Job ID = 14519818 SRX = SRX9786278 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 30939802 spots for SRR13362142/SRR13362142.sra Written 30939802 spots for SRR13362142/SRR13362142.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:14 30939802 reads; of these: 30939802 (100.00%) were paired; of these: 13029462 (42.11%) aligned concordantly 0 times 16190039 (52.33%) aligned concordantly exactly 1 time 1720301 (5.56%) aligned concordantly >1 times ---- 13029462 pairs aligned concordantly 0 times; of these: 224732 (1.72%) aligned discordantly 1 time ---- 12804730 pairs aligned 0 times concordantly or discordantly; of these: 25609460 mates make up the pairs; of these: 14627519 (57.12%) aligned 0 times 9788823 (38.22%) aligned exactly 1 time 1193118 (4.66%) aligned >1 times 76.36% overall alignment rate Time searching: 00:24:14 Overall time: 00:24:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 361793 / 18132815 = 0.0200 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:32:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:32:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:32:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:32:53: 1000000 INFO @ Sat, 15 Jan 2022 18:33:01: 2000000 INFO @ Sat, 15 Jan 2022 18:33:09: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:33:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:33:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:33:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:33:18: 4000000 INFO @ Sat, 15 Jan 2022 18:33:22: 1000000 INFO @ Sat, 15 Jan 2022 18:33:26: 5000000 INFO @ Sat, 15 Jan 2022 18:33:29: 2000000 INFO @ Sat, 15 Jan 2022 18:33:33: 6000000 INFO @ Sat, 15 Jan 2022 18:33:36: 3000000 INFO @ Sat, 15 Jan 2022 18:33:40: 7000000 INFO @ Sat, 15 Jan 2022 18:33:42: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:33:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:33:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:33:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:33:47: 8000000 INFO @ Sat, 15 Jan 2022 18:33:48: 5000000 INFO @ Sat, 15 Jan 2022 18:33:52: 1000000 INFO @ Sat, 15 Jan 2022 18:33:54: 6000000 INFO @ Sat, 15 Jan 2022 18:33:55: 9000000 INFO @ Sat, 15 Jan 2022 18:34:00: 2000000 INFO @ Sat, 15 Jan 2022 18:34:01: 7000000 INFO @ Sat, 15 Jan 2022 18:34:02: 10000000 INFO @ Sat, 15 Jan 2022 18:34:07: 8000000 INFO @ Sat, 15 Jan 2022 18:34:08: 3000000 INFO @ Sat, 15 Jan 2022 18:34:10: 11000000 INFO @ Sat, 15 Jan 2022 18:34:13: 9000000 INFO @ Sat, 15 Jan 2022 18:34:15: 4000000 INFO @ Sat, 15 Jan 2022 18:34:18: 12000000 INFO @ Sat, 15 Jan 2022 18:34:19: 10000000 INFO @ Sat, 15 Jan 2022 18:34:23: 5000000 INFO @ Sat, 15 Jan 2022 18:34:25: 13000000 INFO @ Sat, 15 Jan 2022 18:34:26: 11000000 INFO @ Sat, 15 Jan 2022 18:34:31: 6000000 INFO @ Sat, 15 Jan 2022 18:34:32: 12000000 INFO @ Sat, 15 Jan 2022 18:34:33: 14000000 INFO @ Sat, 15 Jan 2022 18:34:38: 13000000 INFO @ Sat, 15 Jan 2022 18:34:39: 7000000 INFO @ Sat, 15 Jan 2022 18:34:41: 15000000 INFO @ Sat, 15 Jan 2022 18:34:45: 14000000 INFO @ Sat, 15 Jan 2022 18:34:47: 8000000 INFO @ Sat, 15 Jan 2022 18:34:48: 16000000 INFO @ Sat, 15 Jan 2022 18:34:51: 15000000 INFO @ Sat, 15 Jan 2022 18:34:54: 9000000 INFO @ Sat, 15 Jan 2022 18:34:56: 17000000 INFO @ Sat, 15 Jan 2022 18:34:57: 16000000 INFO @ Sat, 15 Jan 2022 18:35:02: 10000000 INFO @ Sat, 15 Jan 2022 18:35:04: 17000000 INFO @ Sat, 15 Jan 2022 18:35:04: 18000000 INFO @ Sat, 15 Jan 2022 18:35:10: 11000000 INFO @ Sat, 15 Jan 2022 18:35:10: 18000000 INFO @ Sat, 15 Jan 2022 18:35:12: 19000000 INFO @ Sat, 15 Jan 2022 18:35:16: 19000000 INFO @ Sat, 15 Jan 2022 18:35:18: 12000000 INFO @ Sat, 15 Jan 2022 18:35:19: 20000000 INFO @ Sat, 15 Jan 2022 18:35:23: 20000000 INFO @ Sat, 15 Jan 2022 18:35:25: 13000000 INFO @ Sat, 15 Jan 2022 18:35:27: 21000000 INFO @ Sat, 15 Jan 2022 18:35:29: 21000000 INFO @ Sat, 15 Jan 2022 18:35:33: 14000000 INFO @ Sat, 15 Jan 2022 18:35:35: 22000000 INFO @ Sat, 15 Jan 2022 18:35:35: 22000000 INFO @ Sat, 15 Jan 2022 18:35:41: 15000000 INFO @ Sat, 15 Jan 2022 18:35:42: 23000000 INFO @ Sat, 15 Jan 2022 18:35:43: 23000000 INFO @ Sat, 15 Jan 2022 18:35:48: 16000000 INFO @ Sat, 15 Jan 2022 18:35:49: 24000000 INFO @ Sat, 15 Jan 2022 18:35:50: 24000000 INFO @ Sat, 15 Jan 2022 18:35:55: 25000000 INFO @ Sat, 15 Jan 2022 18:35:55: 17000000 INFO @ Sat, 15 Jan 2022 18:35:58: 25000000 INFO @ Sat, 15 Jan 2022 18:36:01: 26000000 INFO @ Sat, 15 Jan 2022 18:36:03: 18000000 INFO @ Sat, 15 Jan 2022 18:36:05: 26000000 INFO @ Sat, 15 Jan 2022 18:36:08: 27000000 INFO @ Sat, 15 Jan 2022 18:36:11: 19000000 INFO @ Sat, 15 Jan 2022 18:36:13: 27000000 INFO @ Sat, 15 Jan 2022 18:36:14: 28000000 INFO @ Sat, 15 Jan 2022 18:36:18: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:36:20: 28000000 INFO @ Sat, 15 Jan 2022 18:36:21: 29000000 INFO @ Sat, 15 Jan 2022 18:36:25: 21000000 INFO @ Sat, 15 Jan 2022 18:36:27: 30000000 INFO @ Sat, 15 Jan 2022 18:36:28: 29000000 INFO @ Sat, 15 Jan 2022 18:36:33: 22000000 INFO @ Sat, 15 Jan 2022 18:36:34: 31000000 INFO @ Sat, 15 Jan 2022 18:36:36: 30000000 INFO @ Sat, 15 Jan 2022 18:36:41: 23000000 INFO @ Sat, 15 Jan 2022 18:36:41: 32000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:36:44: 31000000 INFO @ Sat, 15 Jan 2022 18:36:48: 24000000 INFO @ Sat, 15 Jan 2022 18:36:49: 33000000 INFO @ Sat, 15 Jan 2022 18:36:52: 32000000 INFO @ Sat, 15 Jan 2022 18:36:56: 34000000 INFO @ Sat, 15 Jan 2022 18:36:56: 25000000 INFO @ Sat, 15 Jan 2022 18:36:59: 33000000 INFO @ Sat, 15 Jan 2022 18:37:02: 35000000 INFO @ Sat, 15 Jan 2022 18:37:04: 26000000 INFO @ Sat, 15 Jan 2022 18:37:07: 34000000 INFO @ Sat, 15 Jan 2022 18:37:09: 36000000 INFO @ Sat, 15 Jan 2022 18:37:11: 27000000 INFO @ Sat, 15 Jan 2022 18:37:14: 35000000 INFO @ Sat, 15 Jan 2022 18:37:17: 37000000 INFO @ Sat, 15 Jan 2022 18:37:19: 28000000 INFO @ Sat, 15 Jan 2022 18:37:21: 36000000 INFO @ Sat, 15 Jan 2022 18:37:24: 38000000 INFO @ Sat, 15 Jan 2022 18:37:26: 29000000 INFO @ Sat, 15 Jan 2022 18:37:28: 37000000 INFO @ Sat, 15 Jan 2022 18:37:31: 39000000 INFO @ Sat, 15 Jan 2022 18:37:34: 30000000 INFO @ Sat, 15 Jan 2022 18:37:36: 38000000 INFO @ Sat, 15 Jan 2022 18:37:37: 40000000 INFO @ Sat, 15 Jan 2022 18:37:42: 31000000 INFO @ Sat, 15 Jan 2022 18:37:43: 39000000 INFO @ Sat, 15 Jan 2022 18:37:44: 41000000 INFO @ Sat, 15 Jan 2022 18:37:50: 32000000 INFO @ Sat, 15 Jan 2022 18:37:51: 42000000 INFO @ Sat, 15 Jan 2022 18:37:51: 40000000 INFO @ Sat, 15 Jan 2022 18:37:57: 43000000 INFO @ Sat, 15 Jan 2022 18:37:58: 33000000 INFO @ Sat, 15 Jan 2022 18:37:59: 41000000 INFO @ Sat, 15 Jan 2022 18:38:04: 44000000 INFO @ Sat, 15 Jan 2022 18:38:05: 34000000 INFO @ Sat, 15 Jan 2022 18:38:06: 42000000 INFO @ Sat, 15 Jan 2022 18:38:10: 45000000 INFO @ Sat, 15 Jan 2022 18:38:13: 35000000 INFO @ Sat, 15 Jan 2022 18:38:14: 43000000 INFO @ Sat, 15 Jan 2022 18:38:17: 46000000 INFO @ Sat, 15 Jan 2022 18:38:20: 36000000 INFO @ Sat, 15 Jan 2022 18:38:20: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:38:20: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:38:20: #1 total tags in treatment: 17549261 INFO @ Sat, 15 Jan 2022 18:38:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:38:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:38:21: #1 tags after filtering in treatment: 11262505 INFO @ Sat, 15 Jan 2022 18:38:21: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 18:38:21: #1 finished! INFO @ Sat, 15 Jan 2022 18:38:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:38:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:38:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:38:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:38:21: Process for pairing-model is terminated! INFO @ Sat, 15 Jan 2022 18:38:21: 44000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:38:27: 37000000 INFO @ Sat, 15 Jan 2022 18:38:28: 45000000 INFO @ Sat, 15 Jan 2022 18:38:34: 38000000 INFO @ Sat, 15 Jan 2022 18:38:35: 46000000 INFO @ Sat, 15 Jan 2022 18:38:39: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:38:39: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:38:39: #1 total tags in treatment: 17549261 INFO @ Sat, 15 Jan 2022 18:38:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:38:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:38:40: #1 tags after filtering in treatment: 11262505 INFO @ Sat, 15 Jan 2022 18:38:40: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 18:38:40: #1 finished! INFO @ Sat, 15 Jan 2022 18:38:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:38:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:38:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:38:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:38:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:38:41: 39000000 INFO @ Sat, 15 Jan 2022 18:38:47: 40000000 INFO @ Sat, 15 Jan 2022 18:38:53: 41000000 INFO @ Sat, 15 Jan 2022 18:38:59: 42000000 INFO @ Sat, 15 Jan 2022 18:39:06: 43000000 INFO @ Sat, 15 Jan 2022 18:39:12: 44000000 INFO @ Sat, 15 Jan 2022 18:39:18: 45000000 INFO @ Sat, 15 Jan 2022 18:39:24: 46000000 INFO @ Sat, 15 Jan 2022 18:39:27: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:39:27: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:39:27: #1 total tags in treatment: 17549261 INFO @ Sat, 15 Jan 2022 18:39:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:39:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:39:28: #1 tags after filtering in treatment: 11262505 INFO @ Sat, 15 Jan 2022 18:39:28: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 18:39:28: #1 finished! INFO @ Sat, 15 Jan 2022 18:39:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:39:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:39:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:39:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:39:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786278/SRX9786278.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling