Job ID = 14519817 SRX = SRX9786277 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 21512327 spots for SRR13362141/SRR13362141.sra Written 21512327 spots for SRR13362141/SRR13362141.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:03 21512327 reads; of these: 21512327 (100.00%) were paired; of these: 9751893 (45.33%) aligned concordantly 0 times 10703975 (49.76%) aligned concordantly exactly 1 time 1056459 (4.91%) aligned concordantly >1 times ---- 9751893 pairs aligned concordantly 0 times; of these: 201258 (2.06%) aligned discordantly 1 time ---- 9550635 pairs aligned 0 times concordantly or discordantly; of these: 19101270 mates make up the pairs; of these: 10930956 (57.23%) aligned 0 times 7319135 (38.32%) aligned exactly 1 time 851179 (4.46%) aligned >1 times 74.59% overall alignment rate Time searching: 00:11:03 Overall time: 00:11:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 244066 / 11960104 = 0.0204 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:10:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:10:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:10:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:10:32: 1000000 INFO @ Sat, 15 Jan 2022 18:10:38: 2000000 INFO @ Sat, 15 Jan 2022 18:10:44: 3000000 INFO @ Sat, 15 Jan 2022 18:10:50: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:10:56: 5000000 INFO @ Sat, 15 Jan 2022 18:10:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:10:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:10:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:11:02: 6000000 INFO @ Sat, 15 Jan 2022 18:11:02: 1000000 INFO @ Sat, 15 Jan 2022 18:11:09: 7000000 INFO @ Sat, 15 Jan 2022 18:11:09: 2000000 INFO @ Sat, 15 Jan 2022 18:11:15: 8000000 INFO @ Sat, 15 Jan 2022 18:11:15: 3000000 INFO @ Sat, 15 Jan 2022 18:11:22: 9000000 INFO @ Sat, 15 Jan 2022 18:11:22: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:11:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:11:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:11:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:11:28: 10000000 INFO @ Sat, 15 Jan 2022 18:11:28: 5000000 INFO @ Sat, 15 Jan 2022 18:11:32: 1000000 INFO @ Sat, 15 Jan 2022 18:11:35: 11000000 INFO @ Sat, 15 Jan 2022 18:11:35: 6000000 INFO @ Sat, 15 Jan 2022 18:11:38: 2000000 INFO @ Sat, 15 Jan 2022 18:11:42: 12000000 INFO @ Sat, 15 Jan 2022 18:11:42: 7000000 INFO @ Sat, 15 Jan 2022 18:11:44: 3000000 INFO @ Sat, 15 Jan 2022 18:11:48: 13000000 INFO @ Sat, 15 Jan 2022 18:11:48: 8000000 INFO @ Sat, 15 Jan 2022 18:11:50: 4000000 INFO @ Sat, 15 Jan 2022 18:11:55: 14000000 INFO @ Sat, 15 Jan 2022 18:11:55: 9000000 INFO @ Sat, 15 Jan 2022 18:11:56: 5000000 INFO @ Sat, 15 Jan 2022 18:12:01: 15000000 INFO @ Sat, 15 Jan 2022 18:12:01: 10000000 INFO @ Sat, 15 Jan 2022 18:12:02: 6000000 INFO @ Sat, 15 Jan 2022 18:12:08: 16000000 INFO @ Sat, 15 Jan 2022 18:12:08: 11000000 INFO @ Sat, 15 Jan 2022 18:12:09: 7000000 INFO @ Sat, 15 Jan 2022 18:12:14: 17000000 INFO @ Sat, 15 Jan 2022 18:12:15: 8000000 INFO @ Sat, 15 Jan 2022 18:12:15: 12000000 INFO @ Sat, 15 Jan 2022 18:12:21: 9000000 INFO @ Sat, 15 Jan 2022 18:12:21: 18000000 INFO @ Sat, 15 Jan 2022 18:12:21: 13000000 INFO @ Sat, 15 Jan 2022 18:12:27: 10000000 INFO @ Sat, 15 Jan 2022 18:12:28: 14000000 INFO @ Sat, 15 Jan 2022 18:12:28: 19000000 INFO @ Sat, 15 Jan 2022 18:12:33: 11000000 INFO @ Sat, 15 Jan 2022 18:12:34: 15000000 INFO @ Sat, 15 Jan 2022 18:12:34: 20000000 INFO @ Sat, 15 Jan 2022 18:12:39: 12000000 INFO @ Sat, 15 Jan 2022 18:12:41: 16000000 INFO @ Sat, 15 Jan 2022 18:12:41: 21000000 INFO @ Sat, 15 Jan 2022 18:12:45: 13000000 INFO @ Sat, 15 Jan 2022 18:12:47: 17000000 INFO @ Sat, 15 Jan 2022 18:12:48: 22000000 INFO @ Sat, 15 Jan 2022 18:12:51: 14000000 INFO @ Sat, 15 Jan 2022 18:12:54: 18000000 INFO @ Sat, 15 Jan 2022 18:12:54: 23000000 INFO @ Sat, 15 Jan 2022 18:12:57: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:13:01: 24000000 INFO @ Sat, 15 Jan 2022 18:13:01: 19000000 INFO @ Sat, 15 Jan 2022 18:13:03: 16000000 INFO @ Sat, 15 Jan 2022 18:13:07: 20000000 INFO @ Sat, 15 Jan 2022 18:13:07: 25000000 INFO @ Sat, 15 Jan 2022 18:13:09: 17000000 INFO @ Sat, 15 Jan 2022 18:13:13: 21000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:13:14: 26000000 INFO @ Sat, 15 Jan 2022 18:13:15: 18000000 INFO @ Sat, 15 Jan 2022 18:13:20: 22000000 INFO @ Sat, 15 Jan 2022 18:13:21: 19000000 INFO @ Sat, 15 Jan 2022 18:13:21: 27000000 INFO @ Sat, 15 Jan 2022 18:13:26: 23000000 INFO @ Sat, 15 Jan 2022 18:13:27: 20000000 INFO @ Sat, 15 Jan 2022 18:13:28: 28000000 INFO @ Sat, 15 Jan 2022 18:13:33: 21000000 INFO @ Sat, 15 Jan 2022 18:13:33: 24000000 INFO @ Sat, 15 Jan 2022 18:13:35: 29000000 INFO @ Sat, 15 Jan 2022 18:13:39: 22000000 INFO @ Sat, 15 Jan 2022 18:13:40: 25000000 INFO @ Sat, 15 Jan 2022 18:13:41: 30000000 INFO @ Sat, 15 Jan 2022 18:13:45: 23000000 INFO @ Sat, 15 Jan 2022 18:13:47: 26000000 INFO @ Sat, 15 Jan 2022 18:13:48: 31000000 INFO @ Sat, 15 Jan 2022 18:13:51: 24000000 INFO @ Sat, 15 Jan 2022 18:13:52: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:13:52: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:13:52: #1 total tags in treatment: 11517116 INFO @ Sat, 15 Jan 2022 18:13:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:13:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:13:52: #1 tags after filtering in treatment: 8394389 INFO @ Sat, 15 Jan 2022 18:13:52: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 18:13:52: #1 finished! INFO @ Sat, 15 Jan 2022 18:13:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:13:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:13:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:13:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:13:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:13:54: 27000000 INFO @ Sat, 15 Jan 2022 18:13:57: 25000000 INFO @ Sat, 15 Jan 2022 18:14:01: 28000000 INFO @ Sat, 15 Jan 2022 18:14:03: 26000000 INFO @ Sat, 15 Jan 2022 18:14:08: 29000000 INFO @ Sat, 15 Jan 2022 18:14:08: 27000000 INFO @ Sat, 15 Jan 2022 18:14:14: 28000000 INFO @ Sat, 15 Jan 2022 18:14:14: 30000000 INFO @ Sat, 15 Jan 2022 18:14:20: 29000000 INFO @ Sat, 15 Jan 2022 18:14:21: 31000000 INFO @ Sat, 15 Jan 2022 18:14:25: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:14:25: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:14:25: #1 total tags in treatment: 11517116 INFO @ Sat, 15 Jan 2022 18:14:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:14:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:14:25: #1 tags after filtering in treatment: 8394389 INFO @ Sat, 15 Jan 2022 18:14:25: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 18:14:25: #1 finished! INFO @ Sat, 15 Jan 2022 18:14:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:14:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:14:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:14:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:14:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:14:26: 30000000 INFO @ Sat, 15 Jan 2022 18:14:32: 31000000 INFO @ Sat, 15 Jan 2022 18:14:35: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:14:35: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:14:35: #1 total tags in treatment: 11517116 INFO @ Sat, 15 Jan 2022 18:14:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:14:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:14:35: #1 tags after filtering in treatment: 8394389 INFO @ Sat, 15 Jan 2022 18:14:35: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 18:14:35: #1 finished! INFO @ Sat, 15 Jan 2022 18:14:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:14:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:14:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:14:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:14:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786277/SRX9786277.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling