Job ID = 14519842 SRX = SRX9786285 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 17427069 spots for SRR13362149/SRR13362149.sra Written 17427069 spots for SRR13362149/SRR13362149.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:50 17427069 reads; of these: 17427069 (100.00%) were paired; of these: 8054017 (46.22%) aligned concordantly 0 times 8480436 (48.66%) aligned concordantly exactly 1 time 892616 (5.12%) aligned concordantly >1 times ---- 8054017 pairs aligned concordantly 0 times; of these: 153177 (1.90%) aligned discordantly 1 time ---- 7900840 pairs aligned 0 times concordantly or discordantly; of these: 15801680 mates make up the pairs; of these: 8809029 (55.75%) aligned 0 times 6222695 (39.38%) aligned exactly 1 time 769956 (4.87%) aligned >1 times 74.73% overall alignment rate Time searching: 00:14:50 Overall time: 00:14:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 155778 / 9524649 = 0.0164 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:18:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:18:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:18:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:18:24: 1000000 INFO @ Sat, 15 Jan 2022 18:18:30: 2000000 INFO @ Sat, 15 Jan 2022 18:18:37: 3000000 INFO @ Sat, 15 Jan 2022 18:18:44: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:18:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:18:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:18:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:18:50: 5000000 INFO @ Sat, 15 Jan 2022 18:18:53: 1000000 INFO @ Sat, 15 Jan 2022 18:18:57: 6000000 INFO @ Sat, 15 Jan 2022 18:19:01: 2000000 INFO @ Sat, 15 Jan 2022 18:19:04: 7000000 INFO @ Sat, 15 Jan 2022 18:19:07: 3000000 INFO @ Sat, 15 Jan 2022 18:19:11: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:19:15: 4000000 INFO @ Sat, 15 Jan 2022 18:19:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:19:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:19:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:19:18: 9000000 INFO @ Sat, 15 Jan 2022 18:19:22: 5000000 INFO @ Sat, 15 Jan 2022 18:19:24: 1000000 INFO @ Sat, 15 Jan 2022 18:19:25: 10000000 INFO @ Sat, 15 Jan 2022 18:19:29: 6000000 INFO @ Sat, 15 Jan 2022 18:19:31: 2000000 INFO @ Sat, 15 Jan 2022 18:19:31: 11000000 INFO @ Sat, 15 Jan 2022 18:19:36: 7000000 INFO @ Sat, 15 Jan 2022 18:19:38: 3000000 INFO @ Sat, 15 Jan 2022 18:19:38: 12000000 INFO @ Sat, 15 Jan 2022 18:19:43: 8000000 INFO @ Sat, 15 Jan 2022 18:19:45: 13000000 INFO @ Sat, 15 Jan 2022 18:19:45: 4000000 INFO @ Sat, 15 Jan 2022 18:19:50: 9000000 INFO @ Sat, 15 Jan 2022 18:19:52: 14000000 INFO @ Sat, 15 Jan 2022 18:19:53: 5000000 INFO @ Sat, 15 Jan 2022 18:19:57: 10000000 INFO @ Sat, 15 Jan 2022 18:19:59: 15000000 INFO @ Sat, 15 Jan 2022 18:20:00: 6000000 INFO @ Sat, 15 Jan 2022 18:20:04: 11000000 INFO @ Sat, 15 Jan 2022 18:20:06: 16000000 INFO @ Sat, 15 Jan 2022 18:20:07: 7000000 INFO @ Sat, 15 Jan 2022 18:20:11: 12000000 INFO @ Sat, 15 Jan 2022 18:20:13: 17000000 INFO @ Sat, 15 Jan 2022 18:20:14: 8000000 INFO @ Sat, 15 Jan 2022 18:20:18: 13000000 INFO @ Sat, 15 Jan 2022 18:20:20: 18000000 INFO @ Sat, 15 Jan 2022 18:20:22: 9000000 INFO @ Sat, 15 Jan 2022 18:20:25: 14000000 INFO @ Sat, 15 Jan 2022 18:20:27: 19000000 INFO @ Sat, 15 Jan 2022 18:20:29: 10000000 INFO @ Sat, 15 Jan 2022 18:20:32: 15000000 INFO @ Sat, 15 Jan 2022 18:20:34: 20000000 INFO @ Sat, 15 Jan 2022 18:20:36: 11000000 INFO @ Sat, 15 Jan 2022 18:20:39: 16000000 INFO @ Sat, 15 Jan 2022 18:20:41: 21000000 INFO @ Sat, 15 Jan 2022 18:20:43: 12000000 INFO @ Sat, 15 Jan 2022 18:20:46: 17000000 INFO @ Sat, 15 Jan 2022 18:20:48: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:20:50: 13000000 INFO @ Sat, 15 Jan 2022 18:20:53: 18000000 INFO @ Sat, 15 Jan 2022 18:20:55: 23000000 INFO @ Sat, 15 Jan 2022 18:20:57: 14000000 INFO @ Sat, 15 Jan 2022 18:21:00: 19000000 INFO @ Sat, 15 Jan 2022 18:21:02: 24000000 INFO @ Sat, 15 Jan 2022 18:21:04: 15000000 INFO @ Sat, 15 Jan 2022 18:21:06: 20000000 INFO @ Sat, 15 Jan 2022 18:21:08: 25000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:21:11: 16000000 INFO @ Sat, 15 Jan 2022 18:21:13: 21000000 INFO @ Sat, 15 Jan 2022 18:21:14: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:21:14: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:21:14: #1 total tags in treatment: 9217685 INFO @ Sat, 15 Jan 2022 18:21:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:21:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:21:14: #1 tags after filtering in treatment: 6990000 INFO @ Sat, 15 Jan 2022 18:21:14: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 18:21:14: #1 finished! INFO @ Sat, 15 Jan 2022 18:21:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:21:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:21:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:21:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:21:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:21:18: 17000000 INFO @ Sat, 15 Jan 2022 18:21:20: 22000000 INFO @ Sat, 15 Jan 2022 18:21:26: 18000000 INFO @ Sat, 15 Jan 2022 18:21:27: 23000000 INFO @ Sat, 15 Jan 2022 18:21:33: 19000000 INFO @ Sat, 15 Jan 2022 18:21:34: 24000000 INFO @ Sat, 15 Jan 2022 18:21:40: 25000000 INFO @ Sat, 15 Jan 2022 18:21:40: 20000000 INFO @ Sat, 15 Jan 2022 18:21:45: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:21:45: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:21:45: #1 total tags in treatment: 9217685 INFO @ Sat, 15 Jan 2022 18:21:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:21:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:21:45: #1 tags after filtering in treatment: 6990000 INFO @ Sat, 15 Jan 2022 18:21:45: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 18:21:45: #1 finished! INFO @ Sat, 15 Jan 2022 18:21:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:21:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:21:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:21:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:21:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:21:47: 21000000 INFO @ Sat, 15 Jan 2022 18:21:55: 22000000 INFO @ Sat, 15 Jan 2022 18:22:02: 23000000 INFO @ Sat, 15 Jan 2022 18:22:09: 24000000 INFO @ Sat, 15 Jan 2022 18:22:16: 25000000 INFO @ Sat, 15 Jan 2022 18:22:21: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:22:21: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:22:21: #1 total tags in treatment: 9217685 INFO @ Sat, 15 Jan 2022 18:22:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:22:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:22:21: #1 tags after filtering in treatment: 6990000 INFO @ Sat, 15 Jan 2022 18:22:21: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 18:22:21: #1 finished! INFO @ Sat, 15 Jan 2022 18:22:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:22:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:22:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:22:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:22:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786285/SRX9786285.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling