Job ID = 14519815 SRX = SRX9786275 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10679755 spots for SRR13362139/SRR13362139.sra Written 10679755 spots for SRR13362139/SRR13362139.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:40 10679755 reads; of these: 10679755 (100.00%) were paired; of these: 6157056 (57.65%) aligned concordantly 0 times 3624386 (33.94%) aligned concordantly exactly 1 time 898313 (8.41%) aligned concordantly >1 times ---- 6157056 pairs aligned concordantly 0 times; of these: 22781 (0.37%) aligned discordantly 1 time ---- 6134275 pairs aligned 0 times concordantly or discordantly; of these: 12268550 mates make up the pairs; of these: 8625742 (70.31%) aligned 0 times 2897148 (23.61%) aligned exactly 1 time 745660 (6.08%) aligned >1 times 59.62% overall alignment rate Time searching: 00:09:40 Overall time: 00:09:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 381399 / 4544491 = 0.0839 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:04:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:04:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:04:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:04:21: 1000000 INFO @ Sat, 15 Jan 2022 18:04:30: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:04:39: 3000000 INFO @ Sat, 15 Jan 2022 18:04:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:04:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:04:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:04:49: 4000000 INFO @ Sat, 15 Jan 2022 18:04:50: 1000000 INFO @ Sat, 15 Jan 2022 18:04:58: 2000000 INFO @ Sat, 15 Jan 2022 18:04:59: 5000000 INFO @ Sat, 15 Jan 2022 18:05:07: 3000000 INFO @ Sat, 15 Jan 2022 18:05:08: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:05:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:05:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:05:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:05:16: 7000000 INFO @ Sat, 15 Jan 2022 18:05:16: 4000000 INFO @ Sat, 15 Jan 2022 18:05:20: 1000000 INFO @ Sat, 15 Jan 2022 18:05:25: 8000000 INFO @ Sat, 15 Jan 2022 18:05:25: 5000000 INFO @ Sat, 15 Jan 2022 18:05:29: 2000000 INFO @ Sat, 15 Jan 2022 18:05:34: 9000000 INFO @ Sat, 15 Jan 2022 18:05:34: 6000000 INFO @ Sat, 15 Jan 2022 18:05:38: 3000000 INFO @ Sat, 15 Jan 2022 18:05:42: 10000000 INFO @ Sat, 15 Jan 2022 18:05:43: 7000000 INFO @ Sat, 15 Jan 2022 18:05:46: 4000000 INFO @ Sat, 15 Jan 2022 18:05:50: 11000000 INFO @ Sat, 15 Jan 2022 18:05:52: 8000000 INFO @ Sat, 15 Jan 2022 18:05:55: 5000000 INFO @ Sat, 15 Jan 2022 18:05:58: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:05:58: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:05:58: #1 total tags in treatment: 4141782 INFO @ Sat, 15 Jan 2022 18:05:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:05:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:05:58: #1 tags after filtering in treatment: 2544301 INFO @ Sat, 15 Jan 2022 18:05:58: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 18:05:58: #1 finished! INFO @ Sat, 15 Jan 2022 18:05:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:05:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:05:58: #2 number of paired peaks: 79 WARNING @ Sat, 15 Jan 2022 18:05:58: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:05:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:06:01: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:06:05: 6000000 INFO @ Sat, 15 Jan 2022 18:06:10: 10000000 INFO @ Sat, 15 Jan 2022 18:06:13: 7000000 INFO @ Sat, 15 Jan 2022 18:06:19: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:06:22: 8000000 INFO @ Sat, 15 Jan 2022 18:06:27: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:06:27: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:06:27: #1 total tags in treatment: 4141782 INFO @ Sat, 15 Jan 2022 18:06:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:06:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:06:27: #1 tags after filtering in treatment: 2544301 INFO @ Sat, 15 Jan 2022 18:06:27: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 18:06:27: #1 finished! INFO @ Sat, 15 Jan 2022 18:06:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:06:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:06:28: #2 number of paired peaks: 79 WARNING @ Sat, 15 Jan 2022 18:06:28: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:06:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:06:31: 9000000 INFO @ Sat, 15 Jan 2022 18:06:39: 10000000 INFO @ Sat, 15 Jan 2022 18:06:48: 11000000 INFO @ Sat, 15 Jan 2022 18:06:56: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:06:56: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:06:56: #1 total tags in treatment: 4141782 INFO @ Sat, 15 Jan 2022 18:06:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:06:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:06:56: #1 tags after filtering in treatment: 2544301 INFO @ Sat, 15 Jan 2022 18:06:56: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 18:06:56: #1 finished! INFO @ Sat, 15 Jan 2022 18:06:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:06:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:06:57: #2 number of paired peaks: 79 WARNING @ Sat, 15 Jan 2022 18:06:57: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:06:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786275/SRX9786275.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling