Job ID = 14519843 SRX = SRX9786286 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16153814 spots for SRR13362150/SRR13362150.sra Written 16153814 spots for SRR13362150/SRR13362150.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:47 16153814 reads; of these: 16153814 (100.00%) were paired; of these: 6984128 (43.24%) aligned concordantly 0 times 8304061 (51.41%) aligned concordantly exactly 1 time 865625 (5.36%) aligned concordantly >1 times ---- 6984128 pairs aligned concordantly 0 times; of these: 34115 (0.49%) aligned discordantly 1 time ---- 6950013 pairs aligned 0 times concordantly or discordantly; of these: 13900026 mates make up the pairs; of these: 7758259 (55.81%) aligned 0 times 5496025 (39.54%) aligned exactly 1 time 645742 (4.65%) aligned >1 times 75.99% overall alignment rate Time searching: 00:14:47 Overall time: 00:14:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 171266 / 9202629 = 0.0186 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:33:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:33:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:33:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:33:47: 1000000 INFO @ Sat, 15 Jan 2022 18:33:54: 2000000 INFO @ Sat, 15 Jan 2022 18:34:02: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:34:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:34:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:34:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:34:09: 4000000 INFO @ Sat, 15 Jan 2022 18:34:16: 1000000 INFO @ Sat, 15 Jan 2022 18:34:17: 5000000 INFO @ Sat, 15 Jan 2022 18:34:23: 2000000 INFO @ Sat, 15 Jan 2022 18:34:26: 6000000 INFO @ Sat, 15 Jan 2022 18:34:30: 3000000 INFO @ Sat, 15 Jan 2022 18:34:34: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:34:37: 4000000 INFO @ Sat, 15 Jan 2022 18:34:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:34:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:34:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:34:41: 8000000 INFO @ Sat, 15 Jan 2022 18:34:43: 5000000 INFO @ Sat, 15 Jan 2022 18:34:47: 1000000 INFO @ Sat, 15 Jan 2022 18:34:49: 9000000 INFO @ Sat, 15 Jan 2022 18:34:50: 6000000 INFO @ Sat, 15 Jan 2022 18:34:55: 2000000 INFO @ Sat, 15 Jan 2022 18:34:56: 10000000 INFO @ Sat, 15 Jan 2022 18:34:57: 7000000 INFO @ Sat, 15 Jan 2022 18:35:02: 3000000 INFO @ Sat, 15 Jan 2022 18:35:04: 8000000 INFO @ Sat, 15 Jan 2022 18:35:04: 11000000 INFO @ Sat, 15 Jan 2022 18:35:10: 4000000 INFO @ Sat, 15 Jan 2022 18:35:11: 9000000 INFO @ Sat, 15 Jan 2022 18:35:12: 12000000 INFO @ Sat, 15 Jan 2022 18:35:18: 5000000 INFO @ Sat, 15 Jan 2022 18:35:18: 10000000 INFO @ Sat, 15 Jan 2022 18:35:20: 13000000 INFO @ Sat, 15 Jan 2022 18:35:25: 6000000 INFO @ Sat, 15 Jan 2022 18:35:26: 11000000 INFO @ Sat, 15 Jan 2022 18:35:27: 14000000 INFO @ Sat, 15 Jan 2022 18:35:33: 12000000 INFO @ Sat, 15 Jan 2022 18:35:33: 7000000 INFO @ Sat, 15 Jan 2022 18:35:35: 15000000 INFO @ Sat, 15 Jan 2022 18:35:40: 13000000 INFO @ Sat, 15 Jan 2022 18:35:41: 8000000 INFO @ Sat, 15 Jan 2022 18:35:43: 16000000 INFO @ Sat, 15 Jan 2022 18:35:48: 14000000 INFO @ Sat, 15 Jan 2022 18:35:49: 9000000 INFO @ Sat, 15 Jan 2022 18:35:51: 17000000 INFO @ Sat, 15 Jan 2022 18:35:55: 15000000 INFO @ Sat, 15 Jan 2022 18:35:57: 10000000 INFO @ Sat, 15 Jan 2022 18:35:59: 18000000 INFO @ Sat, 15 Jan 2022 18:36:02: 16000000 INFO @ Sat, 15 Jan 2022 18:36:05: 11000000 INFO @ Sat, 15 Jan 2022 18:36:07: 19000000 INFO @ Sat, 15 Jan 2022 18:36:10: 17000000 INFO @ Sat, 15 Jan 2022 18:36:12: 12000000 INFO @ Sat, 15 Jan 2022 18:36:15: 20000000 INFO @ Sat, 15 Jan 2022 18:36:17: 18000000 INFO @ Sat, 15 Jan 2022 18:36:20: 13000000 INFO @ Sat, 15 Jan 2022 18:36:23: 21000000 INFO @ Sat, 15 Jan 2022 18:36:25: 19000000 INFO @ Sat, 15 Jan 2022 18:36:28: 14000000 INFO @ Sat, 15 Jan 2022 18:36:31: 22000000 INFO @ Sat, 15 Jan 2022 18:36:32: 20000000 INFO @ Sat, 15 Jan 2022 18:36:35: 15000000 INFO @ Sat, 15 Jan 2022 18:36:39: 21000000 INFO @ Sat, 15 Jan 2022 18:36:40: 23000000 INFO @ Sat, 15 Jan 2022 18:36:42: 16000000 INFO @ Sat, 15 Jan 2022 18:36:47: 22000000 INFO @ Sat, 15 Jan 2022 18:36:48: 24000000 INFO @ Sat, 15 Jan 2022 18:36:49: 17000000 INFO @ Sat, 15 Jan 2022 18:36:50: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:36:50: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:36:50: #1 total tags in treatment: 8998488 INFO @ Sat, 15 Jan 2022 18:36:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:36:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:36:50: #1 tags after filtering in treatment: 6857021 INFO @ Sat, 15 Jan 2022 18:36:50: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 18:36:50: #1 finished! INFO @ Sat, 15 Jan 2022 18:36:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:36:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:36:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:36:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:36:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:36:54: 23000000 INFO @ Sat, 15 Jan 2022 18:36:56: 18000000 INFO @ Sat, 15 Jan 2022 18:37:00: 24000000 INFO @ Sat, 15 Jan 2022 18:37:02: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:37:02: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:37:02: #1 total tags in treatment: 8998488 INFO @ Sat, 15 Jan 2022 18:37:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:37:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:37:02: #1 tags after filtering in treatment: 6857021 INFO @ Sat, 15 Jan 2022 18:37:02: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 18:37:02: #1 finished! INFO @ Sat, 15 Jan 2022 18:37:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:37:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:37:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:37:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:37:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:37:03: 19000000 INFO @ Sat, 15 Jan 2022 18:37:10: 20000000 INFO @ Sat, 15 Jan 2022 18:37:18: 21000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:37:26: 22000000 INFO @ Sat, 15 Jan 2022 18:37:33: 23000000 INFO @ Sat, 15 Jan 2022 18:37:40: 24000000 INFO @ Sat, 15 Jan 2022 18:37:42: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:37:42: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:37:42: #1 total tags in treatment: 8998488 INFO @ Sat, 15 Jan 2022 18:37:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:37:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:37:42: #1 tags after filtering in treatment: 6857021 INFO @ Sat, 15 Jan 2022 18:37:42: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 18:37:42: #1 finished! INFO @ Sat, 15 Jan 2022 18:37:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:37:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:37:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:37:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:37:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786286/SRX9786286.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling