Job ID = 14519839 SRX = SRX9786283 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 14592454 spots for SRR13362147/SRR13362147.sra Written 14592454 spots for SRR13362147/SRR13362147.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:20 14592454 reads; of these: 14592454 (100.00%) were paired; of these: 8342138 (57.17%) aligned concordantly 0 times 5333558 (36.55%) aligned concordantly exactly 1 time 916758 (6.28%) aligned concordantly >1 times ---- 8342138 pairs aligned concordantly 0 times; of these: 189226 (2.27%) aligned discordantly 1 time ---- 8152912 pairs aligned 0 times concordantly or discordantly; of these: 16305824 mates make up the pairs; of these: 10327408 (63.34%) aligned 0 times 4957938 (30.41%) aligned exactly 1 time 1020478 (6.26%) aligned >1 times 64.61% overall alignment rate Time searching: 00:08:20 Overall time: 00:08:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 474407 / 6436979 = 0.0737 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:05:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:05:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:05:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:06:01: 1000000 INFO @ Sat, 15 Jan 2022 18:06:06: 2000000 INFO @ Sat, 15 Jan 2022 18:06:12: 3000000 INFO @ Sat, 15 Jan 2022 18:06:17: 4000000 INFO @ Sat, 15 Jan 2022 18:06:22: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:06:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:06:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:06:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:06:27: 6000000 INFO @ Sat, 15 Jan 2022 18:06:32: 1000000 INFO @ Sat, 15 Jan 2022 18:06:32: 7000000 INFO @ Sat, 15 Jan 2022 18:06:37: 2000000 INFO @ Sat, 15 Jan 2022 18:06:38: 8000000 INFO @ Sat, 15 Jan 2022 18:06:43: 3000000 INFO @ Sat, 15 Jan 2022 18:06:43: 9000000 INFO @ Sat, 15 Jan 2022 18:06:48: 10000000 INFO @ Sat, 15 Jan 2022 18:06:48: 4000000 INFO @ Sat, 15 Jan 2022 18:06:53: 11000000 INFO @ Sat, 15 Jan 2022 18:06:53: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:06:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:06:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:06:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:06:59: 6000000 INFO @ Sat, 15 Jan 2022 18:06:59: 12000000 INFO @ Sat, 15 Jan 2022 18:07:01: 1000000 INFO @ Sat, 15 Jan 2022 18:07:04: 7000000 INFO @ Sat, 15 Jan 2022 18:07:04: 13000000 INFO @ Sat, 15 Jan 2022 18:07:07: 2000000 INFO @ Sat, 15 Jan 2022 18:07:09: 8000000 INFO @ Sat, 15 Jan 2022 18:07:10: 14000000 INFO @ Sat, 15 Jan 2022 18:07:12: 3000000 INFO @ Sat, 15 Jan 2022 18:07:14: 9000000 INFO @ Sat, 15 Jan 2022 18:07:15: 15000000 INFO @ Sat, 15 Jan 2022 18:07:17: 4000000 INFO @ Sat, 15 Jan 2022 18:07:19: 10000000 INFO @ Sat, 15 Jan 2022 18:07:20: 16000000 INFO @ Sat, 15 Jan 2022 18:07:22: 5000000 INFO @ Sat, 15 Jan 2022 18:07:24: 11000000 INFO @ Sat, 15 Jan 2022 18:07:25: 17000000 INFO @ Sat, 15 Jan 2022 18:07:27: 6000000 INFO @ Sat, 15 Jan 2022 18:07:30: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:07:30: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:07:30: #1 total tags in treatment: 5780781 INFO @ Sat, 15 Jan 2022 18:07:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:07:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:07:30: 12000000 INFO @ Sat, 15 Jan 2022 18:07:30: #1 tags after filtering in treatment: 4156602 INFO @ Sat, 15 Jan 2022 18:07:30: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 18:07:30: #1 finished! INFO @ Sat, 15 Jan 2022 18:07:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:07:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:07:30: #2 number of paired peaks: 29 WARNING @ Sat, 15 Jan 2022 18:07:30: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:07:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:07:32: 7000000 INFO @ Sat, 15 Jan 2022 18:07:35: 13000000 INFO @ Sat, 15 Jan 2022 18:07:37: 8000000 INFO @ Sat, 15 Jan 2022 18:07:41: 14000000 INFO @ Sat, 15 Jan 2022 18:07:42: 9000000 INFO @ Sat, 15 Jan 2022 18:07:46: 15000000 INFO @ Sat, 15 Jan 2022 18:07:47: 10000000 INFO @ Sat, 15 Jan 2022 18:07:51: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:07:53: 11000000 INFO @ Sat, 15 Jan 2022 18:07:56: 17000000 INFO @ Sat, 15 Jan 2022 18:07:58: 12000000 INFO @ Sat, 15 Jan 2022 18:08:01: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:08:01: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:08:01: #1 total tags in treatment: 5780781 INFO @ Sat, 15 Jan 2022 18:08:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:08:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:08:01: #1 tags after filtering in treatment: 4156602 INFO @ Sat, 15 Jan 2022 18:08:01: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 18:08:01: #1 finished! INFO @ Sat, 15 Jan 2022 18:08:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:08:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:08:01: #2 number of paired peaks: 29 WARNING @ Sat, 15 Jan 2022 18:08:01: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:08:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:08:03: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:08:08: 14000000 INFO @ Sat, 15 Jan 2022 18:08:13: 15000000 INFO @ Sat, 15 Jan 2022 18:08:18: 16000000 INFO @ Sat, 15 Jan 2022 18:08:22: 17000000 INFO @ Sat, 15 Jan 2022 18:08:26: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:08:26: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:08:26: #1 total tags in treatment: 5780781 INFO @ Sat, 15 Jan 2022 18:08:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:08:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:08:26: #1 tags after filtering in treatment: 4156602 INFO @ Sat, 15 Jan 2022 18:08:26: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 18:08:26: #1 finished! INFO @ Sat, 15 Jan 2022 18:08:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:08:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:08:27: #2 number of paired peaks: 29 WARNING @ Sat, 15 Jan 2022 18:08:27: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:08:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786283/SRX9786283.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling