Job ID = 14519838 SRX = SRX9786282 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15229663 spots for SRR13362146/SRR13362146.sra Written 15229663 spots for SRR13362146/SRR13362146.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:44 15229663 reads; of these: 15229663 (100.00%) were paired; of these: 8487253 (55.73%) aligned concordantly 0 times 5767833 (37.87%) aligned concordantly exactly 1 time 974577 (6.40%) aligned concordantly >1 times ---- 8487253 pairs aligned concordantly 0 times; of these: 131561 (1.55%) aligned discordantly 1 time ---- 8355692 pairs aligned 0 times concordantly or discordantly; of these: 16711384 mates make up the pairs; of these: 11448952 (68.51%) aligned 0 times 4387288 (26.25%) aligned exactly 1 time 875144 (5.24%) aligned >1 times 62.41% overall alignment rate Time searching: 00:11:44 Overall time: 00:11:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 676154 / 6871769 = 0.0984 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:11:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:11:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:11:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:11:14: 1000000 INFO @ Sat, 15 Jan 2022 18:11:19: 2000000 INFO @ Sat, 15 Jan 2022 18:11:24: 3000000 INFO @ Sat, 15 Jan 2022 18:11:29: 4000000 INFO @ Sat, 15 Jan 2022 18:11:34: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:11:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:11:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:11:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:11:40: 6000000 INFO @ Sat, 15 Jan 2022 18:11:44: 1000000 INFO @ Sat, 15 Jan 2022 18:11:46: 7000000 INFO @ Sat, 15 Jan 2022 18:11:50: 2000000 INFO @ Sat, 15 Jan 2022 18:11:52: 8000000 INFO @ Sat, 15 Jan 2022 18:11:56: 3000000 INFO @ Sat, 15 Jan 2022 18:11:58: 9000000 INFO @ Sat, 15 Jan 2022 18:12:02: 4000000 INFO @ Sat, 15 Jan 2022 18:12:03: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:12:07: 5000000 INFO @ Sat, 15 Jan 2022 18:12:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:12:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:12:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:12:09: 11000000 INFO @ Sat, 15 Jan 2022 18:12:14: 6000000 INFO @ Sat, 15 Jan 2022 18:12:15: 1000000 INFO @ Sat, 15 Jan 2022 18:12:15: 12000000 INFO @ Sat, 15 Jan 2022 18:12:20: 7000000 INFO @ Sat, 15 Jan 2022 18:12:21: 2000000 INFO @ Sat, 15 Jan 2022 18:12:21: 13000000 INFO @ Sat, 15 Jan 2022 18:12:26: 8000000 INFO @ Sat, 15 Jan 2022 18:12:27: 3000000 INFO @ Sat, 15 Jan 2022 18:12:28: 14000000 INFO @ Sat, 15 Jan 2022 18:12:32: 9000000 INFO @ Sat, 15 Jan 2022 18:12:34: 4000000 INFO @ Sat, 15 Jan 2022 18:12:34: 15000000 INFO @ Sat, 15 Jan 2022 18:12:38: 10000000 INFO @ Sat, 15 Jan 2022 18:12:40: 5000000 INFO @ Sat, 15 Jan 2022 18:12:40: 16000000 INFO @ Sat, 15 Jan 2022 18:12:44: 11000000 INFO @ Sat, 15 Jan 2022 18:12:46: 6000000 INFO @ Sat, 15 Jan 2022 18:12:46: 17000000 INFO @ Sat, 15 Jan 2022 18:12:50: 12000000 INFO @ Sat, 15 Jan 2022 18:12:51: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:12:51: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:12:51: #1 total tags in treatment: 6070445 INFO @ Sat, 15 Jan 2022 18:12:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:12:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:12:51: #1 tags after filtering in treatment: 4665228 INFO @ Sat, 15 Jan 2022 18:12:51: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 18:12:51: #1 finished! INFO @ Sat, 15 Jan 2022 18:12:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:12:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:12:51: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 18:12:51: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:12:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:12:52: 7000000 INFO @ Sat, 15 Jan 2022 18:12:56: 13000000 INFO @ Sat, 15 Jan 2022 18:12:58: 8000000 INFO @ Sat, 15 Jan 2022 18:13:02: 14000000 INFO @ Sat, 15 Jan 2022 18:13:04: 9000000 INFO @ Sat, 15 Jan 2022 18:13:08: 15000000 INFO @ Sat, 15 Jan 2022 18:13:10: 10000000 INFO @ Sat, 15 Jan 2022 18:13:14: 16000000 INFO @ Sat, 15 Jan 2022 18:13:15: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:13:20: 17000000 INFO @ Sat, 15 Jan 2022 18:13:21: 12000000 INFO @ Sat, 15 Jan 2022 18:13:23: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:13:23: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:13:23: #1 total tags in treatment: 6070445 INFO @ Sat, 15 Jan 2022 18:13:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:13:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:13:23: #1 tags after filtering in treatment: 4665228 INFO @ Sat, 15 Jan 2022 18:13:23: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 18:13:23: #1 finished! INFO @ Sat, 15 Jan 2022 18:13:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:13:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:13:24: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 18:13:24: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:13:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:13:27: 13000000 INFO @ Sat, 15 Jan 2022 18:13:32: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:13:38: 15000000 INFO @ Sat, 15 Jan 2022 18:13:43: 16000000 INFO @ Sat, 15 Jan 2022 18:13:48: 17000000 INFO @ Sat, 15 Jan 2022 18:13:51: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:13:51: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:13:51: #1 total tags in treatment: 6070445 INFO @ Sat, 15 Jan 2022 18:13:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:13:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:13:51: #1 tags after filtering in treatment: 4665228 INFO @ Sat, 15 Jan 2022 18:13:51: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 18:13:51: #1 finished! INFO @ Sat, 15 Jan 2022 18:13:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:13:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:13:52: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 18:13:52: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:13:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786282/SRX9786282.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling