Job ID = 14519840 SRX = SRX9786284 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11858318 spots for SRR13362148/SRR13362148.sra Written 11858318 spots for SRR13362148/SRR13362148.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:11 11858318 reads; of these: 11858318 (100.00%) were paired; of these: 6160765 (51.95%) aligned concordantly 0 times 4482419 (37.80%) aligned concordantly exactly 1 time 1215134 (10.25%) aligned concordantly >1 times ---- 6160765 pairs aligned concordantly 0 times; of these: 68191 (1.11%) aligned discordantly 1 time ---- 6092574 pairs aligned 0 times concordantly or discordantly; of these: 12185148 mates make up the pairs; of these: 7676346 (63.00%) aligned 0 times 3496166 (28.69%) aligned exactly 1 time 1012636 (8.31%) aligned >1 times 67.63% overall alignment rate Time searching: 00:09:11 Overall time: 00:09:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 723752 / 5764482 = 0.1256 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:06:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:06:06: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:06:06: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:06:13: 1000000 INFO @ Sat, 15 Jan 2022 18:06:19: 2000000 INFO @ Sat, 15 Jan 2022 18:06:25: 3000000 INFO @ Sat, 15 Jan 2022 18:06:31: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:06:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:06:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:06:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:06:38: 5000000 INFO @ Sat, 15 Jan 2022 18:06:42: 1000000 INFO @ Sat, 15 Jan 2022 18:06:45: 6000000 INFO @ Sat, 15 Jan 2022 18:06:49: 2000000 INFO @ Sat, 15 Jan 2022 18:06:53: 7000000 INFO @ Sat, 15 Jan 2022 18:06:55: 3000000 INFO @ Sat, 15 Jan 2022 18:07:00: 8000000 INFO @ Sat, 15 Jan 2022 18:07:02: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:07:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:07:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:07:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:07:08: 9000000 INFO @ Sat, 15 Jan 2022 18:07:09: 5000000 INFO @ Sat, 15 Jan 2022 18:07:13: 1000000 INFO @ Sat, 15 Jan 2022 18:07:16: 10000000 INFO @ Sat, 15 Jan 2022 18:07:16: 6000000 INFO @ Sat, 15 Jan 2022 18:07:20: 2000000 INFO @ Sat, 15 Jan 2022 18:07:23: 7000000 INFO @ Sat, 15 Jan 2022 18:07:23: 11000000 INFO @ Sat, 15 Jan 2022 18:07:28: 3000000 INFO @ Sat, 15 Jan 2022 18:07:30: 8000000 INFO @ Sat, 15 Jan 2022 18:07:31: 12000000 INFO @ Sat, 15 Jan 2022 18:07:35: 4000000 INFO @ Sat, 15 Jan 2022 18:07:37: 9000000 INFO @ Sat, 15 Jan 2022 18:07:39: 13000000 INFO @ Sat, 15 Jan 2022 18:07:43: 5000000 INFO @ Sat, 15 Jan 2022 18:07:44: 10000000 INFO @ Sat, 15 Jan 2022 18:07:47: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:07:51: 6000000 INFO @ Sat, 15 Jan 2022 18:07:51: 11000000 INFO @ Sat, 15 Jan 2022 18:07:52: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:07:52: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:07:52: #1 total tags in treatment: 4975337 INFO @ Sat, 15 Jan 2022 18:07:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:07:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:07:52: #1 tags after filtering in treatment: 2507107 INFO @ Sat, 15 Jan 2022 18:07:52: #1 Redundant rate of treatment: 0.50 INFO @ Sat, 15 Jan 2022 18:07:52: #1 finished! INFO @ Sat, 15 Jan 2022 18:07:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:07:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:07:52: #2 number of paired peaks: 65 WARNING @ Sat, 15 Jan 2022 18:07:52: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:07:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:07:58: 12000000 INFO @ Sat, 15 Jan 2022 18:07:58: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:08:04: 13000000 INFO @ Sat, 15 Jan 2022 18:08:06: 8000000 INFO @ Sat, 15 Jan 2022 18:08:11: 14000000 INFO @ Sat, 15 Jan 2022 18:08:13: 9000000 INFO @ Sat, 15 Jan 2022 18:08:15: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:08:15: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:08:15: #1 total tags in treatment: 4975337 INFO @ Sat, 15 Jan 2022 18:08:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:08:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:08:16: #1 tags after filtering in treatment: 2507107 INFO @ Sat, 15 Jan 2022 18:08:16: #1 Redundant rate of treatment: 0.50 INFO @ Sat, 15 Jan 2022 18:08:16: #1 finished! INFO @ Sat, 15 Jan 2022 18:08:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:08:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:08:16: #2 number of paired peaks: 65 WARNING @ Sat, 15 Jan 2022 18:08:16: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:08:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:08:20: 10000000 INFO @ Sat, 15 Jan 2022 18:08:27: 11000000 INFO @ Sat, 15 Jan 2022 18:08:34: 12000000 INFO @ Sat, 15 Jan 2022 18:08:41: 13000000 INFO @ Sat, 15 Jan 2022 18:08:48: 14000000 INFO @ Sat, 15 Jan 2022 18:08:51: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:08:51: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:08:51: #1 total tags in treatment: 4975337 INFO @ Sat, 15 Jan 2022 18:08:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:08:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:08:51: #1 tags after filtering in treatment: 2507107 INFO @ Sat, 15 Jan 2022 18:08:51: #1 Redundant rate of treatment: 0.50 INFO @ Sat, 15 Jan 2022 18:08:51: #1 finished! INFO @ Sat, 15 Jan 2022 18:08:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:08:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:08:52: #2 number of paired peaks: 65 WARNING @ Sat, 15 Jan 2022 18:08:52: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:08:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786284/SRX9786284.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling