat, 15 Jan 2022 18:15:25: 19000000 INFO @ Sat, 15 Jan 2022 18:15:27: 11000000 INFO @ Sat, 15 Jan 2022 18:15:29: 6000000 INFO @ Sat, 15 Jan 2022 18:15:29: 20000000 INFO @ Sat, 15 Jan 2022 18:15:32: 12000000 INFO @ Sat, 15 Jan 2022 18:15:33: 7000000 INFO @ Sat, 15 Jan 2022 18:15:33: 21000000 INFO @ Sat, 15 Jan 2022 18:15:37: 13000000 INFO @ Sat, 15 Jan 2022 18:15:37: 8000000 INFO @ Sat, 15 Jan 2022 18:15:38: 22000000 INFO @ Sat, 15 Jan 2022 18:15:42: 9000000 INFO @ Sat, 15 Jan 2022 18:15:42: 23000000 INFO @ Sat, 15 Jan 2022 18:15:42: 14000000 INFO @ Sat, 15 Jan 2022 18:15:46: 10000000 INFO @ Sat, 15 Jan 2022 18:15:47: 24000000 INFO @ Sat, 15 Jan 2022 18:15:48: 15000000 INFO @ Sat, 15 Jan 2022 18:15:51: 11000000 INFO @ Sat, 15 Jan 2022 18:15:51: 25000000 INFO @ Sat, 15 Jan 2022 18:15:53: 16000000 INFO @ Sat, 15 Jan 2022 18:15:55: 12000000 INFO @ Sat, 15 Jan 2022 18:15:55: 26000000 INFO @ Sat, 15 Jan 2022 18:15:58: 17000000 INFO @ Sat, 15 Jan 2022 18:15:59: 13000000 INFO @ Sat, 15 Jan 2022 18:16:00: 27000000 INFO @ Sat, 15 Jan 2022 18:16:03: 18000000 INFO @ Sat, 15 Jan 2022 18:16:04: 14000000 INFO @ Sat, 15 Jan 2022 18:16:04: 28000000 INFO @ Sat, 15 Jan 2022 18:16:08: 19000000 INFO @ Sat, 15 Jan 2022 18:16:08: 15000000 INFO @ Sat, 15 Jan 2022 18:16:09: 29000000 INFO @ Sat, 15 Jan 2022 18:16:13: 16000000 INFO @ Sat, 15 Jan 2022 18:16:13: 20000000 INFO @ Sat, 15 Jan 2022 18:16:13: 30000000 INFO @ Sat, 15 Jan 2022 18:16:17: 17000000 INFO @ Sat, 15 Jan 2022 18:16:17: 31000000 INFO @ Sat, 15 Jan 2022 18:16:18: 21000000 INFO @ Sat, 15 Jan 2022 18:16:21: 18000000 INFO @ Sat, 15 Jan 2022 18:16:22: 32000000 INFO @ Sat, 15 Jan 2022 18:16:23: 22000000 INFO @ Sat, 15 Jan 2022 18:16:25: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:16:25: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:16:25: #1 total tags in treatment: 12004404 INFO @ Sat, 15 Jan 2022 18:16:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:16:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:16:25: #1 tags after filtering in treatment: 8542090 INFO @ Sat, 15 Jan 2022 18:16:25: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 18:16:25: #1 finished! INFO @ Sat, 15 Jan 2022 18:16:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:16:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:16:26: 19000000 INFO @ Sat, 15 Jan 2022 18:16:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:16:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:16:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:16:28: 23000000 INFO @ Sat, 15 Jan 2022 18:16:30: 20000000 INFO @ Sat, 15 Jan 2022 18:16:33: 24000000 INFO @ Sat, 15 Jan 2022 18:16:35: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:16:39: 25000000 INFO @ Sat, 15 Jan 2022 18:16:39: 22000000 INFO @ Sat, 15 Jan 2022 18:16:43: 23000000 INFO @ Sat, 15 Jan 2022 18:16:44: 26000000 INFO @ Sat, 15 Jan 2022 18:16:48: 24000000 INFO @ Sat, 15 Jan 2022 18:16:49: 27000000 INFO @ Sat, 15 Jan 2022 18:16:52: 25000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:16:54: 28000000 INFO @ Sat, 15 Jan 2022 18:16:56: 26000000 INFO @ Sat, 15 Jan 2022 18:16:59: 29000000 INFO @ Sat, 15 Jan 2022 18:17:01: 27000000 INFO @ Sat, 15 Jan 2022 18:17:04: 30000000 INFO @ Sat, 15 Jan 2022 18:17:05: 28000000 INFO @ Sat, 15 Jan 2022 18:17:09: 31000000 INFO @ Sat, 15 Jan 2022 18:17:09: 29000000 INFO @ Sat, 15 Jan 2022 18:17:14: 30000000 INFO @ Sat, 15 Jan 2022 18:17:14: 32000000 INFO @ Sat, 15 Jan 2022 18:17:18: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:17:18: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:17:18: #1 total tags in treatment: 12004404 INFO @ Sat, 15 Jan 2022 18:17:18: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:17:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:17:18: #1 tags after filtering in treatment: 8542090 INFO @ Sat, 15 Jan 2022 18:17:18: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 18:17:18: #1 finished! INFO @ Sat, 15 Jan 2022 18:17:18: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:17:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:17:18: 31000000 INFO @ Sat, 15 Jan 2022 18:17:18: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:17:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:17:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:17:22: 32000000 INFO @ Sat, 15 Jan 2022 18:17:26: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:17:26: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:17:26: #1 total tags in treatment: 12004404 INFO @ Sat, 15 Jan 2022 18:17:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:17:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:17:26: #1 tags after filtering in treatment: 8542090 INFO @ Sat, 15 Jan 2022 18:17:26: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 18:17:26: #1 finished! INFO @ Sat, 15 Jan 2022 18:17:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:17:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:17:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:17:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:17:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786287/SRX9786287.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling